Dlgap3 (NM_001301876) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	DAP-3; Dap3; SAPAP3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217298 representing NM_001301876 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTACCCGGGCCAGGGCCCCTTCGACACCTGTGAAGACTGTGTGGGCCACCCACAGGGCAAGGGTGCCA CCCGCCTGCCTCCCACACTCCTGGATCAGTTTGAAAAGCAGTTGCCAGTTCAACAAGATGGCTTCCACAC ACTCCCATACCAGCGAGGTCCAGCAGGGCCTGGTCCTGGACCTGGATCTGGCGCTGCCCCTGAGGCTCGC AGCGAGAGTCCTAGCCGCATCCGGCACCTGGTTCACTCTGTGCAGAAGCTCTTTGCCAAGTCCCACTCTC TGGAGGCACCTGGGAAGCGAGACTATAATGGGCCTAAGGCCGAGGGAAGAAGTAGCTCTGGGGGAGACAG CTACTCTGGTCCAGGCTCAGGAGGCCCCCCCACCTCCCACCACCACCATCACCACCACCACCATCACCAC CACCAGTCCCGCCATGGCAAACGGAGCAAGAGCAAGGACCGCAAGGGGGATGGGCGACACCAGACCAAGG CCACAGGCTGGTGGAGCTCTGATGACAATTTGGACAGTGACAGTGGCTTTCTGGGTGGGAGGCCCCCTGG AGAGCCCGGAGGTCCCTTCTGCCTGGATGCTCCAGATGGGTCCTACCGGGACTTGAGCTTCAAGGGGCGT TCGGGCGGGTCAGAAGGCCGCTGCCTTGCCTGCACTGGCATGTCCATGTCCCTGGATGGACAGTCAGTCA AGCGAAGTGCCTGGCATACCATGATGGTCAGCCAGGGCCGGGATGGATACCCAGGGGCCGGGCCAGGCAA GGGGCTCCTGGGTCCAGAGACCAAGGCCAAAGCCAGGACTTATCACTATTTGCAGGTGCCGCAAGATGAC TGGGGGGGTTACCCCACGGGTGGCAAGGATGGGGAGATCCCCTGCCGCAGGATGCGGAGCGGCAGCTACA TCAAAGCCATGGGGGACGAGGAGAGCGGAGACTCAGATGGTAGCCCCAAGACGTCTCCCAAAGCACTTGC CCGACGCTTTGCCTCCCGCCGTTCCTCCAGCGTGGACACAGCCAGGATCAACTGCTGTGTCCCACCCCGG ATCCATCCCCGGAGTTCCATCCCTGGCTACAGCCGTTCCCTTACAACTGGACAGCTAAGCGAAGAGTTTA ACCAGCAGCTGGAGGCCGTGTGCGGGTCCGTGTTTGGGGAGCTTGAGTCCCAGGCCGTGGACGCCCTGGA CCTGCCCGGCTGTTTCCGCATGCGGAGCCACAGCTACCTCCGGGCCATCCAGGCCGGCTGCTCTCAAGAC GACGACTGCCTACCCCTCCTTGCTGCCCCTGCCTCTGTCTCAGGGAGGCCCGGCTCCTCCTTCAACTTCA GAAAGGCCCCGCCCCCTATCCCGCCGGGAAGCCAGGCCCCACCCCGCATCTCCATCACCGCCCAGAGCAG CACCGACTCTGCCCACGAGAGCTTCACTGCAGCAGAGGGCCCGGCCCGGCGCTGCAGCTCCGCGGACGGA CTGGACGGCCCCACCATGGGCGCACGCACCCTCGAGTTGGCACCGGTGCCACCCCGGGCCAGCCCCAAGC CCCCCACACTCATCATCAAGACCATTCCTGGCCGGGAGGAGCTGCGGAGCCTGGCGCGACAGCGGAAGTG GCGGCCGTCCATTGGGGTGCAGGTGGAGACAATCTCAGACTCGGACACCGAGAACAGGAGCCGAAGAGAG TTCCACTCCATTGGCGTTCAGGTGGAAGAGGACAAGAGGCGGGCGAGGTTCAAGCGCTCCAACAGTGTGA CAGCCGGTGTGCAGGCAGACCTGGAACTGGAGGGCCTGGCTGGCCTGGCCACTGTGGCCACAGAAGACAA AGCCCTGCAGTTCGGACGCTCCTTCCAGAGGCACGCCTCTGAGCCCCAGCCCGGACCCCGGGCCCCCACC TACTCAGTCTTCCGCACGGTCCACACGCAGGGCCAGTGGGCCTACCGCGAGGGCTACCCACTGCCGTATG AGCCGCCGGCCACCGATGGGTCGCCTGGCCCTCCCCCTGTCCCCGCCCCCGGCCCCGGGTCCGGCCGCCG TGATTCCTGGATGGAGCGGGGTTCGCGTAGCCTCCCCGACTCAGGCCGCACGTCCCCCTGCCCACGGGAT GGCGAGTGGTTCATCAAGATGCTGCGGGCAGAGGTGGAGAAACTGGAACACTGGTGCCAGCAGATGGAGC GGGAGGCGGAGGACTATGAGCTGCCGGAGGAGATCTTAGAGAAGATTCGAAGCGCCGTGGGCAGCACACA ACTTCTCCTGTCTCAGAAGGTCCAGCAGTTCTTCAGGCTGTGTCAGCAAAGCTTGGACCCCACTGCGTTC CCTGTGCCCACCTTCCAGGACCTGGCCGGTTTCTGGGACCTCCTGCAGCTCTCCATTGAGGATGTGACCC TCAAGTTCCTGGAGCTACAACAACTCAAGGCCAACAGCTGGAAACTCCTGGAGCCTAAGGAGGAGAAGAA GGTCCCTCCGCCGATACCAAAGAAGCCCTCGCGGGGGCGGGGCGTGCCGGTGAAGGAGCGCTCCCTGGAC TCGGTGGACCGGCAGCGGCAGGAAGCGCGCAAGCGGCTCCTGGCGGCCAAGCGCGCCGCCTCGTTCCGCC ACAGTTCGGCTACTGAGAGCGCGGACAGCATCGAGATCTACATCCCCGAGGCCCAGACCAGGCTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001301876
Insert Size	2658 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001301876.1, NP_001288805.1
RefSeq Size	3873 bp
RefSeq ORF	2658 bp
Locus ID	286923
Gene Summary	binds neuronal cell guanylate kinase PSD-95/SAP90; may play a role in post synaptic density structure and synaptic function [RGD, Feb 2006] Transcript Variant: This variant (1) represents the longer transcript which can encode two isoforms based on alternate in-frame translation starts (PMID: 227619920). The shorter isoform (3, also known as beta) represented in this RefSeq is translated from the downstream AUG start codon. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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