Grin1 (NM_001270608) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	GluN1; NMDAR1; NR1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217311 representing NM_001270608 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCACCATGCACCTGCTGACATTCGCCCTGCTTTTTTCCTGCTCCTTCGCCCGCGCCGCCTGCGACC CCAAGATCGTCAACATCGGCGCGGTGCTGAGCACGCGCAAGCATGAACAGATGTTCCGCGAGGCAGTAAA CCAGGCCAATAAGCGACACGGCTCTTGGAAGATACAGCTCAACGCCACTTCTGTCACCCACAAGCCCAAC GCCATACAGATGGCCCTGTCAGTGTGTGAGGACCTCATCTCTAGCCAGGTCTACGCTATCCTAGTTAGCC ACCCGCCTACTCCCAACGACCACTTCACTCCCACCCCTGTCTCCTACACAGCTGGCTTCTACAGAATCCC TGTCCTGGGACTGACTACCCGAATGTCCATCTACTCTGACAAGAGTATCCACCTGAGTTTCCTTCGCACG GTGCCGCCCTACTCCCACCAGTCCAGCGTCTGGTTTGAGATGATGCGAGTCTACAACTGGAACCACATCA TCCTGCTGGTCAGCGACGACCACGAGGGACGGGCAGCGCAGAAGCGCTTGGAGACGTTGCTGGAGGAACG GGAGTCCAAGAGTAAAAAAAGGAACTATGAAAACCTCGACCAACTGTCCTATGACAACAAGCGCGGACCC AAGGCAGAGAAGGTGCTGCAGTTTGACCCAGGAACCAAGAATGTGACGGCTCTGCTGATGGAGGCCCGGG AACTGGAGGCCCGGGTCATCATCCTTTCTGCAAGCGAGGACGACGCTGCCACAGTGTACCGCGCAGCCGC AATGCTGAACATGACGGGCTCTGGGTACGTGTGGCTGGTCGGGGAACGCGAGATCTCTGGGAACGCCCTG CGCTACGCTCCTGATGGCATCATCGGACTTCAGCTCATCAATGGCAAGAATGAGTCAGCCCACATCAGTG ACGCCGTGGGCGTGGTGGCACAGGCAGTTCACGAACTCCTAGAGAAGGAGAATATCACTGACCCACCGCG GGGTTGCGTGGGCAACACCAACATCTGGAAGACAGGACCATTGTTCAAGAGGGTGCTGATGTCTTCTAAG TATGCGGACGGAGTGACTGGCCGTGTGGAATTCAATGAGGATGGGGACCGGAAGTTTGCCAACTATAGTA TCATGAACCTGCAGAACCGCAAGCTGGTGCAAGTGGGCATCTACAATGGTACCCATGTCATCCCAAATGA CAGGAAGATCATCTGGCCAGGAGGAGAGACAGAGAAACCTCGAGGATACCAGATGTCCACCAGACTAAAG ATAGTGACAATCCACCAAGAGCCCTTCGTGTACGTCAAGCCCACAATGAGTGATGGGACATGCAAAGAGG AGTTCACAGTCAATGGTGACCCAGTGAAGAAGGTGATCTGTACGGGGCCTAATGACACGTCCCCAGGCAG CCCACGCCACACAGTGCCCCAGTGCTGCTATGGCTTCTGCATAGACCTGCTCATCAAGCTGGCGCGGACC ATGAATTTTACCTATGAGGTGCACCTGGTGGCAGATGGCAAGTTTGGCACACAGGAGCGGGTAAACAACA GCAACAAAAAGGAGTGGAACGGAATGATGGGCGAGCTACTCAGTGGCCAAGCGGACATGATTGTGGCACC ACTGACCATCAACAATGAGCGTGCGCAGTACATAGAGTTCTCCAAGCCCTTCAAGTACCAGGGCCTGACC ATTTTGGTCAAGAAGGAGATTCCCAGGAGCACACTGGACTCATTTATGCAGCCTTTTCAGAGCACACTGT GGTTGCTAGTAGGACTGTCAGTTCATGTGGTGGCTGTGATGCTGTACCTGCTGGACCGCTTCAGTCCCTT TGGCCGATTCAAGGTGAACAGTGAGGAGGAGGAGGAAGATGCACTGACCCTGTCCTCTGCCATGTGGTTT TCCTGGGGCGTCCTGCTCAACTCCGGCATTGGGGAAGGTGCCCCCCGGAGTTTCTCTGCACGTATCCTAG GCATGGTGTGGGCTGGTTTCGCCATGATCATAGTGGCTTCCTACACTGCCAACTTGGCAGCTTTCCTGGT GCTGGATCGGCCTGAGGAGCGCATCACGGGCATCAATGACCCCAGGCTCAGAAACCCCTCAGACAAGTTC ATCTACGCAACTGTAAAGCAGAGCTCCGTGGACATCTACTTCCGGAGGCAGGTGGAGTTGAGTACCATGT ACCGGCACATGGAAAAACACAATTACGAGAGCGCAGCTGAGGCCATCCAGGCTGTGCGGGACAACAAGCT GCACGCCTTTATCTGGGACTCGGCCGTGCTGGAGTTTGAGGCTTCACAGAAGTGCGATCTGGTGACCACG GGTGAGCTGTTCTTCCGCTCAGGCTTTGGCATCGGCATGCGCAAGGACAGCCCCTGGAAGCAGAACGTTT CCCTGTCCATACTCAAGTCCCATGAGAATGGCTTCATGGAAGATCTGGATAAGACATGGGTTCGGTATCA GGAATGCGACTCCCGCAGCAATGCTCCTGCAACCCTCACTTTTGAGAACATGGCAGGGGTCTTCATGCTG GTGGCTGGAGGCATCGTAGCTGGGATTTTCCTCATTTTCATTGAGATCGCCTACAAGCGACACAAGGATG CCCGTAGGAAGCAGATGCAGCTGGCTTTTGCAGCCGTGAACGTGTGGAGGAAGAACCTGCAGCAGTACCA TCCCACTGATATCACGGGCCCGCTCAACCTCTCAGATCCCTCGGTCAGCACCGTGGTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001270608
Insert Size	2721 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001270608.1, NP_001257537.1
RefSeq Size	3876 bp
RefSeq ORF	2721 bp
Locus ID	24408
UniProt ID	P35439
Gene Summary	subunit of NMDA-preferring ionotropic glutamate receptors; may play a role in long term potentiation [RGD, Feb 2006] Transcript Variant: This variant (7) lacks an alternate in-frame exon and uses an alternate splice junction at the 5' end of the last exon compared to variant 1. The resulting isoform (4b) lacks an alternate internal segment and has a shorter and distinct C-terminus compared to isoform 1b.

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