Kcnd3 (NM_001270963) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Kv4.3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217027 representing NM_001270963 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCAGGAGTTGCAGCCTGGCTGCCTTTTGCCCGGGCTGCGGCCATTGGGTGGATGCCAGTGGCCA ACTGCCCCATGCCCCTAGCTCCAGCCGACAAGAACAAGCGGCAAGATGAGCTAATCGTCCTCAACGTGAG TGGCCGTCGGTTCCAGACCTGGAGGACCACTCTGGAGCGCTATCCCGACACCCTGCTGGGTAGCACAGAG AAGGAGTTCTTCTTCAACGAGGACACCAAGGAGTACTTCTTTGACCGTGACCCGGAAGTGTTCCGTTGTG TGCTCAACTTCTACCGCACTGGGAAGCTGCACTACCCACGCTATGAATGCATCTCTGCCTACGACGATGA GCTGGCCTTCTATGGCATCCTCCCTGAGATCATTGGCGACTGCTGCTATGAGGAGTACAAAGACCGCAAG CGGGAGAACGCTGAGCGGCTCATGGATGACAATGAGTCTGAGAACAACCAGGAGTCCATGCCCTCCCTCA GCTTCCGCCAGACCATGTGGCGGGCCTTCGAGAACCCACACACCAGCACCCTGGCACTGGTCTTCTACTA CGTGACAGGCTTCTTCATTGCGGTCTCGGTGATCACCAACGTGGTGGAGACGGTGCCATGCGGCACGGTG CCTGGGAGCAAGGAACTGCCGTGTGGAGAGCGCTACTCCGTGGCTTTCTTCTGCCTGGACACTGCGTGTG TCATGATCTTCACGGTGGAGTACCTCCTCCGACTCTTCGCGGCACCCAGCAGGTACCGCTTCATCCGCAG TGTGATGAGCATCATCGACGTGGTGGCCATCATGCCCTATTACATTGGCCTGGTCATGACCAACAACGAG GACGTGTCCGGGGCATTTGTCACACTCCGGGTCTTCCGCGTCTTCAGGATCTTCAAGTTCTCCCGACATT CCCAGGGCCTACGGATCCTAGGCTACACCCTGAAGAGCTGTGCCTCAGAACTAGGCTTTCTTCTCTTCTC CCTCACCATGGCCATCATCATCTTTGCCACTGTGATGTTTTATGCTGAGAAGGGCTCCTCCGCCAGCAAG TTCACAAGCATCCCTGCGTCTTTCTGGTACACCATAGTCACCATGACAACACTGGGGTATGGAGACATGG TGCCTAAGACAATTGCTGGGAAGATATTTGGCTCCATCTGCTCCCTAAGCGGCGTCCTGGTCATTGCTCT GCCAGTCCCCGTCATAGTCTCCAACTTTAGCAGGATCTACCATCAGAACCAGAGAGCAGATAAACGCAGG GCACAGAAGAAGGCCCGCCTTGCCAGAATCCGTGTGGCCAAAACAGGGAGCTCCAATGCCTACCTGCACA GCAAGCGCAATGGACTCCTCAATGAAGCTCTGGAGCTGACCGGCACCCCAGAAGAGGAGCACATGGGCAA GACCACGTCACTCATCGAGAGCCAGCACCACCACCTGCTACACTGCTTAGAAAAGACCACTAACCACGAG TTTATTGATGAGCAGATGTTTGAGCAGAACTGCATGGAGAGCTCAATGCAGAACTACCCATCCACCAGAA GCCCTTCTCTGTCCAGCCACTCGGGCCTCACCACCACCTGCTGCTCCCGCCGTAGCAAGAAGACCACGCA CCTGCCCAACTCCAACCTGCCGGCCACCCGCCTGCGCAGCATGCAAGAGCTCAGCACCATCCACATCCAG GGCAGTGAGCAGCCCTCCCTCACCACTAGTCGCTCCAGCCTTAATTTGAAAGCAGACGACGGACTGAGAC CAAACTGCAAAACATCCCAGATTACAACAGCCATCATCAGCATCCCTACTCCCCCAGCTCTAACCCCAGA GGGAGAAAGTCGGCCACCCCCTGCCAGCCCAGGTCCCAACACGAACATTCCTTCCATAACCAGCAATGTT GTCAAGGTCTCTGCCTTGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001270963
Insert Size	1911 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001270963.1, NP_001257892.1
RefSeq Size	6761 bp
RefSeq ORF	1911 bp
Locus ID	65195
UniProt ID	Q62897
Gene Summary	voltage-gated K+ channel that generates an A type K+ current [RGD, Feb 2006] Transcript Variant: This variant (2, also known as Kv4.3M and Kv4.3a) lacks an in-frame exon in the 3' coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1. Sequence Note: The RefSeq transcript was derived from the reference genome assembly. The genomic coordinates were determined from transcript alignments.

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