Il12rb2 (NM_001191750) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217281 representing NM_001191750 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCACACACTGTTAGAGGATGCTCATTGGCACTTATTTTTTTGTTCATGTGGCTGTTGATTAAAGCAA ATATAGATGTGTGCAAGCTTGGCACCGTGACTGTCCAGCCTGCCCCTGTGATTCCACTTGGGTCAACTGC CAATATTTCCTGCTCTTTGAATCCCAAACAAGGCTGTTCGCATTATCCTAGTTATAACGAATTAATCCTC TTAAAGTTTGTCAATGATGTCCTTGTCGAAAAACTCCATGGCAAAAAAGTCCATGGCCACCCTAGTCATT CCTCCATATTTCAAGTCACCAACCTGTCTCTTGGTATGACTCTGTTTGTCTGCAAGCTAAACTGTAGCAA CTCTCAAAAGACACAACCAGTGCCAGTGTGTGGAGTGGAGATCTCAGTCGGTGTTGCTCCAGAGTCACCT CAAAGCATATCATGTGTCCAGAAAGGAGAGCATGGAACTGTGGCCTGCTCCTGGAACTCTGGGAAAGTTA CTTATCTGAAAACCTATTACACTTTACAGTTAACTGGACCAAACAATGTGACCTATCAGAACCAGTGTTA TTCTGACAATCGTCAGAATTGTAGTCGTCTGGATCTTGGGATCAATCTGACCCCTGATTTAGCCGAATCC AGGTTCATAGTCCATGTTACTGCCACCAATGATCTTGGAAATTCTTCTTCACTTCTGCATACATTCACAT TCTTGGACATAGTGAGGCCTCTTCCTCCGTGGGACATCAGAATCAACTTTCTAAATGCTTCTGGGAGCAG ATGTACACTGCAGTGGGAAGATGAGGGGCAAGTGGTACTCAATCGACTCAGATACCAGCCTCTTAACAGC ACATCCTGGAATATGGTTAATGCTACAAATGCCAAAGGAAGATATGACCTGCAAGATCTGAGACCATTCA CAGAATATGAATTTCAGATCTCCTCTAAGCTACATCTCTCTCAGGGAAGTTGGAGTAATTGGAGTGAGTC ACTGAGAACTCGAACACCAGAGGAAGAGCCTGTTGGGACATTAGACATCTGGTACATGAAACAAGATATT GACTGTGACAGACAACAGATCTCTCTTTTCTGGAAGAGTCTGAATCCATCAGAGGCAAGGGGGAAGATCC TCCACTATCAGGTGACTTTACAGGAGGTGACGAAGAAAACTACACTGCAGAATACTACAAGACATACCTT CTGGACCGGGGTCATTCCCAGAACTGGGGCTTGGACTGCATCTGTGTCTGCAGCCAACTCAAAAGGCACT TCTGCACCCACTCACATTAACATAGTGGACCTGTGTGGCACTGGGTTCCTGGCTCCTCACCAGGTCTCTG CAAAGTCAGGGAACATGGACAACATCATGGTGACCTGGCAGCCTCCTGGGAAAGCTGACTCTGTGATTCG GGAGTACATAGTGGAATGGAGGGCTCTCCAACAAGGGAGCGTCATGAACCTTACCCCACACTGGCTGCGA ATTCCCCCCTACAACATGTCTGCTCTGATTTCAGAGAAGATAAAGCCCTATATCTGTTACGAAATCCGGG TACATGCACTGTCAGAGGACCAAGGAGGGTGCAGCTCCATCCGGGGTGACTCCAGTCACAAAGTGCCACT GAGTGGCCCTCATATCACTGCTATCACAGAGAAAAAGGAAAGCCTTTTTATTTCCTGGACCCACATCCCT TTCCTGGAGCAAAGGGGCTGCATCCTCCATTACAGAATATATTGGAAGGAGCGACACTCTACTGCGCAAC CCGAGCTCTGTGAAATTCAGTACAGACACTCTCAAAACTCACATCCAATAAGCAGCCTACAGCCCAAGGT GACATATGTCCTATGGATGACAGCTGTGACAGCTGCTGGTGAAAGTCCCCAAGGAAATGAAAGGGAATTT TGTCCACAGGGCAAAGCCAACTGGAAAACATTCGTGATATCAAGCATTTGCATCGCTATCATCGTGGTGG GCACTTTCTCAATTCGTTACTTCAGGCAAAAGGCATTTACTCTCCTTTCTACTCTCAAACCCCAATGGTA TTGCAGAACTATTCCAGATCCAGCAAATAGCACTTGGGTAAAGAAGTACCCCATTATGGAGGAGAAGATC CAGCCACCTATGGACAATCTCCTGATGGCCTGGTCCGCTCCTGAAGAGCCTGAGCCCCTGATCATCAATG AAGTCCTCTACCAAATGATCCCAGTAGGCAGACAACCCTATTGCTTCAAAAGAGGACAAGGGTTCCAAGG TTACTCTACCTCCAAGGAAGACACAATATACCTTGCCACCAATCCACAAACTACAGGAACTCTCACAGCT GAGTCGAGACAACTAGTGAATCTATACAAGGTGCTAGGAAGCAGGGACCCTGACTCAAAACTGGGCAACC TGACCAGCCCCTTGACAGTCACCCCAGTGAACTACCTTCCTACCCATGAAGGCTATTTACCCTCCAACAT AGAAGACCTCTCATCACATGAAGTTGACCCAAGTGATTCTTTAGACCTGGACCATCAACATATTTCTCTT TCCATTTTTGCATCAAGTTCTCTCCGCCCACTCACCTTCTGTGGTGAGAGGCTGACTCTAGATCGGTTAA AGATGGGCTACGAGAAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001191750
Insert Size	2610 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001191750.1, NP_001178679.1
RefSeq Size	2610 bp
RefSeq ORF	2610 bp
Locus ID	171334
Gene Summary	transmembrane protein that belongs to the hemopoietin receptor superfamily; human homolg binds interleukin (IL)12 with a low affinity and may be a part of the IL12 receptor complex [RGD, Feb 2006]

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