Dusp27 (NM_001191929) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	RGD1560598
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217497 representing NM_001191929 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTACCAGTGGAGACACAGAGGAGGAGCAGGTAGTTCCAAACGAGGAGGATGAAGCCGACGTGAGAG CTGTACAGGCCCACTACCTCCGGAGTCCCTCCCCAAGCCAGTACTCAGTGGTCTCAGACGCAGAAACTGA AAGTATTTTCATGGAGCCCATCCATCTCTCCTCGGCCGTAGCTGCCAAGCAGATCATCAACGAAGAACTC AAGCCTCGGGGTCTCAGAACAGACACGGAGTGCCCAGGCATGCTGGAGTCTGCTGAACAGCTGCTGGTGG AAGACCTGTACAACCGTGTCCGGGAGAAGATGGATGACAAAAGCCTGTTCAACACCCCCTGTGTGCTGGA CCTGCAGCGGGCACTGATCCAGGACCGGCAAGAGGCCCCTCGGAATGAGGTGGACGAGGTCTGGCCCAAC GTCTTCATAGCTGAGAAGAGTGTGGCTGTGAACAAGGGGCGACTGAAAAGGCTGGGAATCACCCACATCC TGAACGCTGCCCATGGCACTGGCGTTTACACTGGTCCCGAATTCTACACTGGCCTGGAGATCCAGTACCT GGGGGTGGAAGTGGATGATTTCCCAGAGGTGGACATCTCCCAGCATTTCCGGAAGGCAGCTGAGTTTCTT GATGAAGCCCTGCTGACCTATAGAGGGAAAGTCCTGGTCAGCAGTGAGATGGGTATCAGCAGGTCAGCAG TGCTGGTGGTCGCCTACCTGATGATCTTCCACAACATGGCCATCCTGGAAGCCTTGATGACTGTCCGCCG AAAGCGGGCTATCTACCCCAACGACGGCTTCCTCAAACAGCTGCGGGAGCTCAACGAGAAACTGATGGAG GAGAGGGAAGAAGAGGATGATGAAGAGGAGCCTGAGGAGGATGCCGGGAGCACACTGGGTGCTAGAGTAA ATTCCTTGATGGTGGAAGAAGAAGATGATGCCACCAGCCACCTGAGTGGCTCCTCCTTGGGGAAGGCCAG CCAGGTCTCCCAGCCAGTCACCCTCATTGATGAAGAGGAGGAAGAGAGGCTGTATGAGGAGTGGAGGAAG GGGCAGGGCTTCCCCAAGGCTGAGGCTTCTCAGGGTAGAAAGGGTAGCTGTTCTGCGTCCTCCGAGCAAG ACGGGGACGACTGTGAGGATGGGGCTGTGGAGAGGATAATCCAGGAGTGGCAGAGCCGAAATGAGAGGTA CCAAGCCAAAGGGCACAAGCAGTGGAATCGGGAGGAAGACGAGGAGGAAGAGAACTCCTACACCAGCAGA AGGCGCAGACACACCCTAAGCGAGAGCAGCGCCTCTGAGAGCGTGAGCAGCCATGACATCCGGGTCCTGA AGCAGCAACTGAAGAGGACCAGCCAAAGCCGCCACGACAGACACCGCTCTGACTCCGAGTCCACGGAGAG CACCTGGGACATGTGGAACGAGAGGCTGGTGGAGATCGAGAAGGAGGCTGCCCGGAAGTATCGCTCCAAG AGCAAGAGGGAGGAGCTGGATGCCGATTCAGAGGCAGGGAGCAGGGTCCGAGAGGACGATGAGGAGAGTG TATTGTCCGAGGCCAGCTCCTTCTACAACTTCTGCAGTAGGAACAAGGACAAGCTCACTGCCCTGGAAAG GTGGAAGGTTAAGAGAATCCAATTTGGATTTCACAAGAAAGACTCAGAGGTGGTAGACGCGGGCAGTGAG GATGGTACGGAAGAGGCAACAGGAGAGAAGAACCTTTCTGATGTCAACCTGACAGCCTACCAGGCCTGGA