Epha5 (NM_024367) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	EHK-1; Els1; Els1.
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217305 representing NM_024367 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGGGCTCCGGGCCCCGCGGTGCGGGACGCCGACGGACCCAGGGCAGAGGTGGCGGCGGCGACACCC CCCGCGTCCCTGCCTCTCTGGCAGGCTGCTATTCCGCACCTCTAAAGGGGCCCCTCTGGACGTGCCTTCT CCTGTGTGCGGCGCTCCGGACCCTTTTGGCCAGCCCCAGTAACGAAGTGAATTTATTGGATTCGCGCACT GTCCTGGGAGACCTTGGATGGATTGCTTTTCCAAAGAATGGGTGGGAAGAGATTGGTGAAGTTGATGAAA ACTATGCCCCCATCCACACCTATCAAGTGTGCAAAGTTATGGAACAGAATCAGAATAATTGGCTGTTGAC CAGTTGGATCTCTAACGAAGGTGCTTCCAGAATTTTTATTGAACTCAAGTTTACTCTGAGGGATTGCAAC AGCCTTCCTGGAGGACTGGGGACTTGCAAGGAGACCTTTAACATGTATTATTTTGAGTCGGATGATGAGA ATGGGAGAAATATCAAAGAGAACCAGTACATCAAGATCGATACCATTGCTGCTGATGAGAGCTTCACCGA ACTTGACCTTGGAGACCGGGTCATGAAGCTGAATACGGAGGTCAGAGATGTAGGACCTCTGAGCAAAAAG GGATTTTATCTTGCTTTCCAAGATGTCGGTGCTTGCATCGCTCTGGTTTCTGTCCGTGTCTACTATAAAA AATGTCCTTCTGTAGTTAGACATTTGGCTGTTTTCCCTGACACGATCACTGGAGCAGATTCTTCCCAGTT GCTAGAGGTGTCAGGCTCCTGCGTCAACCATTCTGTGACAGACGATCCTCCCAAAATGCATTGCAGTGCT GAAGGGGAGTGGCTGGTTCCCATCGGGAAATGCATGTGCAAGGCCGGATATGAAGAGAAAAATGGTACCT GTCAAGTGTGCAGACCTGGGTTCTTCAAAGCCTCTCCTCACAGCCAGACCTGCAGCAAATGTCCACCTCA CAGTTACACCCATGAGGAAGCTTCCACCTCTTGTGTCTGTGAAAAGGATTATTTCAGGAGGGAATCTGAT CCGCCCACAATGGCATGCACAACTCCTTCTCCAGTCACCAACGTGAAGAAGGGGAAAATCGCAAAGAACA GCATTTCTTTGTCTTGGCAAGAGCCAGATCGCCCCAATGGGATCATCCTGGAGTACGAAATCAAGTACTT TGAAAAGGACCAAGAGACCAGCTACACAATTATCAAGTCTAAAGAGACCACTATTACGGCAGAGGGCCTG AAACCTGCGTCTGTGTATGTCTTCCAAATTCGAGCACGTACAGCAGCAGGCTACGGCGTCTTCAGTCGAC GGTTTGAGTTTGAAACCACACCAGTGTTTGCAGCATCTAATGATCAAAGCCAGATTCCCATTATTGCGGT GTCAGTGACGGTGGGAGTCATCTTGTTGGCAGTGATGATCGGCTTCCTCCTCAGTGGCAGTTGCTGCGAA TGTGGCTGTGGGAGGGCTTCTTCCCTGTGCGCTGTTGCCCATCCAAGCCTAATATGGCGGTGTGGCTACA GCAAAGCAAAGCAGGATCCAGAAGAGGAAAAGATGCACTTTCATAACGGGCACATTAAACTGCCAGGAGT AAGAACGTACATTGATCCACACACCTACGAAGATCCCACTCAAGCCGTTCATGAATTTGCCAAGGAGATC GAGGCCTCATGCATCACCATTGAGAGAGTTATTGGAGCAGGTGAATTTGGTGAAGTTTGTAGTGGACGTT TGAAACTACCCGGAAAAAGAGAATTGCCTGTGGCTATCAAAACTCTTAAAGTAGGCTACACTGAAAAGCA GCGCAGAGATTTTCTGAGTGAAGCGAGTATCATGGGGCAGTTTGATCATCCAAACATCATCCATCTAGAA GGCGTTGTGACTAAAAGTAAACCTGTGATGATAGTGACAGAGTACATGGAGAATGGCTCCTTAGACACAT TTTTAAAGAAAAACGATGGCCAGTTCACTGTGATTCAGCTTGTTGGCATGCTGAGAGGCATCGCTGCAGG AATGAAGTACCTTTCTGACATGGGCTACGTGCACAGAGACCTTGCTGCTAGAAACATCTTAATCAACAGT AACCTTGTGTGCAAAGTGTCTGACTTTGGACTTTCCAGGGTGCTGGAAGATGATCCTGAGGCAGCCTATA CCACAAGGGGAGGCAAAATTCCAATCAGGTGGACTGCTCCAGAAGCAATAGCTTTTCGAAAGTTTACCTC TGCCAGTGATGTCTGGAGCTATGGAATCGTAATGTGGGAAGTTGTGTCCTATGGAGAGAGACCGTACTGG GAGATGACCAATCAGGATGTGATCAAGGCAGTGGAAGAAGGCTACCGCCTGCCAAGCCCCATGGATTGTC CTGCTGCCCTCTATCAATTAATGCTGGATTGCTGGCAGAAAGATCGCAACAGCAGGCCCAAGTTTGATGA CATAGTCAACATGCTGGATAAGCTGATACGAAACCCAAGTAGTTTGAAGACACTGGTGAACGCGTCAAGC AGGGTATCTACTTTGTTGGCAGAACATGGATCTTTGGGGTCTGGGGCCTACAGATCAGTAGGTGAATGGC TAGAAGCAATCAAAATGGGCCGGTACACAGAGATTTTCATGGAAAATGGATACAGTTCAATGGACGCTGT GGCTCAGGTGACCTTGGAGTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_024367
Insert Size	2682 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_024367.1, NP_077343.1
RefSeq Size	3464 bp
RefSeq ORF	2682 bp
Locus ID	79208
UniProt ID	P54757
Gene Summary	receptor tyrosine kinase; may have a role in both the development and maintenance of differentiated neurons [RGD, Feb 2006] Transcript Variant: This variant (2) lacks an alternate in-frame exon in the central coding region and uses an alternate splice pattern in the 3' coding region, compared to variant 1. The resulting isoform (2) lacks an internal segment and has a shorter and distinct C-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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