Ap5b1 (NM_001271032) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Beta5; RGD1562657
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217290 representing NM_001271032 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTCCCCTGAGTCGAGAAGCCTGGGCCCAGCGCCTGGGGTCTTTCCGAGCCAGCCCATCCGCCTTTC TAGCAGGTGCTGAAGGTGAGGATTTGGGTCACGACCTGTTGAGTGACCTAAGGAGTGAAAAGCTAAGCGA GCTGATCAAGGTTTCCTTACTGACACTGAGCTTGGAGTACTCTGACAAACTTTGGCCTGATGCCCTTGCC GCGGAGGCTGCTGCCACTTCCTTGTTGGACACGCTTGTCCTCTTGCCCTCAAAACCTTCAGCTCTCCGGA GGCTCCTGTTGTTAGCAGCCACTACAGCCCTGGTGTCAGGTGGTGCTCTCGGACCCACTTCGGAAGCTTC CAGCCGGCTCCTGCCCTTACTTCTTGGCTTAGCTTCTGGCCAAGACATGGGACGAAGCTTTGGAACCACC TCAGAGCAGCGCCATCTACAGGCCACAGCGTGTGAATGCCTTGGAGAACTTGAGCGCTGTAAACCTGGGT TGCTGGCCGGCGCTCTGGGGGTGCTGCGGAGCCTCCTAGGGCAAAAGGGTCCCATCCAGCCTGTCAGCCT GCTTCTCGCCCTTGTTCTGCATAACACCTTGGTGGTACAGTCCAGGTTTGGGGCTGGCCTGCAAGGCCTG CTTGTGGCTAAGGACTCCTCCCCTGGGAGTTGTCCCTGGGACTGGACACTGGCTGAAGAATGGGATGACC ATCTTAAGCCCCAGGCGCACGGCTGGCCCACAGCTGGGGAGGAGGAACGTGACTTTCCAATTCTGGATCC CAACCCTGAGGACACTCGAGAGCTGAAGGCTGCGGTGGCCCAGCTTCTGGACACTTCCTATCTGCTCACT CCCGTGGCTCAGGCCCAACTTGTGTGGCTGTTGGGCTGGGCCCTTCAGGGTCTTCGGGGACAGCCACCAG TGCTTTTCAAGCCACAGCTAGTTCGGCTGCTGGGCACAGCACAGCTTACACTGTTACACTCAGTTCTATC TCTCAAGGCAGCCTTCGGTGAGGCCCTGTTCACTGCTCAGGATGAAGCGCTCCTGCTCCGTAGGCTTACC CTGGTGGCCCAGCACCCAGCCTTACCCTCGCCCACACACCTCTTTTACCTGCACTGCATCCTGAGCTTCC CTGAGAATTGTCCATTAGGTCCAGAAGGGGAAGAGGCTGCCCCACTGCTGTTGGGGCCCCAGTTACGCCG GGGCCTCATGCCCAGTCTCCTTCATGACCCAATGGTCCTCCTGGCCCGCCTGCATTTGCTATGCCTGCTT TGTGCTGATGATGAGGAAGAGGAGAAAGGCCAGCTTCAGGGCCCACAGTGGTTCCTGCAGGAGCTGCTGG CCGGTCTTCAGCAGAGGGCAGCCGTGGATGGCGGTCCCCGAGCCTTGGCCACTCTCTGTTTCCAGGCCTC ATACCTCGTTACCAGCTGCCTGACCAGACAGCCCACAGTCCAGACATCTCTGGTCCATGGGCTGGCCCAA CTGTACCGAGCCCGACCTTCCCTGGCTCCCCACTTTGTGGACCTCTTAGATGAGGTAAGCCCTGAGCTGA GAGAGCCCCTCAGGAAGGTGCTGCTACGAGAGGTGGTAGCCAGGCCAGGCAAGAACGAAGCACTTCGCTG GCACCTGCAGATGCTGGCAAAGGTGGCAGAGGGAGCCACTCAGAGTGCCATCCTTGGCTTTCTACAGGCT GCAGCCATACACTGCACCGACTGGGGCCTACACCAGGCCCTACTGCGGGTGTGCCGTGCTCTGCTCCGGA CGGGTGGGGGAGAGGGGCTGGCAGACCTGTTGCAGGAACTGGCCAGACAGCTGGAGAACGCTGATGGGCG GGACCATGCCCGACTGTACTATGTCCTCTTGTCCCATCTGTCAAGTTCTAAGTTGGGGATGGCACTGGGC CCCTCACTGGCTGCCCCTGCGCTGGCCTCGTCACTGATGGCTGAGAACCAGGGCTTCGCATCAGCACTGA TGGTGCAGGAGACCTCAGCTCCAATTCAGTTGAGTGTGGGGCCTCAGAAGGCCAAAGGCCCACTCCCGGT GCTGCATCTGCAGGTGCAGGCACTGGATACTCCTGTGTACTCTCTGGAGCTGCGCTTTCGTGTAGAAGGA CAACTGTACGAGCCTGTGGAGGCTGTCCACATACCCTCTGTGCGTCCAGGACAACCTGCCCATCCTCTGC ACCTGCCTTTGCAGCCCCGTAGCCCAGCCCCCGCCCGCCTGCATGTCCGGGCCCTTTACACCACACCGGC CGGCCTCACGTGCCATGCCCACCTACCACCCCTGTCTGTGAACTTTGCTGACCTCTTCCTGCCTTTCCCC CAGCTCCCCAAGGGCTCTGAGTTGTGTTTCTTTGATGAGCTCTGGAACTCCTGCCTGCCAAAGGGTGTAG AAAGTCGTGTGTGGTGTCCACTTGGGCCACAGGGCCTGGAGGCCTTGGTGTCCCAACACCTAGAGCCCTT TGTCGTGGTGGCGCAGCCCCCTACCACCTACCTCATAGCTGTCCGCTTGCCTCCTCACTCCATGCTGCTG CTGCGCCTGGAGAAAGCTCAGGTGCACGGTGTGCCTGTGGCCCTCAGGACTGATGACTGGGCTGTGCTGC CCCTGGTGGGGGACTACCTCCGAGGGCTTGCAGCCCACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001271032
Insert Size	2631 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001271032.1, NP_001257961.1
RefSeq Size	3551 bp
RefSeq ORF	2631 bp
Locus ID	361709
UniProt ID	D3ZVB0
Gene Summary	As part of AP-5, a probable fifth adaptor protein complex, it may be involved in endosomal transport.[UniProtKB/Swiss-Prot Function]

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