Grin3a (NM_001198583) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	chi-1; GluN3A; NR3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217485 representing NM_001198583 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGAGACTGAGTTTGTGGTGGCTGCTGAGCAGGGTCTGTCTGCTGCTGCCGCCGCCCTGCGCACTGG TGCTGGCCGGGGTGCCCAGCTCCTCCTCGCACCCGCAACCCTGCCAGATCCTCAAGCGCATCGGACACGC GGTGAGGGTGGGCGCGGTGCACTTGCAACCCTGGACCACGGCCCCACGCGCAGCCAGTCGCGCTCAGGAA GGCGGCAGGGCGGGTGCCCAGAGGGATGATCCAGAGTCCGGGACGTGGCGGCCACCGGCGCCCTCGCAAG GCGCACGCTGGTTGGGGAGCGCCCTGCATGGCCGGGGTCCACCCGGCTCCCGAAAGCTCGGGGAGGGCGC GGGGGCCGAGACCCTGTGGCCGCGGGATGCCCTACTGTTCGCTGTGGAAAACTTGAACCGTGTGGAAGGG CTCCTACCCTACAACCTGTCTTTGGAAGTAGTGATGGCCATTGAGGCGGGCCTGGGCGATCTGCCGCTTA TGCCCTTCTCTTCCCCAAGCTCACCGTGGAGCAGTGACCCTTTCTCCTTTCTGCAGAGCGTGTGCCACAC CGTAGTGGTACAAGGGGTGTCGGCGCTGCTGGCCTTCCCCCAGAGCCAGGGCGAAATGATGGAGCTGGAC TTGGTCAGCTCTGTCCTGCACATCCCAGTGCTCAGCATAGTGCGCCACGAGTTTCCGCGGGAGAGTCAGA ATCCCCTGCACCTACAGCTGAGTTTAGAAAACTCACTAAGTTCTGATGCTGATGTCACTGTCTCAATCCT GACCATGAACAACTGGTACAATTTTAGCTTGTTGCTCTGCCAGGAAGACTGGAATATCACGGACTTCCTC CTCCTTACGGAGAATAACTCCAAGTTCCACCTCGAGTCTGTTATCAACATCACTGCTAATCTGTCCTCCA CAAAGGACCTTCTAAGTTTCCTGCAGGTCCAGATGGACAACATTAGGAACAGCACACCCACAATGGTGAT GTTTGGCTGTGACATGGACAGTATCCGGCAGATATTTGAAATGTCCACACAGTTTGGGCTATCACCTCCT GAACTTCACTGGGTTTTAGGAGACTCCCAGAATGTGGAGGAACTGAGGACAGAAGGCCTGCCCTTAGGGC TCATTGCTCATGGAAAAACCACACAATCTGTCTTTGAGTACTACGTTCAGGATGCCATGGAGTTGGTTGC AAGAGCTGTAGCCACAGCCACCATGATCCAGCCAGAGCTTGCTCTCCTTCCCAGCACAATGAACTGCATG GATGTGAAAACCACAAATCTCACTTCTGGACAGTATTTATCAAGGTTTTTAGCCAACACCACTTTCAGAG GTCTCAGTGGTTCCATCAAAGTAAAGGGCTCCACCATCATCAGCTCAGAGAACAACTTTTTCATCTGGAA CTTGCAGCACGACCCTATGGGAAAGCCAATGTGGACTCGCCTGGGCAGCTGGCAAGGAGGAAGGATAGTC ATGGACTCTGGAATATGGCCAGAGCAGGCCCAGAGGCACAAAACCCACTTCCAGCACCCAAACAAGTTAC ACTTGAGAGTGGTGACATTGATTGAACACCCATTTGTTTTCACGAGAGAAGTAGACGATGAAGGCTTATG CCCTGCTGGACAGCTCTGTCTAGACCCTATGACTAACGACTCTTCCATGCTGGATCGTCTGTTTAGCAGC CTGCATAGCAGTAATGACACAGTGCCAATCAAGTTCAAGAAGTGCTGTTATGGGTACTGCATCGATCTCC TGGAGCAGCTAGCAGAAGACATGAACTTTGACTTTGACCTCTACATTGTAGGGGATGGAAAGTACGGAGC