Dglucy (NM_001173557) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	RGD1311756
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217008 representing NM_001173557 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTACCCTTCTCACTTCTCTTGAGGTCTTGTCTCCCCTCTGCCATAAGGAGTCTACCGAAGGCAGCAC ACATCAGAAACATATCCAGCATGACTGGAGGACTCCAGCCGGCTAGTGTGGTGGTCCTGCCCAGATCCCT AGCACCAGCCTTTGAAAGCTTCTGCCAGGGTAACCATGGTCCTCTACCCCTACTCGGACAAAGTGAGCCG GAGAAGACATTTCCTCAGCTGAGTGCTGTTCCAGACATAAGGACCATCTGTCCACAGTTTCAGAAATACG AGTTTGGCACCTGCACTGGCATCCTGACCTCACTGGAAGAGTACTCAGAACAGCTCAAGGACATGGTGAC CTTCATCATGGACTGCAGCTTCTCTATAGAAGAGGCCTTGGAACAGGCAGGGATCCCCAGAAGAGACCTA ACAGGTTCCGGCCATGCAGGAGCATACAAGACAGCAGTACCCTGTGCCACCATCGCTGGCTTCTGCTGCC CTCTGGTGGTCACAATGAGACCAGTTCCCAAGGACAAGCTGGAAAGGCTGTTGCTGGCCACTCACTCCCT GGGAGGACAGCAAGGACAACCCATCCACATTGGTGACCCAGAACTTCTGGGAATCAAGCCACTTTCTAAA CCTGACTATGGGAGTTATGTGGAGTGTCGGCCCGGGGATGTCCCAGTGTTCTGGCCTTCTCATCTGACCA GTCTGGAAGCAGTCATCGGCTGCAAGGCCCCGTTGGCTTTCGCCAGCCCTCCAGGCTGCACGGTGATGAT CCCCAGGGACACAGTGTCCCCAGCCAGTCGTCCGACTCCTCAGATGGTTCCAGAAGTCCACGCCATCTCC AAAGACCCTTTGCACTACAGCATAGTGTCGGCCCCTGCTGCTCAAAAGATCAGAGAGCTAGAGTCCACAA TTGCCACAGACCCAGGTAACCGGGGAATTGGGCACCTGCTACTTAAAGATGAGTTACTGCTGGCAGCTCT GTCACTGTCTCACGCGCACTCCGTACTGGTCACCACTGGATTCCCGACACATTTCAGTCACGAGCCCCCA GAAGAGACGGATGGCCCACCGGGAGCTATCGCTTTAGCTGCCTTCCTACAGGCTCTGGGGAAGGAGGCCG CCATGGTGGTAGACCGGAGAGCCTTGAACTTGCATACGAAGATTGTTGAAGATGCCGTGAAGCAAGGAGT TCTCAAGACACCAATCCCCATATTAACTTACCGAGGAGGATCCGTGGAAGATGCTCGGGCATTTCTGTGC AAAGATGGGGACCCCAAGTCTCCTAGATTCGACCATCTGGTGGCCGTAGAGCGTGCGGGAAGGGCCGCGG ATGGTAATTACTACAACGCAAGGAAGGTGAACATCAAACACTTAGTTGACCCCATCGACGACATTTTCCT GGCCGCACAAAAGATTCCTGGAATCTCATCAACTGGCGTTGGTGATGGAGGCAATGAGCTCGGGATGGGC AGAGTGAAGGAGGCCGTGAAGAAGCACATCAGAAATGGAGATGTCATCGCCTGTGATGTGGAGGCTGACT TTGCCGTCATTGCTGGTGTTTCTAACTGGGGAGGCTATGCCCTGGCCTGTGCACTATACATTCTGAACTC ATGCCCAGTCCATGAGCGTTACCTGAGGAGGGCAACTGGACCTTCCGGGGAAGCAGGGGAACAAAGCTGG ATTCAGGCCCTGCCGTCTGTCGCTAAGGAAGAAAAAATGTTGGGCATCCTGGTACAGAACCAAGTTCGAA GTGGAGTCTCGGGCATCGTGGGCATGGAAGTGGATGGGTTGCCTTTCCATGGTGTCCACGCTGAGATGAT CCAGAAGCTGGTGGATGTCACCACAGTGCCCATGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001173557
Insert Size	1857 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001173557.1, NP_001167028.1
RefSeq Size	3102 bp
RefSeq ORF	1857 bp
Locus ID	362769

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