Cp (NM_001270961) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	CERP
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217464 representing NM_001270961 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGTTTTTGCTGCTTAGTGCACTTTTATTTTTGCATAGTTCCTTAGCTTGGACAAGAGAAAAGCATT ATTACATCGGAATTACTGAAGCAGTTTGGGACTATGCTTCTGGCAGTGAAGAAAAGGAACTTATTTCAGT TGACACGGAACAGTCCAATTTCTATCTTCGAAATGGTCCAGATCGTATTGGAAGAAAGTATAAGAAGGCC CTTTATTCTGAGTACACAGATGGCACCTTTACGAAGACTATAGACAAACCAGCCTGGCTAGGGCTTTTAG GCCCTGTCATCAAAGCTGAAGTTGGAGACAAAGTTTCTGTTCACGTAAAGAACTTTGCCTCTAGGCCCTA CACTTTTCATGCTCATGGGGTAACTTACACCAAGGCGAACGAGGGGGCCATCTACCCTGACAACACCACT GATTTTCAAAGAGCCGATGACAAACTGTTTCCTGGACAGCAGTATTTGTACGTGCTGCGTGCCAATGAGC CAAGTCCTGGCGAGGGAGACAGCAATTGTGTGACCAGGATTTACCACTCTCATGTGGATGCTCCAAAAGA TATTGCATCAGGACTCATAGGACCGTTGATACTCTGTAAAAAAGGTTCTCTGCATAAGGAAAAAGAGGAA AATATTGACCAAGAATTCGTACTGATGTTCTCTGTGGTGGATGAAAATCTCAGCTGGTATCTAGAAGATA ACATCAAAACCTTCTGCTCGGAACCAGAGAAAGTCGATAAAGACAATGAAGACTTCCAGGAAAGCAACAG GATGTACTCTATAAATGGATATACATTTGGAAGCCTCCCAGGGCTCTCGATGTGTGCAGAAGACAGAGTG AAGTGGTACCTTTTTGGGATGGGGAATGAAGTTGACGTGCATTCAGCGCTCTTTCATGGTCAAGCCCTGA CCAGCAAGAACTATCATACTGATATAATCAACCTGTTCCCTGCCACTCTAATTGATGTTTCTATGGTGGC CCAGAATCCTGGAGTCTGGATGCTCAGTTGCCAGAACCTGAACCATCTGAAAGCTGGTTTGCAGGCCTTT TTCCAGGTTCGTGACTGCAACAAGCCCTCACCGGACGACGATATCCAAGACAGACATGTGAGACATTATT ACATCGCTGCCGAGGAGACCATTTGGGACTATGCTCCGTCTGGGACAGACACCTTCACTGGAGAGAACTT AACCAGTCTGGGAAGTGATTCAAGGGTATTTTTTGAGCAAGGTGCTACAAGAATTGGTGGCTCTTATAAA AAATTGGTTTATCGTGAGTACACAGATGATTCCTTCACAAACCGGAAGCAAAGAGGCCCTGATGAGGAAC ATCTTGGAATCCTTGGTCCTGTCATTTGGGCAGAAGTAGGAGACATCATTAGAGTCACCTTTCATAACAA AGGACAATTTCCTCTCAGCATTCAGCCAATGGGGGTAAGATTCACCAAGGAAAATGAGGGAACATACTAT GGCCCAGATGGCCGTTCCTCAAAGCAAGCCTCCCATGTGGCTCCCAAAGAAACCTTTACGTATGAATGGA CTGTCCCCAAAGAAATGGGACCCACTTATGCAGATCCTGTGTGCCTATCTAAGATGTATTATTCTGGAGT TGACCTCACCAAAGATATATTTACTGGGCTTATTGGGCCAATGAAAATATGCAAGAAAGGCAGCTTACTT GCAGATGGGAGACAGAAAGATGTAGACAAGGAGTTCTACTTGTTTGCAACAGTGTTTGATGAGAATGAGA GTTTACTCTTGGATGATAATATCAGAATGTTCACAACTGCACCTGAGAATGTGGACAAGGAAGATGAAGA CTTTCAGGAGTCCAACAAGATGCACTCCATGAATGGATTCATGTATGGCAATCTGCCTGGCCTCAATATG