Arhgap17 (NM_001270694) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Rich1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217169 representing NM_001270694 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGAAGCAATTCAACCGCATGAAGCAGCTGGCCAACCAGACTGTCGGCAGAGCTGAGAAGACAGAAG TCCTCAGTGAAGATCTGCTACAGATTGAACGTCGACTGGACACTGTGCGGTCAATGTGCCACCATTCACA TAAGCGTTTGATAGCCTGCTTCCAAGGTCAGCATGGCACCGATGCTGAGAGGAGACATAAAAAGCTTCCT CTGACAGCTCTCGCCCAGAACATGCAGGAAGCTTCAGCCCAGCTGGAAGAGTCTCTCTTGGGGAAGATGC TGGAAACCTGTGGGGATGCTGAGAATCAGCTGGCTTTTGAACTCTCTCAGCATGAGGTCTTTGTGGAGAA GGAGATCATGGACCCTCTCTACGGCATAGCGGAGGTGGAGATTCCCAACATCCAAAAGCAAAGGAAACAG CTTGCTAGATTGGTGCTGGACTGGGATTCAGTCAGAGCAAGATGGAACCAAGCACACAAGTCTTCAGGAA CCAACTTTCAGGGACTTCCATCTAAAATAGATACCCTAAAGGAAGAGATGGATGAAGCTGGAAATAAAGT CGAGCAGTGCAAGGATCAACTTGCAGCAGACATGTACAACTTCATGGCCAAAGAAGGGGAATACGGCAAG TTCTTTGTGACGTTATTAGAAGCCCAAGCAGATTACCATAGAAAAGCATTAGCAGTCTTAGAAAAGGCCC TTCCCGAAATGCGCGCCCATCAAGATAAATGGGCAGAGAAGCCAGCCTTCGGTACACCTCTGGAGGAACA CCTGAAGAGGAGTGGACGTGAGATCGCTCTCCCTATTGAGGCCTGCGTCATGTTGTTACTGGAGACTGGC ATGAAGGAGGAGGGCCTTTTTCGGATCGGAGCTGGAGCCTCCAAGTTGAAGAAGCTGAAAGCTGCTCTCG ACTGCTCCACGTCTCACCTGGATGAGTTCTATTCAGACCCCCATGCTGTCGCAGGTGCTTTAAAGTCCTA TTTGCGGGAACTGCCTGAGCCCTTAATGACTTTCAGTCTGTATGAAGAGTGGACACAAGTTGCAAGTGTG CAGGATCAAGACAAAAAACTTCAATATTTATGGACAACATGTCAGAAGTTGCCGCCACAAAATTTTGTTA ACTTTAGGTATTTAATCAAGTTCCTTGCAAAGCTCGCCCAGACCAGTGATGTTAATAAAATGACTCCTAG CAACATAGCCATCGTGTTGGGCCCTAACCTCCTGTGGGCCAAACAAGAAGGAACCCTGGCTGAAATAGCG GCTGCCACATCTGTCCACGTGGTCGCAGTGATTGAACCTATCATCCAGCATGCCGATTGGTTCTTCCCCG GAGAGGTAGAATTCAATGTATCAGAAGCATTCGTGCCTCTTGCTACCCCAAATTCTAATCACTCATCCCA TACTGGAAATGACTCTGACTCGGGGACTCTGGAGAGGAAGCGACCCGCCAGCATGGCAGTGATGGAAGGG GACTTGGTGAAGAAGGAGAGTCCTCCAAAACCCAAAGACTCTGTGTCTGCAGCTGCCCCTGTGGCAGGAA GAAACAGCAACCAGATAACCACGGTCCCAAACCAGGCCCAGACAGGTGGCAACTCCCATCAGCTCTCAGT AGGCACAGCTCACAGTGCAGCTGGCCCCAGCCCGCACACTCTGCGTCGAGCTGTGAAGAAACCTGCTCCG GCACCCCCCAAACCAGGAAACCCACCTCCTGGCCACCCTGGAGGACAGAGCTCTCCAGGCACAGGCACAT CCCCAAAGCCAAGCACCCGAAGCCCATCACCGCCTCAGCAGCAGCAGCAGCAGCAACAACAACAACAGCA GCAGCAACAACAACAGCAGCAACAACAACAACAACAACAGCAGCAGCAGCAGACCCCGGGCATGCGCCGC TGTTCCAGCAGCCTGCCTCCTATCCAGGCACCCAACCACCCACCACCGCAGCCCCCCACACAGCCTCGGC TGGGTGAACAGGGACCAGAGCCAGGCCCCACGCCACCTCAGACCCCCACACCACCCAGCACCCCACCCCC GGCCAAGCAGAATTCATCCCAAAGCGAGACCACACAGCTGCATGGAACCCTCCCAAGGCCACGGCCAGTG CCTAAACCCCGCAACCGGCCTAGTGTGCCACCACCCCCGAATCCACCTGGCACCCACATGGGGGATGGTG GCCTCACACCTTCAGTGCCCACAGCCTCCAGAATCGTCACTGATGCCCTTCCCGGTGCCCTCACAGGTGG GGAAGGTTTCCAGAACTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001270694
Insert Size	2259 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001270694.1, NP_001257623.1
RefSeq Size	3192 bp
RefSeq ORF	2259 bp
Locus ID	63994
UniProt ID	Q99N37
Gene Summary	activates RhoA, Rac1, and Cdc42 GTPases, plays a role in regulation of calcium ion dependent exocytosis, may regulate actin filament reorganization [RGD, Feb 2006] Transcript Variant: This variant (4) lacks an alternate in-frame exon and contains an alternate 3' exon with an in-frame stop codon compared to variant 1. The resulting isoform (4) lacks an alternate internal segment and has a shorter and distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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