Lats1 (NM_001134543) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	RGD1564085
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217489 representing NM_001134543 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGAGGGGTGAAAAGCCAGAAGGGTACAGACAGATGAGGCCTAAGACCTTTCCTGCCAGCAACTACT CTGGCAGCAGCCGACAAATGCTGCAGGAGATCCGAGAATCCCTTAGGAATTTATCAAAGCCATCTGATGC CGCAAAGGCCGAGCATAACCTGAACAAGATGTCGACCGAAGATCCCAGGCAGGTGAGAAATCCGCCCAAA TTTGGCACACATCATAAAGCCTTGCAAGAAATTCGAAACTCTCTACTACCTTTCGCAAACGAGACGAGTT CTTCCCGGATCCCTTCAGAAGTTAATCCACAGATGTTTCAGGATTTGCAGGCTGCTGGCTTTGATGAGGA TATGGTTATTCAAGCTCTTCAGAAAACCAATAACAGAAGCATAGAAGCAGCTGTTGAATTCATTAGTAAA ATGAGTTATCAGGATCCTCGTCGAGAGCAGATGGCTGCAGCAGCTGCCAGACCTATCAATGCCACCATGA AACCAGGAAATGTGCAACATTCAATTAGCCGAAAACAAAGCTGGAAAGGTTCTAAAGAGTCTTTAGTTCC TCAGAGACACGGTCCATCTCTAGGAGAAAATGTGGTTTATCGATCTGAAAGTCCCAACTCACAGGCGGAT GTAGGAAGACCTCTGTCTGGATCCGGCATTACGGCATTTGCTCAAGCTCACCCAAGCAATGGACAGAGAG TGAACCCACCACCACCACCTCAAGTTAGGAGTGTTACTCCTCCACCACCTCCACGAGGGCAGACCCCACC TCCACGAGGCACAACTCCCCCTCCCCCCTCGTGGGAGCCTAACTCTCAGACAAAGCGCTACTCTGGGAAC ATGGAATATGTAATCTCCCGAATCTCTCCTGTTCCACCTGGGGCGTGGCAGGAGGGCTACCCTCCACCAC CTCTTAACACTTCCCCCATGAATCCCCCCAACCAGGGACAGAGAGGCATCAGTTCTGTTCCAGTTGGCAG GCAACCAATCATCATGCAAAGTACTAGCAAATTTAACTTTACACCAGGGAGACCTGGAGTTCAGAATGGT GGTGGTCAGACTGATTTTATCATGCACCAAAATGTTGTCCCCACTGGTGCTGTGAATCGGCAACCACCAC CACCATATCCTCTGACCCCAGCTAATGGACAAAGCCCTTCTGCTTTGCAAGCAGGCGGTCCTGCTGCTCC ACCATCATTCTCCAATGGAAATGTTCCTCCAACGATGATGGTGCCGAACAGGAACAGTCATAACATGGAG CTGTATAATATCAATGTCCCTGGACTGCAAACAACTTGGCCCCAGTCGTCTTCTGCCCCTGTGCAATCAT CTCCAAGCAGTGGGCATGAAATTCCTACGTGGCAACCTAACATACCAGTGAGGTCAAATTCTTTTAATAA CCCACTAGGAAGTAGAGCAAGTCACTCTGCTAATTCTCAGCCTTCCGCCACGACAGTTACTGCCATCACA CCAGCTCCTATTCAGCAGCCCGTGAAAAGCATGCGCGTTCTGAAACCAGAGCTGCAGACTGCCTTAGCCC CAACCCATCCTTCTTGGATGCCACAGCCAGTTCAGACCGTTCAGCCTAGCCCTTTTTCTGAGGGTGCAGC TTCAAACGTGCCAGTCATCCCACCTGTGGCGGAAGCTCCAAGCTATCAGGGCCCACCACCACCTTATCCA AAACATCTGCTACACCAAACCCCATCTGTTCCTCCATATGAGTCAGCGAGTAAACCCTGCAAAGACGATC