Riok1 (NM_001100511) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN216922 representing NM_001100511 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACTGCAGTCTGGATAGGATGGCCACGGTGGTCCCCGGGCAATTTGACGACGCGGATTCCTCAGACA GTGAAGACAAAGAAGTGCAGTCAATCCAAGCAAAAGACGACATTCTACTTAAAAACCTTCAGAATGATGC AGCAGAGGTGAAGGGCGAAGATGAGATTGAGGAGGAAGACAGCTATGATTATGACAGTGACTGGTACTTG GACGACTCGACTGGAAAACTCACCAAGGGTTGGACCTGGAATGGCAGCAGTAACCATCAGGCAAATCGAC AGACTTCTAACTACAATTCAGCCAAAATGTCTACTCCAATTGACAAGTCTTTGCGAAAATTTGAAAATAA GATCAATCTAAGTAAGCTGAATGTTACTGATTCTGTCATAAATAAAGTCACCGTAAAATTGAGACAAAAA GAAGCAGAATCGTATCGCATTAAAGACAAGGCCGACAGAGCAACTGTAGAACAGGTTTTGGATCCCAGAA CAAGGATGATTTTATTCAAGTTGTTGCATAAAGAGCACATATCAGAGATACATGGCTGCATCAGTACTGG AAAAGAAGCTAATGTATACTATGCTAGCACACCCAGTGGAGAGAGCAGAGCAATCAAAATATACAAAACT TCTATTTTGATGTTCAAAGATCGTGATAAGTATGTGACTGGGGAGTTCAGGTTCCGCCGTGGCTATTGCA AAGGAAACCCTAGAAAAATGGTGAAGACGTGGGCAGAGAAGGAGATGAGGAATTTGTCCAGGTTAAGAAC AGCAAACATACCATGCCCAGAACCAATCAGGCTAAGAAGTCATGTTCTCCTCATGAGCTTCATTGGCAAG GATGACATGCCTGCACCACTTTTGAAAAATGTCCAGTTGTCAGAATCCAAGGCACGGGAGCTGTACCTGC AGGTCATTCAGTACATGAGGAAAATGTATCAGGATGCCAGACTTGTCCATGCAGATCTCAGTGAATTCAA CATGCTGTACCATGGTGGAGATGTATATATCATTGATGTTTCTCAGTCTGTGGAGCATGACCACCCACAT GCATTGGAGTTCTTGAGAAAAGACTGTGCCAATGTCAATGATTTCTTTTTCAAGCATGCTGTTGCTGTGA TGACTGTGAAGGAGCTCTTCGAGTTCGTCACGGATCCCTCCATCACCCTTGAGAACATAGATGCTTATTT GGAAAAGGCTATGGAAATAGCATCTCAGAGGACCATGGAAGAAAAGACTAGCCAAGATCATGTGGATGAA GAGGTGTTCAAACAAGCATATATTCCCAGAACCTTAAATGAAGTAAAGAATTATGAGAGAGATGTGGACA TAATGATGAGGTTAAAGGAAGAGGACATGGCTTTGAACACTCAGCAGGACAATATTCTATACCAGACTGT CACAGGACTGAAAAAAGATTTGTCAGGAGTCCAGAAGGTCCCTGCGCTCCTAGAAAGTGAAGTTAAGGAA GAGACTTGTTTTGATTCAGACGATGCTGGGAGCTCTGAGTGCTCTGACACAGACCCTGAAGAGCAGGGAG ATCAAGCCGGATGCCAAAACCATACTGCTGACCCTGACGTTGATAAAAAGGAAAGAAAAAAGATGGTCAA GGAAGCCCAGAGAGAGAAGAGAAAAAATAAAATCCCTAAACACGTGAAAAAAAGAAAGGAAAAAACAGCC AAGGCCAAAAAGGGCAAATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001100511
Insert Size	1701 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001100511.1, NP_001093981.1
RefSeq Size	2085 bp
RefSeq ORF	1701 bp
Locus ID	291061

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