Chrd (NM_057134) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217355 representing NM_057134 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGAGCCTCCCGGCCCCGCCGGCCCCTCGGCTGCTCCTCGGGCTGCTGCTGCTCGGCTCGCGGCCGG CCGGTGGCACTGGCCCTGAGGCCCCCGCACTGCCCATCCGTTCCGAGAAGGAGCCACTGCCTGTCCGGGG AGCGGCAGGCTGCTCCTTCGGCGGGAAGGTCTATGCCTTGGACGAGACGTGGCACCCGGACCTGGGGGAG CCTTTTGGGGTGATGCGCTGTGTGCTGTGCGCCTGCGAAGCGCCTCAGTGGGCTCGCCGTGGGAGGGGCC CTGGCAGGGTCAGCTGCAAGAACATCAAACCTCAGTGCCCCACCCTGGCCTGCAGGCAGCCGCGCCAGCT GCCAGGACACTGCTGCCAGACCTGCCCGCAGGAGCGCAGCAATCTAGATCCACAGCCGGCTGGCCTGGTC TTCGAGTATCCAAGGGACCCAGAGCATCGCAGTTATAGCGATCGAGGGGAACCCGGCACTGGGGAGCGGA CACGTGGTGATGGCCACACGGACTTTGTGGCGTTGCTGACTGGACCGAGGACGCAGGCGGTAGCTCGTGC TCGAGTCTCTTTGCTGCGCTCAAGTTTACGCTTCTCTATCTCCTACCAGCGGCTGGACCGTCCCAGCAGG GTTCGGTTCACAGACCCCACAGGCAACATCCTGTTTGAACACCCTGCTGCCCCCACCCAAGATGGCCTGG TCTGTGGGGTGTGGCGGGCAGTGCCTCGGCTGTCTGTGAGGCTCCTGAGGGCAGAGCAGCTGCGTGTTGC CCTTGTGACACCCACTCACCCTTCAGAAGAAGTCTGGGGGCCTCTCATTTGGCAGGGTGCTCTCACTGCA GAGACCTTCAGTGCCATCCTGACCCTGGAGGACCCACTGCAGCGGGGTGTCGGGGGCATAGCCCTGCTCA CTCTCAGTGACACAGAAGATGCCTTACATTTTTTGCTGCTCTTCCGGGGTCTGCTGGGAGGACTAGCTCA CGTCCCCTTGAAGCTTCAGATTCTCCACCAGGGACAGCTACTTCGAGAGCTCCAGGCCAATGCCTCAGCT CAGGAGCCAGGTTTTGCTGAGGTGCTGCCCAGCCTTACAGACCAAGAGATGGACTGGTTGGTGCTGGGGG AGCTGCAGATGGTCCTAGAGAAGATGGGTGGGCCAGAGCTACGCATCAGCGGGTATATCACGACCAGGCA GAGCTGTGATGTCCTTCAAAGCGTCCTCTGTGGGGCTGATGCCTTGATCCCAGTCCAGACGGGTGCTGCT GGCTCAGCCAGCTTCATACTGCTAGGAAATGGCTCCCTTATCTATCAGGTACAAGTGATAGGTACAGGTA GCGAGGTGGTGGCCATGACGCTGGAGACCAAGCCTCAGCGGAAGAACCAGCGCACTGTCCTGTGCCACAT GGCTGGACTCCAGCTGGGAGGACACATGGCTGTGGGTGTCTGCTCTGGGCTGGGTGCCCGAGGGGCTCAC ATGCTGCTACAGAATGAGTTGTTCCTGAATATTGGTACCAAGGACTTCCCAGACGGAGAGCTTCGGGGGC ACGTGACTGCCCTATGCTACAGCGGGCACAGTGCCCACTACGACAGATTGCCTGTGCCTCTGGCAGGGGC ACTAGTACTGCCCCCTGTGCGGAGTCAGGCAGCAGGACATGCCTGGCTGTCCTTGGATACACATTGCCAC TTACACTATGAGGTTCTATTGGCTGGGCTTGGTGGCTCAGAGCAAGGAACTGTCACTGCTCATCTCCTCG GGCCTCCTGGGATGCCAGGGCCCCAGCGACTGCTGAAGGGATTCTATGGCTCAGAGGCTCAGGGTGTGGT AAAAGATCTAGAGCCCGTGCTGCTGCGGCACCTGACACAGGGCACCGCCTCCCTGCTTATCACCACCAAG AGTAACCCTAGAGGAGAGCTACGCGGGCAGGTGCACATTGCCAGTCAGTGTGAGGTGGGAGGCCTGCGCC TGGCCTCAGAAGGAGTGCGGATGTCCTTGGCTCCAAATGGAGAGGCAGCTACATCACCCATGCTGCCTGC TGGGCCCGGCCCTGAAGCCCCCGTACCAGCCAAACACGGCAGCTCTGGGAGACCCCGAGATCCTAACACA TGTTTCTTCGAGGGGCAGCAGCGTCCCCACGGGGCTCGCTGGGCACCCAACTATGACCCACTCTGCTCCC TCTGCACCTGCCAGAGACGGACAGTGATCTGTGATCCTGTAGTATGCCCACCACCAAGCTGTCCCCACCC GGTGCAGGCACTGGACCAGTGCTGTCCCGTGTGTCCAGAGAAACAACGCAGTAGAGACCTTCCCAGCCTA CCAAATCTGGAGCCAGGAGAGGGCTGCTATTTTGATGGTGACCGGAGCTGGAGGGCAGCGGGTACCCGAT GGCACCCCGTTGTGCCCCCCTTTGGCCTAATTAAGTGTGCTGTCTGCACCTGCAAGGGGGCCACCGGAGA GGTGCACTGTGAGAAGGTGCAGTGTCCTCGCCTCGCCTGTGCTCAGCCTGTCCGTGCCAACCCCACCGAC TGCTGCAAACAGTGCCCAGTAGGGTCAGGGACTCATGCCAAGCTGGGAGACCCCATGCAGGCTGATGGGC CTCGGGGTTGTCGCTTTGCTGGGCAGTGGTTCCCAGAGAATCAGAGCTGGCACCCATCAGTGCCCCCCTT TGGGGAGATGAGCTGTATTACCTGCAGATGTGGGGCAGGGGTACCCCACTGTGAGCGGGACGATTGCTCG CCGCCACTATCCTGCGGCTCAGGGAAGGAGAGTCGATGCTGTTCCCACTGTACAGCCCAAAGGTCCTCTG AGACTAGAACCCTTCCCGAGCTGGAGAAAGAAGCTCAACGCTCCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_057134
Insert Size	2847 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_057134.1, NP_476475.1
RefSeq Size	3267 bp
RefSeq ORF	2847 bp
Locus ID	117275
Gene Summary	secreted protein; human homolog binds TGF-beta and similar morphogenetic proteins; may be important for organogenesis and dorsal/ventral pattern specification of neural crest [RGD, Feb 2006]

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