Trpc3 (NM_021771) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	TrpC3c; Trrp3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217251 representing NM_021771 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGAGGCCGAGGGAGCCGAGCCGCAGCGCCGCCGCCGGGGCTGGAGGGGCGTCAACGGCGGGCTGGA GCCGCCCTGCCCGCGCGCGCCGCCGTCCCCGGGGCCCGACGCAGTACGGCAACATCCCTGTGGTGCGCAA GATGCTGGAGGAGTCTCGCACGCTCAATGTCAACTGCGTGGACTACATGGGCCAGAATGCGCTGCAGCTG GCCGTGGGCAATGAGCATCTGGAGGTGACCGAGCTGCTGCTGAAGAAGGAGAACCTGGCGCGTATCGGTG ATGCGCTGCTGCTCGCCATCAGCAAAGGCTATGTGCGCATCGTGGAGGCCATCCTAGGCCACCCAGGCTT TGCGGCCAGCCGGCGCCTGACCCTCAGCCCTTGCGAGCAAGAACTGCGCGATGACGACTTCTATGCTTAC GACGAGGATGGCACACGCTTCTCACCTGACATCACGCCCATCATCCTGGCTGCGCATTGCCATAAATACG AGGTGGTGCACCTGCTGCTACTCAAGGGTGCACGCATCGAGCGGCCACACGACTACTTCTGTCGCTGCGC CGACTGTGCTGAGAAGCAAAGGCTTGATGCCTTCAGCCACTCAAGGTCTAGGATCAATGCCTACAAGGGA CTGGCCAGCCCAGCATACTTGTCGCTGTCCAGCGAGGACCCTGTGCTCACAGCGCTGGAACTCAGCAATG AGCTGGCCAAGCTGGCCAACATAGAGAAGGAGTTCAAGAATGACTACAGGAAGCTCTCCATGCAGTGCAA AGACTTCGTAGTAGGTGTGCTGGACCTGTGCCGGGACTCAGAGGAGGTGGAAGCCATTCTGAATGGAGAT CTGGAATCGGTGGAACCCCTGGAGAGACACGGGCACAAGGCGTCGCTGAGTCGGGTCAAACTTGCCATTA AATATGAAGTCAAAAAGTTTGTGGCTCACCCCAACTGCCAACAGCAGCTTTTGACCATCTGGTACGAGAA CCTCTCGGGCCTTCGGGAGCAGACCATCGCTATCAAGTGTCTGGTCGTGTTGGTCGTGGCCTTGGGCCTT CCATTCCTCGCCATCGGCTACTGGATTGCACCTTGTAGCAGGCTGGGGAAAATTCTTCGAAGCCCCTTCA TGAAGTTCGTGGCTCACGCTGCCTCCTTCATCATCTTCCTGGGTCTGCTTGTGTTCAACGCCTCAGACCG GTTTGAAGGCATCACCACGCTGCCCAACATCACCGTTATTGACTACCCCAAGCAAATCTTCAGGGTGAAG ACCACCCAGTTCACATGGACAGAAATGCTAATTATGGTCTGGGTTCTCGGGATGATGTGGTCTGAGTGCA AGGAGCTGTGGCTGGAGGGGCCCCGGGAGTACATCGTGCAGCTGTGGAACGTGCTTGACTTCGGGATGCT CTCCATCTTCATTGCTGCCTTCACCGCCAGGTTCCTAGCGTTTCTGCAAGCCACCAAAGCGCAGCAGTAT GTGGACAGCCACGTGCAGGAGAGCGACCTGAGCGAAGTCACACTCCCACCAGAGGTTCAGTATTTCACCT ATGCTAGAGATAAATGGCTTCCTTCTGACCCTCAGATCATATCGGAAGGCCTCTATGCCATAGCTGTGGT GCTCAGCTTCTCCCGGATCGCGTACATTCTCCCTGCAAACGAGAGCTTTGGGCCCTTGCAGATCTCTCTG GGGAGGACTGTGAAGGACATATTCAAGTTCATGGTTCTCTTCATCATGGTGTTCCTGGCTTTCATGATTG GCATGTTCATACTTTACTCCTACTACCTTGGGGCCAAAGTAAACCCTGCTTTTACCACGGTTGAAGAAAG TTTCAAGACTTTGTTTTGGTCCATATTTGGACTCTCTGAAGTGACTTCTGTTGTCCTCAAATATGACCAC AAATTCATAGAGAACATTGGCTATGTCCTTTATGGAATATACAATGTAACTATGGTGGTCGTTCTGCTCA ACATGCTGATTGCTATGATTAACAGCTCATACCAAGAAATCGAGGATGACAGTGATGTAGAGTGGAAGTT TGCTCGTTCCAAACTCTGGCTATCCTACTTCGATGATGGAAAAACATTACCTCCACCCTTCAGTCTGGTC CCTAGTCCAAAATCGTTTGTTTATTTCATCATGAGGATCACTAACTTTTCCAAATGCAGGAGGAGAAGAC TTCAGAAGGATCTGGAACTGGGCATGGGTAACTCAAAGTCCAGGTTAAACCTCTTCACTCAGTCTAACTC GAGAGTTTTTGAATCACACAGTTTTAACAGCATTCTCAATCAGCCAACACGATATCAGCAGATAATGAAA AGACTCATAAAACGGTATGTTTTGAAAGCACAAGTAGACAAAGAAAATGATGAGGTGAACGAAGGTGAAC TGAAAGAAATCAAGCAGGATATCTCCAGCCTTCGTTACGAACTTTTGGAAGATAAGAGCCAAGCGACGGA GGAACTGGCCATCTTGATTCATAAACTCAGTGAGAAACTGAACCCCAGTGCGCTGAGGTGTGAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_021771
Insert Size	2517 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_021771.2, NP_068539.2
RefSeq Size	3420 bp
RefSeq ORF	2517 bp
Locus ID	60395
Gene Summary	a calcium-activated cation channel [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.