TTGGAAAAATGGTCACTGGACTGGGCTGGTTGGTGATCTCCTGAGTGGGACAGCCAACATGGCGGTCACT TCTTTCAGCATCAATACCGCACGAAGCCAGGTGATAGATTTCACCAGCCCTTTCTTCTCAACCAGTTTGG GGATCTTAGTGAGGACTCGAGACACAGCAGCTCCAATTGGAGCCTTCATGTGGCCACTCCACTGGACCAT GTGGCTGGGAATTTTCGTGGCTCTCCATATCACTGCCATTTTTCTCACTCTGTATGAATGGAAGAGCCCC TTTGGTATGACCCCTAAGGGGAGGAACAGAAACAAAGTCTTCTCCTTCTCCTCAGCTTTGAATGTCTGCT ATGCCCTTCTGTTTGGCAGAACAGCAGCCATCAAACCCCCAAAATGCTGGACTGGAAGGTTTCTGATGAA TCTTTGGGCCATTTTCTGTATGTTTTGCCTTTCTACATACACAGCGAACTTGGCTGCTGTCATGGTAGGT GAGAAGATCTATGAAGAGCTTTCTGGAATTCATGACCCTAAGCTTCATCATCCTTCTCAAGGCTTCCGCT TTGGAACTGTCCGGGAAAGCAGTGCTGAAGACTATGTGCGCCAGAGCTTCCCAGAGATGCATGAGTACAT GAGAAGGTACAACGTGCCAGCCACCCCTGATGGAGTGCAGTATCTGAAGAATGATCCAGAGAAACTAGAC GCCTTCATCATGGACAAAGCCCTTCTGGATTATGAAGTGTCAATAGATGCTGACTGCAAGCTTCTGACCG TAGGAAAGCCATTTGCCATCGAAGGATATGGCATTGGTCTCCCTCCAAACTCTCCATTGACCTCTAATAT ATCTGAGCTCATCAGTCAGTACAAGTCTCACGGGTTTATGGATGTGCTCCATGACAAGTGGTACAAGGTG GTTCCCTGCGGAAAGAGAAGCTTTGCCGTCACTGAGACTTTGCAAATGGGCATCAAGCACTTCTCTGGAC TCTTCGTGCTGTTGTGCATAGGATTTGGTCTCTCCATCCTGACCACCATTGGTGAACACATAGTGCACAG ACTGCTGTTACCACGCATCAAAAACAAATCCAAGCTGCAGTACTGGCTGCACACGAGTCAGAGGTTTCAC AGAGCATTAAACACGTCATTCGTAGAAGAAAAGCAGCCACGTTCCAAGACAAAACGTGTGGAGAAGAGGT CCAACCTGGGACCCCAGCAGCTCATGGTATGGAATACTTCCAATCTGAGTCATGACAACCAACGAAAATA CATCTTTAATGACGAGGAAGGACAAAACCAGCTGGGTACCCAGGCCCACCAGGACATCCCTCTCCCTCAG AGGAGAAGAGAGCTCCCTGCCTCACTGACCACCAATGGGAAAGCAGACTCCCTCAATGTAACTCGGAGCT CCGTGATTCAGGAACTCTCTGAGTTGGAGAAGCAGATCCAAGTGATCCGCCAGGAGCTGCAGTTGGCTGT AAGCAGGAAGACAGAGCTGGAGGAGTATCAAAAGACAAATCGGACTTGTGAATCCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001198583
Insert Size	3348 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001198583.2, NP_001185512.1
RefSeq Size	4326 bp
RefSeq ORF	3348 bp
Locus ID	191573
UniProt ID	Q9R1M7
Gene Summary	glutamate receptor subunit involved in neuronal signaling [RGD, Feb 2006] Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The resulting isoform (2) lacks an internal segment, compared to isoform 1.

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