Crim1 (NM_001169103) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217430 representing NM_001169103 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTACTTGGTGGCGGGGGGCAGGGGGCTGGCCGGCTGCGGGCACCTCTCGGTCTCGCTGCTGGGGCTGC TGCTGCTGCTGGCGCGCTCAGGCACCCGGGCGCTGGTCTGCCTGCCCTGTGACGAGTCCAAGTGCGAGGA GCCTCGGAGCTGCCCGGGAAGCATCGTGCAGGGCGTCTGCGGCTGCTGCTACATGTGCGCCCGCCAGAGG AACGAGAGCTGCGGTGGAGCCTATGGGCTCCATGGAGCCTGCGACCGGGGGCTGCGCTGTGTCATCCGCC CCCCGCTCAATGGCGACTCCATCACCGAGTATGAAGTGGGCGTCTGCGAAGACGAGGACTGGGACGACGA CCAGCTGCTCGGTTTTGAGCCCTGCAATGAGAACCTCATCTCGGGCTGCAATATCATCAACGGGAGATGC GAATGCAGTACCATCCGAACCTGCAACAACCCCTTTGAGTTCCCAAGGAAGGACATGTGCCTTTCGGCCT TAAAGAGGATCGAAGAAGAGAAGCCAGATTGCACCAAGGCCCGCTGTGAAGTGCAGTTCTCTCCACGTTG CCCTGAAGATTCCATTCTGATTGAGGGCTATGCTCCCCCCGGGGAGTGTTGTCCTTTACCCAGCCGCTGC GTGTGTGACCCTGCGGGCTGTCTGCGCAAAGTCTGCCAGCCGGGATACCTGAACATTCTAGTGTCCAAAG CCTCAGGGAAGCCGGGAGAGTGCTGTGACCTCTATGAGTGTAAACCAGTTTTCAGCGTGGACTGCAGCAC CGTGGAGTGCCCCCCTGTCCAGCAGGCCGTGTGCCCCCTGGACAGCTACGAAACGCAAGTGCGGCTCACA GCGGATGGCTGCTGTACCCTGCCAGCAAGATGCGAGTGTCTCTCTGGCTTATGTGCTTTTCCCGTTTGTG AGGTGGGATCTACTCCCCGAATAGTCTCTCGTGGAGATGGGACACCTGGAAAGTGCTGTGATGTCTTTGA ATGTGTTAATGAAACAAAGCCAGCCTGCGTGTTCAACAGTGTGGAGTATTACGATGGAGACATGTTTCGA ATGGACAACTGTCGGTTCTGTCGGTGCCAAGGGGGCGTCTCCATCTGCTTCACCGCCCAGTGTGGGGAAC TGAACTGCGAAAGATACTATGTGCCTGAGGGGGAGTGTTGCCCCGTGTGTGAAGATCCCATCTATCCTTT CAACAACCCCGCCGGCTGCTACGCCAACGGCCAGATCCGCGCCCACGGGGACCGGTGGCGGGAAGACGAC TGCACCTTCTGCCAGTGTATCAATGGAGAACCCCACTGCGTGGCCACCGCCTGCGGGCAGAGCTGCATGC ACCCCGTGAAAGTGCCTGGGGAGTGTTGCCCCGTGTGTGAAGAACCAACCTACATCACAATTGATCCACC TGCGTGCGGGGAGTTGTCAAACTGTACTCTGAAAGAGAAGGACTGCGTTTACGGCTTCAAACTGGATCAC AATGGCTGTCGAACCTGTCAGTGCAAAAACAGGGAGGAACGATGCTTAGCCCTTAAAAGGGCCTGCACTT TGGACTGTCCCTTCGGCTTCCTCACCGATGTCCACAACTGTGAGCTCTGTCAGTGCCGCCCACGGCCCAA GAAATGCAGACCAACAATATGCGACAAGTTTTGTCCTCTTGGATTCCTGAGGAACAAGCACGGCTGTGAC ATCTGTCGCTGTAAGAAGTGTCCAGAGCTTCCATGCAGCAAGGTCTGCCCCTTGGGCTTCCAGGAGGACA GTCACGGCTGTCTCATCTGCAAGTGCAGAGAGGTCCCTACTTCAGCCGGGCCACCTGTCCTGTCAGGCAC ATGTCTGTCCATGGATGGTCATCACCATAAAAACGAGGAGAGCTGGCATGATGGCTGCCGGGAATGCTAC TGTCACAATGGGAAGGAAATGTGTGCCCTCATCACCTGCCCAGTGCCTGCCTGCGGCAACCCCACCATCC GGCCCGGACAGTGCTGCCCATCCTGCGCAGATGACTTCGTGGTGCAGAAGCCCGAGCTCAGCACGCCTTC CATTTGCCACGCCCCTGGAGGAGAGTACTTTGTGGAAGGGGAAACGTGGAACATTGACTCCTGTACGCAG TGCACCTGTCACAGTGGCCGAGTGCTGTGTGAGACGGAGGTGTGCCCACCACTGCTCTGCCAGAACCCCT CACGCACCCAGGACTCCTGCTGCCCACAGTGTACAGAGGACCCTCTTCAGCCTTCCACATCCCATAATGA GAGCGTGCCTAGCTACTGCAGGAATGATGAAGGGGACATCTTCCTGGCGGCCGAGTCCTGGAAGCCTGAC GTCTGCACCAGCTGCGTGTGCATGGATAGCTCCATTAGCTGCTACTCCGAGTCTTGCCCTTCGGTGGCCT GCGAACGGCCCGTTTTGAGGAAAGGCCAGTGTTGCCCCTACTGTCTAGAAGACACGATTCCAAAGAAAGT GGTGTGTCACTTCAGTGGGAAGACCTACGCTGACGAGGAGCGGTGGGACATCGACAGCTGCACACATTGC TACTGCCTGCAAGGCCAGACCCTCTGCTCGACTGTCAGCTGCCCACCATTACCCTGTGCCGAGCCCATCA AGGTGGAAGGGAGTTGCTGCCCGATGTGCCCAGAAATGTATGTGCCAGAGCCAACCAATGTGCCCATTGA GAAGAAGAATCATCGCGGCGAGATTGACCTGGAGGTTCCCATGTGGCCCACCCCAAGTGAAAATGACATC ATCCACCTCCCTAGAGATATGGGTCACCTCCAGGTAGATTACAGAGACAATAACAGGCTGCATCCAAGTG AAGACTCATCATTGGACTCCATTGTCCCCGTTGTGGTTCCCATAATCATATGCCTGTCCATCATAATAGC ATTCCTGCTCATCAACCAGAAGAAACAGTGGATACCGCTGCTGTGCTGGTACCGGACACCAACCAAGCCT TCTTCCTTGAATAATCAGCTGGTATCGGTGGACTGCAAGAAAGGCACTCGGGTCCAGGTGGACGGTTCCC AGAGAATGCTAAGAATCGCTGAACCAGATGCACGATTCAGTGGCTTCTACAGCATGCAGAAACAGAACCA TCTACAGGCAGACAATTTCTACCAAACGGTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001169103
Insert Size	3114 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001169103.1, NP_001162574.1
RefSeq Size	4076 bp
RefSeq ORF	3114 bp
Locus ID	298744

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