Hdac5 (NM_053450) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217483 representing NM_053450 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACTCTCCCAACGAGTCGGATGGCATGTCAGGCCGGGAACCATCCTTGGAAATCTTGCCACGGACTC CTCTGCACAGCATCCCTGTGGCAGTGGAGGTGAAGCCGGTGCTGCCAGCAGCCATGCCCAGCTCCATGGG AGGTGGAGGTGGAGGTAGCCCCAGCCCTGTGGAGCTTCGGGGGGCTCTGGCAGGCCCCATGGACCCCGCG CTTCGGGAGCAGCAACTGCAGCAAGAGCTCCTGGTGCTCAAGCAGCAGCAGCAGCTGCAAAAGCAGCTCC TGTTTGCCGAGTTTCAGAAGCAGCACGACCACCTGACGCGGCAGCATGAAGTCCAGCTGCAGAAGCACCT CAAGCAGCAGCAGGAGATGCTGGCGGCTAAGAGGCAGCAGGAGCTGGAGCAGCAGCGGCAGCGCGAGCAG CAGCGGCAAGAGGAGCTAGAGAAACAGCGGCTGGAGCAGCAGCTGCTTATTCTACGAAACAAGGAGAAGA GCAAAGAGAGTGCCATCGCCAGCACCGAGGTAAAGCTGAGGCTCCAGGAATTCCTGTTGTCCAAGTCAAA GGAGCCCACACCAGGCGGCCTCAACCATTCCCTCCCACAGCACCCCAAATGCTGGGGAGCCCATCATGCT TCTTTGGACCAGAGTTCCCCTCCCCAGAGCGGCCCTCCTGGGACGCCTCCCTCCTACAAATTGCCTTTGC TTGGGCCCTATGACAGCCGTGATGACTTTCCCCTCCGTAAAACGGCCTCGGAACCCAACTTAAAAGTACG TTCAAGGCTAAAACAGAAGGTAGCTGAGAGAAGAAGCAGTCCCCTCCTGCGTCGAAAGGATGGCACTGTT ATTAGTACTTTTAAGAAGAGAGCAGTCGAGATCACAGGCACAGGGCCTGGGGTGTCGTCCGTGTGTAACA GTGCGCCTGGCTCTGGCCCCAGCTCTCCCAACAGTTCCCACAGCACCATCGCTGAGAACGGCTTTACTGG CTCAGTCCCCAACATCCCCACTGAGATGCTCCCCCAGCACCGGGCCCTCCCTCTGGACAGTTCCCCAAAC CAGTTCAGCCTCTACACGTCTCCTTCTCTGCCTAACATCTCCCTAGGGCTGCAGGCCACTGTCACTGTTA CCAACTCGCACCTCACCGCCTCCCCGAAGCTGTCGACACAGCAGGAGGCTGAGAGGCAGGCCCTTCAGTC CCTGCGGCAGGGCAGCACACTGACCGGCAAGTTCATGAGCACATCCTCCATCCCTGGCTGCCTGCTGGGA GTGGCCCTGGAGGGCGACACGAGCCCCCATGGGCACGCTTCCCTGCTGCAGCACGTTTTGCTGCTGGAGC AGGCCCGGCAACAGAGCACGCTCATAGCGGTGCCACTCCATGGGCAGTCCCCACTGGTGACGGGTGAACG TGTGGCCACCAGCATGAGGACAGTGGGCAAGCTCCCAAGGCACCGACCTCTGAGCCGTACTCAGTCCTCG CCCCTGCCGCAGAGTCCCCAGGCCCTGCAGCAGCTGGTTATGCAGCAGCAGCACCAGCAGTTCCTAGAGA AACAGAAGCAGCAGCAGATGCAGCTGGGCAAGATCCTTACCAAAACTGGGGAGCTGTCAAGGCAGCCCAC CACCCACCCTGAAGAGACAGAGGAGGAGCTGACGGAACAGCAGGAGGCCTTGCTGGGGGAGGGGGCCCTG ACCATGCCCCGGGAAGGTTCTACAGAGAGCGAGAGCACCCAGGAAGATCTAGAAGAGGAAGAGGATGAGG