Gpat2 (NM_001168529) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	RGD1304904
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217218 representing NM_001168529 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGACCATGTTGAAGTCCAACCCTCAAATGCAGCAGAGGAATAACCACAGTGGTCAAGAGACCAGCC TTTGGTCCTCAGGCTTTGGGATGAAGATGGAGGCCATCACTCCATTCCTGGGGAAGTATCGCCCCTTTAT GGGTCGATGCTGCCAGACCTGTACCCCTAAGAGCTGGGAGTCCCTCTTCCACAGAAGCATAATGGATCTG GGCTTCTGCAATGTGATCCTTGTGAAGGAGGAGAATACCAGGTTTCGGGGCTGGCTGGTTCGCAGGCTCT GCTATTTCCTATGGTCCCTGGAGCAGCACATACCCACCAGCTCTGATGCCTCCCAGATGATCATGGAAAA CACTGGGGTGCAGAACATCCTCTTGGGGAAGGTTCCAGGAGCGGCTGGAGAAGGCCAGGCACCTGACCTA GTGAAGAAAGAGGTACAGCGCATCCTGGGCCACATTCAGACCACACCCCGTCCCTTCTTGCTTAGGCTGT TCAGCTGGGCACTGCTGTGGTTCCTGAACCGCCTCTTCCTGAACGTTCAACTTCACAAGGGACAGATGAA GATGGTCCACAAGGCTGCCCAGGAGGGCTCTCCACTTGTCTTCCTTTCTACACACAAGTCCCTCTTGGAT GGGTTCCTGCTGCCTTTTGTACTGTTCTCCCAAGGGCTCGGTGTGCTCCGTGTGGCCTTGGACTCCCGTA CTTGTTCCCCTGCTCTCAGAGCTCTACTGAGGAAACTTGGGGGGCTTTTCCTGCCCCCAGAGGCCAACCT CTCATTGGACAGCTCAGAGGGCATCCTTGCAAGGGCTGTGGTCCGTGCAACGGTGGAGCAGCTGCTTACT AGTGGTCAGCCCCTGCTCATCTTCCTGGAAGAGGCCCCTGGGTACCCAGGGCCCAGGCTTTCTGCCCTGG GCCAGGCCTGGCTAGGACTGGTAGTCCAGGCGGTCCAGGCAGGCATCGTCCCAGATGCCACACTGGTACC AGTTGCCACTGCCTATGATCTGGTTCCAGATGCACCATGTAACATGACTCATGACCTGGCCCCCCTGGGG CTATGGACAGGAGCCTTGGCCATCTTTCGGCGACTGTGCAACTGCTGGGGCTGTAACCGTAGAGTCTGTG TCCGGGTGCACCTTGCCCAGCCATTTTCCCTCCAGGAATATACCATCAATGCTCGAAGTTGCTGGGGTAG CAGGCAGACCCTGGAACACTTGTTGCAGCCCATTGTGCTAGGTGAATGTAGTGTTGTTCCAGACACTGAG AAAGAGCAGGAGTGGACCCCACCAACTAGCCTCCTCCTGGCCCTCAAAGAAGAGGACCAGCTCCTGGTCA GAAGACTGAGCCGGCATGTTCTGAGTGCCAGTGTGGCCAGCTCAGCAGTGATGAGCACGGCCATCATGGC AACACTGTTGCTGCTGAAGCACCAAAAGGGTGTGGTCCTGTCACAGCTCCTGGGGGAATTCTCCTGGCTG ACAGAGGAGACACTGCTCCGAGGTTTTGATGTGGGCTTCTCAGGGCAGCTGCGGTGCCTGGCCCAACATA CGCTGAGCCTCTTGAGGGCACATGTGGTTCTGCTGCGTGTCCACCAAGGGGACTTGGTGGTGGTGCCACG GCCTGGCCCAGGCCTCACACATCTGGCACGCTTGAGTATGGAATTGCTGCCCACCTTCCTGAGTGAGGCT GTGGGTGCCTGTGCGGTGAGGGGGTTGCTGGCGGGCAGAGTACCACCTGAGGGGCCCTGGGAGCTGCAGG GCATAGAGCTGCTGAGTCAGAATGAACTGTACCGCCAGATCCTACTGCTGCTGCACCTGCTACCTCAAGA CCTGTTGCTGCCCCAGCCCTGCCAGTCTTCCTACTGCTACTGTCAGGAGGTGCTGGACCGGCTCATCCAG TGCGGGCTCCTGGTTGCAGAGGAGACCCCAGGCTCCCGGCCAGCCTGTGACACAGGAAGACAGCATCTGA GTGCAAAGCTGCTGTGGAAACCAAGTGGAGACTTTACTGACAGTGAGAGTGATGACTTCGAGGAGCCAGG GGGCCGCTGCTTCAGGCTTAGCCAGCAGTCTCGCTGCCCTGACTTCTTCCTCTTCCTCTGCCGCCTGCTC AGTCCCATACTCAAGGCCTTTGCACAGGCAGCCACCTTCCTCCACTTGGGGCAGCTGCCAGATTCAGAGG TGGGCTACTCAGAGAAGTTGTTGCAGTTTTTACAGGCCTGTGCCCAGGAAGAAGGGATCTTTGAGTGTGC AGACCCAAATCTCGCTATCAGTGCAATCTGGACATTCAAAGACCTGGGGGTACTGCAGCAGATACCCAGC CCTACAGGACCCCAGCTCCACCTGTCACCTACATTTGCCAGCCGGGACAATCAGGACAAGCTGGAACAAT TCATCCGACAGTTCATCTGCAGTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001168529
Insert Size	2406 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001168529.1, NP_001162001.1
RefSeq Size	2617 bp
RefSeq ORF	2406 bp
Locus ID	296130
UniProt ID	D3ZI76
Gene Summary	Transfers an acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate producing a lysophosphatidic acid (LPA), an essential step for the triacylglycerol (TAG) and glycerophospholipids. In vitro also transfers an acyl-group from acyl-ACP to the LPA producing a phosphatidic acid (PA). Prefers arachidonoyl-CoA as the acyl donor. Required for primary processing step during piRNA biosynthesis. Molecular mechanisms by which it promotes piRNA biosynthesis are unclear and do not involve its acyltransferase activity.[UniProtKB/Swiss-Prot Function]

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