AGCTGAAACACCAGAAGAAGGTGGGCAATGAGAACAAGGAGGAGGTGGTTGAGATGAGCAAAGGAGAGGA CACAGCCTTAGCTAAGAAGAGACAACGGAGACTAGAGTTACTGGAGAGAAGCAGGCAGACGCTGGAGGAG AGCCGGTCCATGGGAAGCTGGGAGGGAGACAGCTCAACTGCCAGCAGGAGCATCCCCCTGTCTGCATTCT GGTCTGCAGCCCCCTCTGTCAGTGTGGATGGGGACACAGCGTCAGTACTCAGCGCCCAGAGCCAGCATTC TCACCTGTCTCAGCCTGCAAGTAACATGCCCACCACACCCCTGCCTAACCTGCCAGTGGGTCCGGGAGAC ACCATTTCCATTGCCAGTATCCAGAACTGGATCGCCAGTGTCGTCAATGAAACCCTTGCCCAAAAGCAAA ACGAAATGCTCACGTTGTCCCGCGCCCCCTCTGTTGCAAGCATGAGGGCAGCTCCAGCAGCCAGCTGCCT TGGGGATGACCAACTCTCGGTGCTCAGCACCTCCCTGAGTGGCTGCCTACCGCCTCAGAGCCAGGGGAGA CCCAGCTCCGACGTGCAATCTGTGCTGTCCTCCACCAGCTCCCTGACCTCCAGGGCTGAAGGCAGTGAGA ACAAAGTGAGAGGGACCAGCAAGCCCATCTACAGCCTCTTTGCTGACAACGTGAACCTGAAGGAGCTCGG CCGGAAGGAGAAAGAAATGCAGATGGAGCTGCAGGAGAAGATGTCCGAATATAAAATGGAGAAGCTGGCC TCTGATAACAAGCGTAGCTCTCTCTTCAAGAAGAAGAAGGCCAAGGACGACGAGGACATCAGCGTGGGTG ACAGAGACGAGGACACAGACAGTGCCATTGGAAGCTTCCGGTATTCCTCCCGAAGTAATTCCCAGAAGCC TGAGACAGATGCCTCCTCCTCTCTGGCCATCTCCGATCACTTTAGAAATGGCCGCAGCGTGGGCAACGAG GTAGGTAGCAATATTAATACATGGCTCAGTGGCCTCAGGATGGAAGAAAAATCTCCTCCTCAAAGTGATT GGTCTGGAAGCTCCAGGGGGAGGTACTCCAGGTCTTCCCTGTTCCGGGAAACAGAGTCTAAATCCTGCAG TTACAAGTTCTCCAAATCTAGGTCACAGGAACAGGACTCCTCCTTCCACGAAACCGATGGCAACACTGTG CGAAACACCTCCCGGTTCTCCTCTTCCACCACCAAGGAGGGCAGAGAGATGCACAAGTTCTCCAGGTCCA CATTCAGCGAGACCTCGAGCTCCCGAGAGGAGAGCCCAGAACCCTATTTCTTCCGCCGAACCCCTGAACC GTCCGATGGGGAAGAGTCCCCCGAGCTAAGGCGTCCAAACTGGACCAGGCCCAGGGATTGGGAAGACGTA GAAGAGTCATCTAAATCAGACTTCGCTGAGTTTGGGGCCAAGAGGAAATTCACCCAGAGCTTCATGAGGT CTGAAGAGGAGGGAGAGAAGGAGAGGACAGAAAACCGAGAGGAAGGGAGGTTTGCATCTGGGCGTCAGTC CCAGTATCGGAGAAGCACAAACCGGCAAGAGGAAGAAGAAATGGATGATGAAGCCATCATTGCTGCTTGG AGAAAGCGGCAAGAAGAAACCAGAACCAAACTGCAAAGAAGGAGAGAGGACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001191929
Insert Size	3414 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001191929.1, NP_001178858.1
RefSeq Size	3899 bp
RefSeq ORF	3414 bp
Locus ID	498267

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