Cep120 (NM_001191697) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Ccdc100; RGD1565619
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217389 representing NM_001191697 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTCCCCAAGTCCGACCAGCTGCTCATCGTCGTATCCATCCTCGAAGGTCGCCATTTCCCCAAACGTC CAAAGCATATGCTTGTGGTGGAAGCAAAGTTTGATGGGGAGCAGTTGGCCACTGATCCTGTCGACCATAC TGACCAGCCAGAATTTGCTACTGAGTTAGCCTGGGAGATCGACAGGAAAGTCCTTCATCAGCACAGGTTA CAGCGCACTCCCATCAAACTGCAGTGCTTTGCCTTGGATCCTCAGACTTCTGCCAAGGAAACCGTAGGCT ACATTGTTTTGGATTTAAGAACAGCTCAAGAAACAAAGCAGGCACCCAAATGGTACCAGTTGCTGAGTAA CAAATACACCAAATTCAAGTCCGAGGTACAGATAAGCATCACTTTGGAAACAGATACAAAGGCACAGGTG GATAGTTTTAAAGCAAAAGGAGCCCCACCTCGGGATGGAAAAGTTCCTGCTAGCCTGTTAGGAGTTGACC CTAAGGACATCGTGGCAGTGTTGAATGAGGAGGGAGGCTACCATCAGATTGGACCAGCAGAGTGTTGTAC TGACTCCTTTACTCTCTCAGTGACCATAGCATTTGCCACCCAGTTGGAACAGTTAATTCCATGTACCATG AAGCTTCCAGAAAGGCAGCCTGAGTTTTTCTTTTACTATTCTTTATTGGGAAATGATGTTACAAATGAGC CGTTTAGTGATTTGATCAACCCGAACTTTGAGCCGGAGAGGGCGTCTGTCCGCATACGAAGCAGCGTGGA GGTCCTTCGTGTGTACCTGGCTCTTCACTCGAAACTGCAGATCCATCTCTGCTGTGGAGACCAGTCACTC GGCAGTACAGAAATACCTCTAAGTGGATTACTGAAGAAGGGCAGCACAGAGATTAACCAGCATCCAGTCA CAGTCGAGGGTGCGTTTACCCTTGACCCTCCAAACCGAGCCAAGCAGAAGCTCGCTCCTGTTCCTGTCGA GCTGGCCCCAACTGTGGGAGTGTCAGTGGCTCTGCAGAGAGAAGGCATCGGCTCCCAGTCTTTAATTGAA TTAAAGACCCAGAATGGACATGAGCCTGAGCACTCACAGAAGAGAGTTTTAACCCCCATAAAGGAGAAGA CACTCACTGGGCCAAAATCACCAAGGGTGTCCCCTGCCCCACCTCAAAACCAGACACCTCCAACCAAGGA CGATGCCACAGAAAGTGAAGTGGAGAGCCTGCAGTACGATAAGGAGCCGAAGCAGGTGGTGAAGGGCACT GGTTCCATACCTGCTTCGCTGGCCCAGCCAGAGGCGACTGGCAACCCATCAGAAGTGGTGACATCGGGGC AGAAGATTGCTGTTCCAGCAACATCACACCACTTCTGCTTTTCAATAGACTTGAGAAGCATCCATGGCTT GGAAATTAGTTTTCCAGTCAACTGCATATTGAGGTACTCCTACCCATTCTTCGGCAGTGCAGCTCCTATT ATGACTAACCCTCCTGTAGAAGTTCGGAAGAACATGGAAGTTTTTCTTCCTCAGTCTTACTGTGCATTTG ATTTTGCAACTATGCCTCACCAGCTACAAGACACCTTCCTAAGGATTCCACTGCTTGTAGAACTATGGCA CAAGGATAAAATGAGTAAAGACTTACTCCTTGGGATTGCAAGAATTCAGCTTTCTAACATCTTGTCTTCA GAAAAGACCCGGTTTCTAGGTGCTAATGGTGAACAGTGCTGGCGACAGACTTACAGTGAGAAAGTGCCTG TCATAGCGGCCCAGGGGTCAAATAACAGGATAGTAGACCTTTCTTACACAATGACTCTGGAGGACTATGG CCTGGTGAAAATGCGTGAGATCTTTGTATCTGAGTCATCTCAGGGCATGTCTGCTGTGGGTCAGAGGGCG TCTCCTCCTCCTCCTCCTCCAGCTCCTTGCCCTTCTGAGATCCAGGTGGAGCCCCGGGAGACCTTAGAGT ACAAGGCTGCACTGGAGCTGGAGATGTGGAAAGAGATGCAGGAGGACATATTTGAAAGTCAGCTGAAGCA GAAAGAACTGGCTCACATGCAAGCTCTTGCAGAAGAGTGGAAAAAAAGGGACCGGGAAAGAGAGTCACTA GTGAAGAAAAAGGTAGCTGAGTACAGTATTCTAGAAGGCAAACTTCAACAAGCCCTAACGGAGCTCGAGA GCCGAGAGCAGCAGCTTGTCACTGCAGAGACAGAGCTTCAAAGAGAGAGAAAGGAACTGCAGTTAGAACG AGAGCGGAACCTTCAAGAGCTGCAGGACTCTGTCCGAAGGGCCAGAGACGACTGCGTGCACCAAGTGGAG CTGGAGAGGCTGAAGCTGAAGCAGCTGGAAGAGGACAAACACCGCCTTCAGCAGCAGCTTAATGATGCTG AAAATAAGTATAAGATTTTGGAAAAAGATTTTCAGCAGTTCAAGGACCAACAAAACAACAAACCAGAAAT ACGGCTGCAGTCAGAAATAAACCTCCTCACCTTGGAAAAGGTTGAACTTGAAAGAAAGTTGGAGTCTGCA ACCAAGTCTAAACTGCATTACAAGCAGCAGTGGGGCCGAGCTTTGAAAGAACTTGCCAGACTCAAGCAGA GGGAGCAAGAAAGCCAAATGGCTCGCCTTAAAAAACAGCAGGAGGAGTTGGAGCAGATGCGGCTGCGTTA CCTGGCTGCTGAGGAGAAAGACACAGTGAAAACTGAGCAACAAGAACTGCTGGACATAAGGAACGAACTG AACAGGTTACGGCAGCAAGAACAGAAACAGTACCAGGACTACAAAGAGATTGCAAGTGGGAAACAGGGCA GCCCTCGGGGCTCTGGGCTGGAAGAAGGCCTGGATGACTACTTGACACGTCTGATAGAAGAACGGGATAC TTTGATGAGAACGGGTGTCTATAACCATGAGGACCGCATAATAAGTGAACTCGACCGGCAGATCAGAGAG GTTTTGACAAAAAACAGTGCCAGTAATTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001191697
Insert Size	2970 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001191697.1, NP_001178626.1
RefSeq Size	4586 bp
RefSeq ORF	2970 bp
Locus ID	307302

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