Sema3g (NM_001100882) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	CSG53; RGD1306079
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217203 representing NM_001100882 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCGCTGCTCCGCTTGGGCCATCTGCTGCCTCCTGGGGAGCCTTCTGTTCCACATGGGCAGCCCGA ACCCTGGTCCCAGCACCAGTGTGCCTCGTCTGCGGCTCTCCTACAGAGATCTCCTGACTACCAACCGCTC TGCCATCTTTCTGGGTCCACGGGGCTCCCTAGACCTCCAAGTCATGTACCTGGATGAGTATCGGGACCGC CTCTTTCTGGGAGGCCGAGATGCCCTTTACTCTCTGAGACTGGATCAGGCATGGCCAGATCCCCGGGAGG TCTTGTGGCCGCCGCAGCCCGGACAGAAGGTGGAATGTGTCCGAAAGGGAAAAGACCCTTTGACCGAGTG TGCCAACTTCGTGCGGGTCTTACAACCCCACAACCGGACCCATCTGCTGGCCTGCGGCACTGGCGCCTTC CAGCCCATCTGCACCTTCATCACAGTGGGGCATCGGGGGGAGCATGTGCTCCACTTGGATCCTAGCAGCA TCGAAAATGGAAGGGGGCGTTGCCCACACGAGCCCAGCCGTCCCTTCGCCAGTACCTTTGTAGGTGGGGA GCTGTACACGGGCCTCACTGCTGATTTCCTCGGACGTGAGGCCATGATTTTCCGGAGTGGGGGTCCCCGA CCAGCCCTGCGTTCTGACTCTGACCAGAGCCTCCTACATGATCCCCGGTTTGTGATGGCTGCTCGGATCC CAGATAACTCCGACCGGGATGATGACAAAGTGTACTTCTTCTTCTCTGAGACTGTCCCTTCACCAGATGG TGGCCCAGGTCATGTCACCGTCAGCCGCGTGGGTCGAGTCTGTGTGAATGATGCTGGTGGCCAGCGGGTG CTGGTGAATAAATGGAGCACGTTCCTCAAGGCCAGGCTGGTATGCTCTGTGCCTGGCCCTGGCGGGGCTG AGACTCACTTTGACCAGCTGGAGGATGTGTTCCTGCTGTGGCCAAAGGCAGGGAAGAACCTCGAGGTGTA TGCGCTGTTCAGCACTGTCAGTGCTGTGTTCCGGGGCTTTGCTGTCTGTGTGTACCACATGGTAGACATC TGGGAGGTCTTCAACGGGCCCTTCGCTCACCGAGATGGCCCTCAACATCAGTGGGGACCCTATGGGGGCA AGGTGCCCTTCCCCCGGCCTGGTGTGTGTCCCAGCAAGATGACCGCACAGCCCGGCCGACCCTTTGGCAG CACCAAGGACTACCCGGATGAGGTGCTGCAGTTTGTCCGAGGCCACCCGCTCATGTTCCAGCCTGTGAGG CCTCGGCGTGGCCGCCCCGTCCTGGTCAAAACTCACCTGGCTCAGCAACTGTGCCAGATCGTGGTGGACA GAGTGGAGGCTGAGGATGGGACCTACGATGTCATCTTCCTAGGGACTGATTCAGGTTCTGTGCTCAAAAT CATCGCTCTCCAGGGTAGTGGCTTACCCGAACCGGAAGAGGTGGTTCTGGAGGAGCTCCAGGTGTTTAAG GTACCAACACCCATCACTGAGATGGAGATCTCTGTCAAAAGGCAAACGCTGTATGTGGGCTCTCCACTGG GCGTGGCCCGGCTGCAGCTGCACCAGTGTGAGACTTATGGCAGTGCCTGCGCTGAGTGCTGCCTGGCCCG GGACCCATACTGTGCTTGGGACGGCACTTCCTGTGCCCGCTACCGCCCCAGCTCGGGCAAGCGCAGGTTC CGACGACAGGACATCCGACATGGCAATCCTGCTGTGCAGTGTCTGGGCCAGGGCCAGAGCCAAGACAAAG CCTCGGGACTGGTGACCAGAGTCTTTGGCACAGAGCACAACAGTACCTTCCTTGAGTGCCGGCCCAAGTC TCCCCAGGCTGCAGTGCGCTGGTTCTTACAAAGGCCAGGGGATAAGGGGGCTGACCAGGTGAAGACAGAT GAGCGAGTTGTGCAGACGGACCAGGGGCTTCTGTTCCGAAGGCTCAGCCGCCTTGATGCAGGGAACTACA CCTGTACCACTCTGGAGCACGGCTTCTCCCAGACCGTGGTTCGTTTTGCCCTGGAGGTGATTGCGGCTGT GCAGCTGGACAGCCTGTTCCTTCGGGAGCCAAGGCTGGAGGAGCCCTCAGCCTGGCGAAGTGTGGCTTCT GCCTCACCCAAGACTTGGTATAAGGACATCCTTCAGCTCACTGGTTTCGCCAACCTGCCCCGCGTGGATG AGTACTGCGAACGTGTGTGGTGCAGGGGTGTCGGGGAGCGCTCGGGCTCCTTCCGCGGAAAGGGAAAGCA GGCGAAGGGCAAGAGCTGGGCGGGGCTAGAGCTGGGCAGGAAGATGAAGAGCCGGGTGCAGGCTGAGCAC AATCGGACCCCCCGGGAGGTAGAAGCCACATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001100882
Insert Size	2343 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001100882.1, NP_001094352.1
RefSeq Size	3626 bp
RefSeq ORF	2343 bp
Locus ID	290562

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