St6gal2 (NM_001100888) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	ST6Gal-II; ST6GalII
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN216848 representing NM_001100888 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAACCACACTTGAAGCAATGGCGACAACGAATGCTCTTTGCAATATTTGTTTGGGGGCTCCTCTTTT TGGCAATTTTCATCTATTTCACCAACAGCAATCCTGCTGCACCTATGCCCAGCTCCTTTTCCTTCCTGGA GAGCCGCGGGCTCCTGCCTGTGCAGGGCAAGCAGAGGGTCATCATGGGTGCATTGCAGGAGCCCTCTTTG CCCAGAAGTTTGGAGCCAAGCAAAGTACTGATGGACGGCCACTCTGCGAGCCCTTTCAACAGCTGGCCTG GGGACCCACAGAAAGGGGACCAGGCCCAAGATGGCTTTGACAATGGGGATGAGTTTTTTACATCCCAGGT CGGGAGGAAATCACAAAGTGCTTTCTATCCTGAGGAAGACAACTACTTTTTTGTTGCGGGTCAGCCTGGG TTGTACCACCACCGGCAGGGTGCCCTGGGGCTGCCATCTCCGGGGGAGTCCTCATGGCAATCAGGACCTG GTCAGCCCAAGCAGGAGAAGCTGCGTCACCCAAGGAGAGGCAGTTTGCCTGAGGAAGCCTATGATAGTGA CATGCTGTCGACCTCCATGTCCAGAGCCTTCCTGTACCGGCTCTGGAAGGGAACCGTGTCCTCTAAGATG TTGAACCCGCGCCTGCAGAAAGCCATGCGCTACTACATGTCCTTTAACAAGCACGGTGTGCGCTTCAGCA GGCGGGGGCGGCGTGAGGCACGACGTACAGGGCCAGAGCTGCTGTGCGAGATGCGCAAGCGTGTGCGTGT GCGCACCTTGGACGGCAAAGAGGCGCCCTTCTCAGGGCTGGGCTGGCGGCCTCTGGTACCTGGGGTCCCT CTGAGCCAGTTGCACCCCCGCGGTTTGCGCAGCTGCGCAGTTGTAATGTCTGCCGGAGCCATCCTGAACT CCTCCTTGGGGGAGGAAATCGATTCTCATGATGCAGTTTTGAGATTTAACTCTGCCCCTACACGTGGCTA TGAGAAAGATGTCGGAAATAAAACCACAGTACGCATCATTAATTCTCAGATTCTGGCCAACCCCAGCCAT CACTTCATTGACAGTTCTTTATATAAAGATGTTATTCTGGTAGCCTGGGATCCAGCTCCTTATTCTGCCA ATCTTAACCTGTGGTATAAGAAGCCAGATTACAACCTTTTCACTCCATATATCCAGCATCGCCTGAAATA CCCAACTCAGCCATTTTACATTCTTCACCCCAAATTCATATGGCAGCTTTGGGACATTATCCAGGAAAAC ACAAGGGAGAAGATACAGCCCAACCCACCATCTTCTGGTTTTATTGGAATCCTCGTCATGATGTCCATGT GCCAAGAGGTGCACGTGTATGAGTACATCCCATCTGTCAGACAGACAGAACTTTGCCACTACCATGAGCT GTACTACGACGCAGCCTGCACCTTGGGAGCCTACCACCCATTGCTTTATGAAAAGCTACTGGTGCAGCGC CTTAACACAGGCACCCAGGCAGATTTGCACCACAAGGGAAAGGTGGTCCTGCCAGGCTTCCAGACTCTGC GGTGCCCAGTAACCAGGCCCAACAATACAAACACTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001100888
Insert Size	1578 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001100888.1, NP_001094358.1
RefSeq Size	1800 bp
RefSeq ORF	1578 bp
Locus ID	301155
UniProt ID	Q701R3
Gene Summary	Transfers sialic acid from the donor of substrate CMP-sialic acid to galactose containing acceptor substrates. Has alpha-2,6-sialyltransferase activity toward oligosaccharides that have the Gal-beta-1,4-GlcNAc sequence at the non-reducing end of their carbohydrate groups, but it has weak or no activities toward glycoproteins and glycolipids.[UniProtKB/Swiss-Prot Function]

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