Gli1 (NM_001191910) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Gli
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217480 representing NM_001191910 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCAATCCAATGACTTCACCACAAGTCAATAGCTATGGTGAGCCATGCTGTCTCCGACCCCTCCCCA GCCAAGGGGTCCCCAGCATGGGAACAGAAGGACTTTCTGGTCTGCCCTTTTGCCACCAAGCCAATTTTAT GTCAGGGTCCCAGGGTTATGGGTCTGCCAGAGAGACCAGCAGCTGCACTGAGGGGTCACTCTTTCCTCCT CCTCGGAGTTCAGTCAAACTGACTAAGAAGCGGGCTCTTTCCATCTCGCCTCTCTCTGACGCCAGCCTCG ACCTGCAAACCGTAATCCGTACCTCACCCAGCTCCCTCGTGGCTTTCATCAACTCTCGTTGTACATCTCC AGGCGGTTCCTACGGCCATCTCTCCATTGGCACCATGAGCCCTTCTTTAGGATTCCCACCTCAGATGAGT CACCAGAAAGGAACTTCACCTCCCTATGGAGTCCAGCCCTGTGTTCCACATGATTCTACTCAGGGTTCAA TGATGCTTCACCCCCAGTCCCGAGGACCACGTGCAACCTGCCAGCTGAAGTCAGAGCTGGACATCATGGT TGGCAAGTGCCCAGAGGAACCTTTGGAAGGGGACATGTCTAGCCCCAACTCCACGAGCACACAGGATCAC CTGCTGGGGATGCTGGATGGACGGGAGGACCTGGAGAGAGAGGAGAAACCTGAGCCTGAGTCTGTGTATG AGACGGACTGCCGCTGGGATGGTTGCAGCCAGGAGTTCGATTCCCAAGAACAGCTGGTGCACCACATCAA CAGTGAGCACATCCACGGGGAGCGGAAGGAGTTCGTGTGCCACTGGGGAGGCTGCTCCAGGGAGCTGAGG CCCTTCAAGGCCCAGTACATGCTGGTGGTGCACATGCGCAGACACACGGGCGAGAAGCCACACAAGTGCA CGTTTGAAGGCTGTCGGAAGTCCTATTCACGCCTCGAAAACCTGAAGACACACCTTCGGTCGCACACCGG TGAGAAGCCTTATATGTGTGAGCAGGAAGGCTGCAGCAAGGCCTTCAGCAACGCCAGTGACCGTGCCAAG CACCAGAATAGGACCCACTCCAATGAGAAGCCATATGTGTGCAAACTCCCCGGCTGCACCAAGCGCTACA CAGATCCCAGCTCGCTCCGCAAACATGTCAAGACTGTGCACGGTCCGGATGCCCACGTGACCAAACGACA TCGAGGGGATGGCCCCTTACCACGGGCACAGCCCCTCTCCACAGTGGAGCCCAAGCGAGAAAGGGAAGGA GGATCCGGCAGGGAAGAGAGCAGACTGACTGTGCCGGAGGGCACCATGCCTCAGCAGAGCCCTGGAGCAC AATCCTCCTGCAGCAGTGACCACTCCCCAGCAGGCAGCGCGGCCAACACGGACAGCGGCGTGGAGATGGC CGGCAACGCAGGGGGCAGCACTGAGGACTTGTCCAGCTTGGATGAAGGACCTTGCGTTGCGGCTACCGGA CTCTCCACCCTTCGCCGCCTTGAGAACCTCAGGCTGGATCAGCTGCATCAGCTCCGGCCAATAGGGTCCC GGGGTCTCAAACTGCCCAGCTTAACCCACGCTGGCGCACCTGTGTCTCGACGTCTGGGCCCACCGGTCTC CCTTGACCGCCGCAGCAGCAGCTCCAGCAGCATTAGCTCCGCGTACACAGTCAGCCGCCGGTCCTCGCTG GCATCCCCGTTCCCGCCGGGATCCCCACCAGAGAACGGGGCGTCGTCACTACCTGGCCTCACACCTGCCC