TGTCTAGGAGAATCCATCGTGTGGTATTTGTTCAGCGCTGGAAATGAGGCAGACGTGCATGGGATATACT TTTCAGGAAATACCTATCTGTCCAAAGGAGAAAGAAGAGACACTGCAAATCTATTTCCTCATAAAAGTCT CACCCTTCTCATGACACCTGACACAGAAGGGTCTTTTGATGTTGAGTGTCTTACAACAGATCACTACACC GGTGGCATGAAGCAAAAGTACACTGTGAACCAGTGCAAGGGGCAGTTTGAAGATGTCACTCTCTACCAGG GAGAAAGGACCTACTATATTGCAGCAGTGGAGGTGGAATGGGATTATTCACCAAGCAGGGACTGGGAAAT GGAGCTGCACCATTTGCAAGAGCAAAATGTTTCAAATGCATTTTTGGATAAGGAAGAGTTTTTCATAGGC TCAAAGTACAAGAAGGTTGTGTATCGAGAGTTTACTGACAGCACATTCAGAGAACAGGTGAAGAGAAGAG CTGAAGAGGAGCACTTGGGCATCCTCGGTCCACTGATTCATGCAGATGTTGGAGACAAAGTTAAAGTTGT CTTTAAAAATATGGCAAGCAGGCCATATTCAATACATGCCCACGGAGTGAAAACAAAGAGTTCTACAGTT GCTCCAACGTTACCAGGTGAAGTTCGCACTTATATATGGCAAATTCCAGAAAGATCAGGTGCTGGAACGG AGGATTCACCTTGTATCCCATGGGCTTATTACTCAACCGTGGATCGAGTTAAGGATCTCTATAGTGGGCT AATAGGCCCATTGATTGTTTGTCGGAAATCTTATGTGAAAGTATTCAATCCTAAAAAGAAAATGGAGTTT TCCCTTTTGTTTCTAGTATTTGATGAGAATGAATCTTGGTACTTAGATGATAACATCAATACATACTCTG ATCACCCTGAGAAAGTAAACAAAGACAACGAGGAATTCATAGAAAGCAATAAAATGCATGCTATCAATGG GAAAATGTTCGGAAACCTACAAGGTCTCACGATGCACGTGGGAGACGAGGTCAACTGGTATGTGATGGCT ATGGGCAATGAAATAGACCTGCACACTGTACACTTCCACGGCCACAGCTTCCAATACAAGCACAGGGGAA TTCATAGTTCTGATGTCTTTGACCTTTTTCCTGGAACATACCAAACCCTAGAGATGTTTCCCCAAACGCC TGGAACCTGGTTACTCCACTGCCATGTGACTGACCATATTCATGCGGGGATGGTAACTACCTACACTGTT TTACCAAATCAAGCATCGTCTCAGAGCTACAGGATGACCTGGAACATACTCTATACACTGTTAATCAGCA TGACTACTTTATTCCAAATATCTACCAAGGAGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001270961
Insert Size	3255 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001270961.1, NP_001257890.1
RefSeq Size	3607 bp
RefSeq ORF	3255 bp
Locus ID	24268
Gene Summary	copper-containing ferroxidase that promotes iron incorporation into transferrin; plays a role in iron metabolism and homeostatis [RGD, Feb 2006] Transcript Variant: This variant (2) lacks an exon and contains two alternate 3' exons compared to variant 1. The resulting protein (isoform 2), which is longer and has a distinct C-terminus compared to isoform 1, is a glycosylphosphatidylinositol-anchored form. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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