Lima1 (NM_001191615) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	eplin
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217174 representing NM_001191615 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAATCGACTCCGTTTAATAGACGGCAGTGGACTTCCCTGTCACTGAGAGTAACAGCCAAAGAACTTT CTCTCGTCAACAAGAACAAGTCGTCCACAATTGTGGAAATATTCTCCAAGTACCAGAAAGCAGCTGAAGA AGCAAACATGGAAAGGAAGAAAAATAACACTGAAAGTCTCCCGCAGCACTTTAGAAGAGGGACCCTGTCT GTGTTAAAGAAGAAGTGGGAGAACCCCGTGGCGGGAGCGGAATCCCACACAGACTCACTGCCAAACAGCA GCAGCGACGGTGGGCACACGGCGGACCACCCTCCTGCTGAAGTGACAGCCAAGGCTGCTCCTGGAGCCAG AGCTGACCGGGAAGAGCACACCCAGCCCAGATCTAGATTTGGATCTCGTCCGGAAGCTGTTACTCAGTGT CGGTATCCCCGCTCAGAGGACAGCCACGATTTTAAAGCCCAGGCCACGGAAAGCCAAAATATGGAAAACT GTCTGGGAGATTCCAGGCATGAAGCAGAGAAGCCCGAAATGAGCGAAAACACGGAAACTTCAGGCAAAAT AGAGAAATACAACGTTCCACTGAACAGACTAAAGATGATGTTTGAGAAAGGTGAACACAGCCAAAACAAG AGTCCCTGGACCCAGGGCCGAAATGCAGGCGGAAGGAGGCTCTCTGAAAACAGCTGTTCCCTGGACGATT TGGAAATAGGTGCAGGTCATTTGTCTTCCTCTGCCTTCAACTCGGAGAAAAATGAGAGTAAGCGGAATCT GGAGCTGCCACGCCTCTCTGAGACCTCCATAAAGGACCGCATGGCCAAGTACCAGGCTGCCGTGTCCAAG CAGAGCAGCCCAGCCAGCTACGCAAGTGAGCTGAAACCCAGTGAAAGCAAAACTCACAAATGGGAGCAGA AGGAGAACGTGCCCCCCGGCCCCGAGGCCTGCAGCATCCATCAGGAAGGGAGCAAGGTTTCTGCAACTGA GAATAGCCTGGTGGCCCACCCTGTCCCTGCAGAAGATGACACCTGTAACTCCCAGGGGAGGAGTGAGGCC CAGCAGCCCATATACACTAAGCCGCTGAGTCCCGACGCCAGGACCTCCAGTCTTCCGGAAAGTTCTCCTT CCAAAACAGCGAAGAAGTTTCAGGCACCCGCAAGAGAGAGCTGCGTGGAGTGTCAGAAGACGGTGTATCC CATGGAACGGCTCCTGGCCAACCAGCAGGTGTTTCACATCAGCTGTTTCCGATGCTCCTACTGCAACAAC AAACTCAGTCTAGGAACATATGCATCCTTACACGGAAGAATCTATTGTAAGCCTCACTTCAACCAACTCT TCAAGTCCAAAGGCAACTATGACGAAGGCTTTGGGCACAAGCAGCATAAGGATCTGTGGGCAAGCAAAGG TGAAAATGAAGAGACTTTGGGGAGACCAGCCCAGCCTCCCAGTGCAGGGGAGACCCCCCATAGCCCGGGT GTAGAAGATGCCCCCATCGCCAAGGTCGGCGTGCTGGCTGCGAGTATGGAAGCCAAGGCCTCCTCTCAGA GGGAGAGGGAAGAGAATAAGCCAGCGGAGACCAAGAAGCTGAGGATCGCCTGGCCTCCCCCAGCCGAGCA GGGCAGTTCAGGAAGTGCCCCGGAGGAAGGGTTCAAAGTGTCAAAGCCCAAGTGGCCTCCCGAGGATGAA GTCTGCAAGACAGAGGCGCCGGAGGATGTAGATCTGGACCTGAAGAAGCTGAGGCGCTCTTCGTCCCTGA AGGAGAGGAGCCGCCCGTTTACTGTCGCGGCTTCCTTTCGAACCTCCTCGGTCAAGAGCCCCAAGCCCTT GTCGCCGTCCCTCAGGAAGGGTTGGAGCGAGCCGGAGCCCGAGCAGAGTGAGGAGTTCGGAGGAGGGACA GTGACACAAACGGAAAGCCCCAGGCCCTCTCGGGAGAAGGAGAGTGTGGGAAAATCACGCTGGCAGAGCG AGGAAGCGGAAGCGGAAGCGGAGGAAGCGCCGAGGGGCAGGGACGGTCGTTCTTTTGAGCTGGAGAGTGA GAGTTTTATAGGAAATGGCGCAAGCATAGCTGAAGACGACGTCGCCCCAGCACAGCGGTCTCCATTAGAA CCCGAGTCTCCGGGTTGGCCTGGCTTTGGAGACACCACGACTGCTAAAGAATTCAATCAAAAATCCCAAG ATGTCGGGTTCTGGGAGGGGGAAGTGGTCCGAGAGCTGTCCGTGGAGGAGCAGATAAAGAGAAACCGGTA CTATGACGAGGATGAGGATGAGGAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001191615
Insert Size	2268 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001191615.2, NP_001178544.1
RefSeq Size	4204 bp
RefSeq ORF	2268 bp
Locus ID	300228
UniProt ID	F1LR10
Gene Summary	Actin-binding protein involved in actin cytoskeleton regulation and dynamics. Increases the number and size of actin stress fibers and inhibits membrane ruffling. Inhibits actin filament depolymerization. Bundles actin filaments, delays filament nucleation and reduces formation of branched filaments (By similarity). Plays a role in cholesterol homeostasis. Influences plasma cholesterol levels through regulation of intestinal cholesterol absorption. May act as a scaffold protein by regulating NPC1L1 transportation, an essential protein for cholesterol absorption, to the plasma membrane by recruiting MYO5B to NPC1L1, and thus facilitates cholesterol uptake (By similarity).[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.