AGCCTAGCTCAGCCAAGGAAGAGGATAGTGAAAAGAGTTTGGACAGTGTTGACTCTGGGGATAAAGAGAA GAAACAGATTACAACTTCACCTATCACTGTTCGGAAAAACAAGAAAGACGAAGAGCGAAGAGAGTCTCGC ATTCAGAGTTACTCCCCGCAGGCCTTTAAATTCTTCATGGAGCAACACGTAGAGAACGTCCTGAAGTCTC ATCAGCAGCGTCTGCATCGCAAGAAGCAACTAGAGAATGAAATGATGCGGGTTGGATTATCTCAAGATGC CCAGGATCAAATGAGAAAGATGCTTTGCCAGAAAGAGTCTAACTATATTCGTCTTAAAAGGGCTAAAATG GACAAGTCTATGTTTGTAAAGATAAAGACATTAGGAATAGGAGCATTTGGTGAAGTCTGTCTAGCAAGAA AAGTCGATACGAAAGCTTTGTATGCAACAAAAACTCTACGAAAGAAAGACGTTCTGCTCCGAAATCAGGT GGCTCATGTGAAAGCTGAGCGAGATATCCTAGCAGAAGCTGACAACGAGTGGGTGGTCCGCTTGTACTAC TCATTCCAGGACAAGGACAACTTGTACTTTGTGATGGACTACATTCCTGGGGGTGATATGATGAGCCTGT TAATTAGAATGGGCATCTTTCCCGAAAACCTGGCACGATTCTACATAGCAGAACTTACCTGTGCAGTTGA AAGTGTTCATAAAATGGGTTTTATTCATAGAGACATTAAACCTGATAACATTTTGATTGACCGAGATGGA CATATTAAATTGACTGACTTTGGCTTGTGCACTGGCTTCAGATGGACACATGACTCCAAGTACTACCAGA GTGGGGATCACCCACGGCAAGATAGCATGGATTTCAGTAGCGAATGGGGAGATCCTTCCAACTGTCGGTG TGGGGACAGACTGAAGCCACTAGAGCGGAGAGCTGCTCGCCAGCACCAGCGATGTCTAGCTCACTCTCTG GTTGGGACTCCCAACTATATAGCACCTGAAGTGCTACTACGGACAGGATATACACAGCTGTGTGATTGGT GGAGTGTTGGTGTTATTCTTTTTGAAATGTTGGTGGGACAACCTCCTTTCTTGGCACAAACACCATTAGA AACCCAAATGAAGGTTATCAACTGGCAAACTTCTCTTCACATCCCACCTCAAGCCAAGCTCAGTCCTGAA GCCTCTGACCTCATTATCAAACTTTGCCGAGGACCAGAAGACCGTCTAGGCAAGAACGGTGCTGATGAAA TAAAGGCTCATCCATTTTTTAAGACCATTGATTTCTCTAGTGATCTGAGACAGCAGTCTGCTTCATACAT CCCCAAAATCACGCACCCAACAGACACGTCCAATTTTGACCCTGTTGACCCTGATAAGTTGTGGAGCGAT GGTAACGAGGAAGAAAATATCAACGACACTCTGAACGGATGGTATAAAAACGGAAAGCACCCTGAGCATG CTTTCTATGAGTTTACCTTTCGGAGGTTCTTTGATGACAATGGCTACCCATATAATTATCCAAAGCCCAT CGAATATGAATACGTTAGTTCCCAGGGCTCAGAGCAGCAGTCCGATGAAGACGATCAACACACAGGCTCA GATGTAAACAACCGTGATCTAGTGTATGTTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001134543
Insert Size	3393 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001134543.2, NP_001128015.2
RefSeq Size	7087 bp
RefSeq ORF	3393 bp
Locus ID	308265

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