Gria3 (NM_001112742) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	GluA3; GluR-3; GluR-C; GluR-K3; GLUR3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217303 representing NM_001112742 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCAAAGCGTGCTCCGGGCGGTCTTCTTTTTAGTCCTGGGGCTTTTGGGTCATTCTCACGGAGGAT TCCCCAACACCATCAGCATAGGTGGACTTTTCATGAGAAACACGGTTCAGGAGCACAGCGCTTTCCGCTT TGCTGTGCAGTTATACAACACCAACCAGAACACCACTGAGAAGCCCTTCCATTTGAATTACCACGTAGAC CACTTGGATTCCTCCAATAGTTTTTCTGTGACTAATGCTTTCTGCTCCCAGTTCTCCAGAGGGGTGTATG CTATCTTTGGATTCTATGACCAGATGTCAATGAACACCCTGACCTCCTTCTGTGGGGCCCTGCACACATC TTTTGTCACACCTAGCTTTCCCACTGATGCAGATGTGCAGTTTGTCATCCAGATGCGCCCAGCCTTGAAG GGTGCCATTCTGAGTCTTCTCGGTTACTACAAGTGGGAGAAGTTTGTGTACCTCTATGACACAGAACGAG GGTTTTCTGTCCTACAAGCAATTATGGAGGCAGCAGTGCAAAACAACTGGCAAGTGACAGCAAGGTCTGT GGGAAACATAAAGGACGTCCAGGAATTCAGACGCATCATTGAAGAAATGGACAGAAGGCAGGAAAAACGA TACTTGATTGACTGTGAAGTCGAAAGGATTAACACAATTTTGGAACAGGTTGTGATCCTGGGGAAGCATT CAAGAGGCTATCACTACATGCTTGCTAACCTGGGTTTTACTGACATTTTACTGGAAAGAGTCATGCATGG GGGAGCCAACATTACAGGTTTCCAGATTGTCAACAATGAAAACCCAATGGTTCAGCAGTTCATACAGCGC TGGGTGAGACTGGATGAAAGGGAATTCCCTGAAGCCAAGAATGCACCACTGAAGTATACATCTGCGCTGA CACATGACGCAATATTGGTCATAGCAGAAGCCTTCCGATACCTGAGGAGACAGAGAGTGGATGTCTCCCG CAGAGGCAGTGCTGGAGACTGCTTAGCAAATCCTGCTGTGCCCTGGAGTCAAGGAATTGATATTGAGAGA GCTCTGAAAATGGTGCAAGTACAAGGAATGACTGGAAACATCCAATTTGACACTTATGGACGTAGGACAA ATTATACCATTGATGTCTATGAAATGAAAGTCTCGGGTTCTCGAAAAGCTGGTTACTGGAACGAATATGA AAGGTTTGTGCCCTTCTCAGATCAACAAATCAGCAATGACAGCTCATCCTCAGAGAACCGGACCATTGTA GTGACTACCATTCTGGAATCACCATATGTGATGTATAAAAAGAATCATGAGCAGCTGGAAGGAAATGAGC GCTATGAAGGCTACTGTGTTGATTTAGCCTATGAAATAGCCAAACACGTAAGGATCAAATACAAATTGTC CATTGTCGGTGATGGGAAATATGGCGCCAGAGATCCAGAGACTAAAATATGGAATGGCATGGTTGGGGAA CTTGTCTATGGAAGAGCTGATATAGCTGTTGCTCCACTCACTATAACATTGGTCCGTGAAGAAGTCATAG ATTTCTCAAAGCCATTTATGAGCCTGGGAATCTCCATCATGATAAAGAAGCCTCAGAAATCAAAGCCAGG CGTCTTTTCATTCCTGGATCCTTTGGCTTATGAAATCTGGATGTGCATTGTCTTCGCTTACATTGGAGTC AGTGTAGTTCTCTTCCTAGTCAGCAGATTTAGCCCTTATGAATGGCACTTGGAAGACAACAATGAAGAAC CTCGTGACCCACAAAGCCCTCCTGATCCTCCCAATGAATTTGGAATATTTAACAGTCTTTGGTTTTCCTT GGGTGCTTTCATGCAGCAAGGATGTGATATTTCTCCAAGATCACTTTCTGGGCGCATTGTTGGAGGGGTT TGGTGGTTCTTCACCCTGATCATAATCTCTTCCTACACTGCAAACCTTGCTGCTTTCCTGACTGTGGAGA GGATGGTGTCCCCTATAGAGAGCGCTGAAGACTTAGCCAAGCAGACTGAAATTGCATATGGGACCCTGGA CTCTGGTTCAACAAAAGAATTTTTCAGACGATCCAAAATTGCTGTGTATGAGAAAATGTGGTCTTACATG AAATCCGCAGAGCCATCTGTGTTTACCAAAACAACAGCTGACGGGGTAGCCCGAGTTCGGAAGTCCAAGG GAAAGTTCGCCTTCCTGCTGGAGTCGACCATGAACGAGTACATTGAGCAGAGAAAGCCGTGCGATACGAT GAAAGTTGGTGGAAATCTGGATTCCAAAGGCTATGGTGTGGCAACCCCTAAAGGCTCAGCATTAGGAAAT GCTGTTAACCTGGCAGTATTAAAACTGAATGAGCAAGGCCTCTTGGACAAATTGAAAAACAAATGGTGGT ACGACAAAGGAGAGTGCGGCAGCGGGGGCGGTGACTCCAAGGACAAGACCAGTGCTCTAAGCCTGAGCAA TGTGGCAGGCGTGTTCTATATACTTGTCGGAGGTCTGGGCCTGGCCATGATGGTGGCTTTGATAGAATTC TGTTACAAATCACGGGCAGAGTCCAAACGCATGAAACTCACAAAGAACACCCAAAACTTTAAGCCTGCTC CTGCCACCAACACTCAGAATTACGCTACATACAGAGAAGGCTACAACGTGTATGGAACAGAAAGTGTTAA GATCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001112742
Insert Size	2667 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001112742.1, NP_001106213.1
RefSeq Size	5052 bp
RefSeq ORF	2667 bp
Locus ID	29628
UniProt ID	P19492
Gene Summary	Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes composed of multiple subunits, arranged to form ligand-gated ion channels. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. The subunit encoded by this gene belongs to a family of AMPA (alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate)-sensitive glutamate receptors, and is subject to RNA editing (AGA->GGA; R->G). Alternative splicing at this locus results in different isoforms, which may vary in their signal transduction properties. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) uses an alternate internal coding exon compared to transcript variant 1, and encodes an isoform (2, also known as flop isoform) that is the same length, but with a few amino acid differences from isoform 1. RNA editing (AGA->GGA) changes Arg769Gly. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.

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