AGCACTACATGCTCCGGGCAAGATATGCTTCAGCCAGGGGGAGTGGCACCCCACCCACTGCAGCACACAG CCTGGATCGGATCGGGGGTCTTCCTGCACCTCCTTGGAGAAGCCGAGCTGAGTACCCAGGATACAACCCC AATGCAGGGGTCACGAGGAGGGCCAGTGACCCAGCCCGGGCTGCTGACCACCCGGCTCCAGCTAGAGTAC AACGGTTCAAGAGCTTGGGGTGTGTACACACGCCTCCTAGTGTGGCAACAGGACGGAACTTCGATCCCCA CCTCCCTACCTCTGTCTATTCGCCACAGCCCCCCAGCATCACCGAAAATGTTGCTATGGATACTAGAGGG CTACAGGGGGAGCTAGAGGTTGGAACTTCCATGATGGGCAATGATCTGAACCCATACATGGACTTTTCTT CTGCTGACACTCTGGGATATGGGGGGCCTGAGGGGACGGCGGCTGAGTCTTATGGATCTAAGGGTCCAGG TTCCCTGCCTCTTGGGCCTGGTCCACCAACCAACTATGGCCCTGGCCACTGTGCCCAGCAGGTCTCCTAT CCTGATCCCACCCCAGAAACATGGGGTGAGTTTCCTTCACATGCTGGGGTGTACCCTGGCACTAAGGCTC TAGGTACAGCCTATAACCAGTGTCCTCGACTTGAGCATTATGGACAAGTGCAAGTAAAACCAGAACAAGG GTGCCCAGTGGGGTCTGACTCCACCGGGTTGGCACCCTGCCTCAATGCCCACCCCAGTGAAGGGTCCCCA GACCCACAGCCTCTGTTTTCACATCACTCCCAGCTCCCTCCACCCCAGTATCTCCAGTCAGGTCCCTACC CTCAGCCTCCCCATGACTGTCTCTCGTTAGAATCCAGGCCTGGCCTCAATTTCAACCCCTCCTCTCATTC CACAGGACAGCTCAAAGCTCAGCTCCTGTGTAATTACGTTCAGTCGCAGCAGGAATTGTTGTGGGAGGGC AGAAGCCGGGGAGGGATCCCCAACCAGGAACTTCCATATCAGAGCCCCAAGTTTCTGGGAGGTTCTCAAG TTAGTCAGAGCCCCACCAAGGTCCCAGCAGCAGCAGCAGCAGCTACGTTTGGATCTGGCTTTGCATCACC TGCTTTGGCCAATCACAAATCAGTCTCCTATCCTGCCCCTTCACCGTGCCATGAAACTTTCACCATGGGA GTAAACAGGCCTCCCCATAGGCCAGCAGCACCACCCCGGCTTCTGCCCCCGCTACCACCTTGCTATGGGC CCCTCAAGGTGGGAGATGCCAACCCCAGCTGTGGGCATCCTGAAGTGGGCAGGTTAGGAGCAGGCCCTGC CTTGTACCCTCCCCCTGAAGGGCAGGTGTGTAACCCTCTGGACTCTCTTGACCTGGACAACACTCAGCTG GACTTTGTGGCTATCCTAGATGAAGCTCAAGGGCTGAGCCCTCCTCCTTCCCACGAGCAAGGGGACAGCT CTAAAAACACCCCACCTCCCTCCGGGCCCCCCAACATGGCAGTCGGTAACATGAGTGTCTTACTGGGGTC TCTGCCTGGAGAGACGCAGTTCCTCAACTCTAGTGCCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001191910
Insert Size	3330 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001191910.1, NP_001178839.1
RefSeq Size	3501 bp
RefSeq ORF	3330 bp
Locus ID	140589
Gene Summary	mouse homolog is a zinc finger transcription factor and oncogene; involved in development [RGD, Feb 2006]

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