Grik5 (NM_031508) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	GluK5; iGlu5
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217383 representing NM_031508 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGGCTGAGCTGCTGCTGCTGCTGATAGTCGCCTTCGCCAACCCCAGCTGCCAGGTGCTGTCATCAC TGCGCATGGCTGCAATCCTGGACGACCAGACCGTGTGTGGCCGTGGTGAGCGTCTGGCCCTGGCCCTGGC CCGAGAGCAGATCAATGGGATCATCGAGGTCCCAGCCAAGGCCCGAGTGGAAGTAGACATCTTTGAGCTG CAACGGGACAGCCAGTACGAGACTACGGACACCATGTGTCAGATCCTGCCCAAGGGGGTTGTGTCTGTCT TGGGACCCTCCTCCAGCCCAGCTTCTGCCTCCACCGTGAGCCATATCTGTGGGGAGAAGGAGATTCCCCA CATCAAGGTAGGCCCTGAGGAGACACCCCGCCTTCAGTACCTCCGCTTTGCATCTGTCAGCCTGTATCCC AGTAATGAAGATGTCAGCCTGGCAGTCTCCCGGATCCTCAAGTCCTTTAACTACCCCTCCGCTAGCCTCA TCTGCGCCAAAGCTGAGTGCCTGCTGCGGTTAGAAGAACTGGTGCGAGGCTTCCTCATCTCCAAGGAGAC ACTGTCCGTGAGGATGCTTGATGACAGCCGGGACCCCACGCCGCTACTCAAGGAGATCCGAGATGACAAA GTGTCCACCATCATCATCGATGCCAACGCGTCCATCTCCCACCTTGTCCTCCGTAAGGCTTCGGAGCTGG GAATGACCTCAGCGTTTTACAAGTACATCCTCACCACCATGGACTTCCCCATCCTGCATCTGGACGGTAT CGTAGAGGACTCCTCCAACATCTTGGGCTTTTCCATGTTCAACACCTCCCATCCCTTCTACCCGGAGTTT GTGCGCAGCCTCAACATGTCCTGGAGGGAGAACTGCGAAGCCAGCACCTACCCTGGCCCTGCGCTGTCGG CAGCCCTGATGTTTGATGCCGTGCATGTGGTGGTAAGCGCTGTCCGAGAGCTGAACCGGAGCCAGGAGAT TGGTGTCAAGCCACTGGCTTGCACTTCGGCCAACATCTGGCCCCATGGGACCAGCCTCATGAACTACCTG CGCATGGTAGAGTATGACGGGCTGACTGGGCGGGTTGAGTTCAACAGCAAAGGGCAGAGGACCAACTACA CACTGCGCATCCTGGAGAAGTCCCGCCAGGGCCACCGTGAGATAGGGGTGTGGTACTCTAACCGGACCCT GGCCATGAATGCTACCACCCTGGACATCAACCTGTCACAGACTCTAGCCAACAAGACTCTGGTGGTCACA ACAATCCTGGAGAACCCGTATGTCATGCGCCGGCCCAACTTCCAGGCCCTGTCAGGGAACGAGCGCTTTG AGGGCTTCTGCGTGGACATGCTTCGGGAGCTGGCCGAGCTGCTGCGCTTCCGGTATCGCCTGCGGTTGGT GGAGGACGGACTGTACGGGGCACCTGAGCCCAATGGTTCCTGGACAGGAATGGTTGGCGAGCTCATCAAC CGGAAGGCAGACCTGGCTGTGGCAGCCTTCACCATCACCGCGGAGAGAGAGAAGGTCATCGACTTCTCCA AGCCATTCATGACCCTGGGGATCAGCATCCTCTACAGGGTGCACATGGGCCGCAAGCCTGGCTACTTCTC CTTCCTGGACCCTTTCTCCCCTGCCGTGTGGCTCTTCATGCTTCTTGCCTACCTGGCTGTCAGCTGTGTC TTGTTTCTGGCTGCCAGGCTGAGCCCTTATGAGTGGTATAACCCTCACCCGTGTCTTCGGGCGCGTCCCC ATATCCTGGAGAACCAGTACACGCTGGGTAACAGCCTTTGGTTCCCTGTGGGTGGCTTCATGCAGCAGGG CTCGGAGATCATGCCCCGGGCGCTGTCCACACGCTGTGTCAGCGGAGTCTGGTGGGCCTTCACCTTGATC ATCATCTCCTCCTACACGGCCAACCTGGCTGCCTTCCTCACGGTGCAGCGCATGGAGGTGCCGGTGGAGT CTGCAGACGACTTGGCGGATCAGACCAACATTGAGTACGGCACTATCCACGCTGGCTCCACCATGACCTT CTTTCAGAACTCACGGTACCAGACGTACCAGCGGATGTGGAATTACATGCAGTCGAAGCAGCCCAGCGTG TTCGTCAAGAGCACAGAGGAGGGGATCGCCCGTGTCCTCAACTCACGTTACGCCTTCCTGCTGGAGTCCA CCATGAACGAGTACCACAGGCGCCTCAATTGCAACCTCACCCAGATCGGGGGCCTCCTCGACACCAAGGG CTACGGCATCGGCATGCCGCTGGGCTCCCCATTCCGGGATGAGATCACGCTGGCCATCCTGCAGCTCCAG GAGAACAACAGGCTGGAGATCCTGAAGCGCAAGTGGTGGGAGGGCGGCCGGTGCCCCAAGGAGGAGGACC ACAGGGCCAAAGGTTTGGGCATGGAGAACATTGGCGGTATTTTTGTCGTGCTGATCTGTGGCCTCATCAT TGCTGTCTTCGTGGCGGTCATGGAATTCATCTGGTCCACGCGGAGGTCAGCGGAGTCTGAGGAGGTGTCG GTGTGCCAGGAGATGCTGCAGGAGCTGCGCCACGCCGTGTCTTGCCGCAAGACCTCGCGTTCCCGCCGGC GCCGGCGCCCTGGCGGCCCGAGCCGGGCCCTGCTGTCGCTGCGCGCGGTCCGCGAGATGCGACTCAGCAA CGGCAAGCTCTACTCGGCCGGCGCGGGCGGGGACGCGGGCGCGCACGGGGGTCCGCAGCGCCTCCTGGAC GACCCCGGGCCTCCTGGGGGACCCCGGCCCCAGGCTCCCACGCCCTGCACGCACGTGCGCGTCTGCCAGG AGTGCAGGCGCATCCAGGCGCTGCGAGCTTCAGGGGCCGGGGCGCCCCCGCGTGGCCTGGGCACTCCGGC CGAAGCCACCAGCCCGCCTCGGCCGCGGCCTGGACCCACTGGACCCCGCGAGCTGACTGAGCATGAATGA ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA TTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-NotI
ACCN	NM_031508
Insert Size	2940 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_031508.2, NP_113696.1
RefSeq Size	3776 bp
RefSeq ORF	2940 bp
Locus ID	24407
UniProt ID	Q63273
Gene Summary	glutamate receptor subunit KA2; associates with subunits of the GluR5-7 family to form heteromeric kainate-preferring glutamate receptpors [RGD, Feb 2006]

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