AGGAGGACGAGGAGGACTGCATTCAGGTCAAGGATGAGGATGGCGAGAGTGGTCCTGATGAGGGCGCAGA CTTGGAGGAGTCCAGTGCTGGTTACAAAAAGTTGTTCGCAGATGCCCAGCAGTTACAGCCCCTCCAGGTA TACCAGGCACCCCTCAGCCTGGCCACTGTGCCTCATCAGGCCCTGGGCCGCACCCAGTCCTCACCTGCTG CTCCTGGGAGCATGAAGAGCCCCACAGACCACCCCACTGTGGTGAAGCACCTCTTCACCACAGGTGTGGT CTATGACACGTTCATGCTGAAGCATCAGTGTATGTGCGGAAACACACATGTGCACCCAGAGCACGCCGGC CGCATCCAGAGCATCTGGTCCCGGCTGCAGGAAACTGGTCTGCTCAGCAAGTGTGAGCGGATCCGGGGTC GTAAAGCCACACTGGATGAAATCCAGACCGTGCACTCTGAGTACCACACCCTGCTCTATGGGACCAGTCC CCTTAACCGGCAGAAGCTGGACAGTAAGAAGCTGCTTGGCCCCATCAGCCAGAAGATGTACGCCATGCTG CCTTGTGGGGGCATTGGGGTGGACAGTGACACTGTGTGGAATGAGATGCACTCCTCTAGTGCCGTGCGAA TGGCAGTGGGCTGCCTGGTGGAGCTGGCCTTCAAGGTGGCTGCAGGAGAGCTCAAGAATGGATTTGCCAT CATCCGACCCCCAGGACATCATGCTGAGGAGTCCACAGCCATGGGATTCTGCTTCTTCAACTCCGTAGCC ATCACAGCTAAACTCCTGCAGCAGAAGCTGAGCGTGGGCAAGGTCCTCATCGTGGACTGGGACATTCACC ACGGCAATGGCACCCAGCAAGCATTTTACGATGACCCCTCCGTGCTCTACATCTCTTTGCATCGCTACGA CAATGGGAACTTCTTTCCAGGCTCCGGGGCTCCTGAAGAGGTTGGTGGAGGGCCAGGTGTGGGGTACAAC GTAAATGTGGCGTGGACAGGAGGTGTGGATCCCCCCATTGGAGATGTGGAATACCTGACAGCCTTCAGGA CAGTAGTGATGCCCATTGCCCACGAGTTCTCACCCGACGTCGTTCTAGTCTCCGCTGGGTTTGATGCTGT TGAAGGACATCTGTCTCCACTGGGTGGCTATTCTGTCACCGCCAGATGTTTTGGCCACTTGACCAGGCAG CTCATGACACTGGCAGGGGGCCGGGTGGTGCTGGCCCTGGAGGGAGGTCATGACTTGACCGCCATCTGTG ATGCTTCTGAGGCCTGTGTCTCGGCTCTGCTCAGCGTGGAGCTGCAGCCCTTGGATGAAGCAGTCTTGCA GCAAAAGCCCAGCGTCAATGCAGTTGCCACACTAGAGAAAGTCATCGAGATCCAGAGCAAACACTGGAGC TGTGTACAGAGGTTTGCCACTGGTCTGGGCTGCTCGCTGCGGGAGGCTCAGACGGGTGAGAAAGAGGAGG CCGAGACTGTGAGCGCCATGGCCCTGCTTTCCATGGGGGCTGAGCAGGCCCAGGCTGCTGCCACTCAAGA GCACAGCCCCCGGCCAGCCGAGGAGCCCATGGAGCAGGAGCCTGCCCTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_053450
Insert Size	3342 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_053450.1, NP_445902.1
RefSeq Size	3754 bp
RefSeq ORF	3342 bp
Locus ID	84580
Gene Summary	mouse homolog is involved in mediating nuclear receptor repression [RGD, Feb 2006]

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