Actn2 (NM_001170325) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217307 representing NM_001170325 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATCAGATAGAGCCCGGCGTGCAGTACAACTATGTCTACGACGAAGATGAGTACATGATCCAGGAGG AGGAGTGGGACCGCGACCTGCTCCTCGACCCGGCCTGGGAGAAGCAGCAGAGGAAGACTTTCACTGCCTG GTGCAACTCGCACCTGCGCAAAGCCGGCACCCAGATCGAGAACATAGAGGAGGATTTCAGGAATGGCCTC AAGCTCATGCTGCTTCTGGAAGTCATCTCAGGGGAAAGGCTTCCCAAACCTGACCGGGGCAAAATGCGGT TCCACAAGATTGCGAATGTCAACAAGGCTTTGGATTACATAGCCAGCAAGGGAGTGAAATTGGTATCTAT TGGGGCTGAAGAAATCGTCGATGGCAATGTGAAGATGACCCTGGGTATGATCTGGACCATCATCCTCCGC TTCGCCATTCAGGACATCTCAGTGGAAGAAACATCTGCCAAAGAGGGCCTGCTGCTATGGTGTCAGAGGA AGACAGCACCATACCGAAATGTGAACATCCAGAACTTCCACACCAGCTGGAAAGATGGCCTTGGACTCTG TGCCCTCATCCACAGACACCGGCCTGACCTCATCGACTACTCAAAGCTTAACAAGGATGACCCCATAGGA AACATTAACCTGGCCATGGAAATAGCAGAGAAGCACTTGGATATCCCTAAAATGTTGGATGCTGAAGACA TTGTCAACACTCCCAAACCCGATGAAAGAGCCATCATGACTTACGTTTCCTGCTTCTACCATGCTTTCGC GGGAGCGGAGCAGGCCGAGACGGCAGCTAACAGGATATGTAAGGTTCTTGCTGTGAATCAAGAGAATGAG AGGCTGATGGAAGAATATGAGAGGCTAGCGAGTGAGCTCTTGGAATGGATTCGACGCACAATCCCCTGGC TGGAGAACAGGACACCGGAGAAGACCATGCAAGCCATGCAGAAGAAGCTGGAGGACTTCCGGGACTATCG TCGTAAGCACAAGCCCCCAAAGGTGCAGGAGAAGTGCCAGCTGGAGATCAACTTCAACACCCTGCAGACC AAGCTTCGGATCAGCAACCGGCCTGCCTTCATGCCCTCCGAGGGCAAGATGGTGTCGGACATTGCTGGTG CCTGGCAGAGGTTGGAGCAGGCCGAGAAGGGCTATGAGGAATGGCTATTGAATGAGATTCGAAGACTGGA GCGCTTGGAGCACTTGGCTGAGAAGTTCAGGCAGAAGGCCTCCACCCATGAGACATGGGCTTACGGCAAA GAACAGATCTTGCTGCAGAAGGACTATGAGTCTGCGTCCCTGACGGAAGTTCGAGCCCTACTTCGGAAGC ACGAGGCCTTTGAGAGTGACCTGGCGGCACACCAGGATCGTGTGGAACAGATTGCAGCCATTGCTCAGGA GCTCAATGAACTGGACTACCACGATGCTGTGAACGTCAATGATCGTTGCCAGAAAATTTGCGACCAGTGG GATAGATTGGGGACGCTCACCCAGAAGAGGAGGGAGGCCTTGGAGAGAACCGAGAAGTTGCTAGAAACAA TTGACCAGCTGCACCTGGAGTTTGCCAAGAGAGCTGCGCCCTTCAACAACTGGATGGAGGGCGCCATGGA GGACCTGCAGGACATGTTCATCGTCCACAGCATTGAGGAGATCCAGAGTCTGATCACCGCACATGAGCAG TTCAAGGCCACGCTGCCCGAGGCCGATGGGGAGCGGCAGTCCATCCTAGCCATCCAGAATGAGGTGGAGA AGGTGATCCAGAGCTACAGTATCCGGATCAGCTCCAGCAACCCCTACAGCACTGTCACCATGGACGAGCT GCGCAACAAGTGGGACAAGGTGAAGCAGCTGGTGCCAGTCCGGGATCAGTCCCTGCAGGAGGAGCTGGCA CGCCAACATGCTAATGAGCGCCTACGGCGCCAGTTTGCTGCCCAGGCCAATGCCATCGGCCCCTGGATCC AGAACAAGATGGAGGAGATCGCTCGGAGCTCCATCCAGATCACAGGGGCCCTGGAGGACCAGATGAACCA GCTGAAGCAGTACGAGCACAACATCATCAACTACAAGAACAACATTGACAAGCTGGAGGGCGACCACCAG CTCATCCAGGAAGCCCTGGTCTTCGACAACAAGCACACCAACTACACCATGGAGCATATCCGTGTCGGAT GGGAGTTGCTGCTCACAACTATCGCCAGAACCATCAATGAGGTGGAGACCCAGATCCTGACAAGAGATGC CAAGGGCATCACCCAGGAGCAGATGAATGAGTTCAGAGCCTCCTTCAACCACTTTGACAGGAGGAAGAAT GGCCTGATGGATCATGAGGATTTCAGAGCCTGCCTCATTTCCATGGGCTATGACTTGGGTGAAGCTGAGT TTGCCCGAATTATGACTCTGGTTGACCCCAACGGACAAGGCACGGTCACCTTCCAGTCCTTCATTGACTT CATGACTAGAGAGACCGCAGACACGGACACTGCAGAACAGGTCATCGCCTCCTTCCGGATTCTGGCTTCT GATAAGCCTTACATCTTGGCAGAGGAACTTCGTCGAGAGCTGCCTCCGGATCAGGCCCAGTACTGCATCA AGAGAATGCCCCCATACTCAGGCCCAGGCAGTGTCCCCGGGGCCCTGGACTACACTGCCTTCTCCTCTGC CCTCTACGGGGAGAGCGACCTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001170325
Insert Size	2685 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001170325.1, NP_001163796.1
RefSeq Size	2943 bp
RefSeq ORF	2685 bp
Locus ID	291245

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