Igf2bp2 (NM_001270599) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	RGD1305614
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN216841 representing NM_001270599 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGTTGATTACTCAGTCTCTAAAAAGCTAAGGAGCAGAAAAATCCAGATTCGAAACATCCCGCCTC ACCTGCAGTGGGAGGTGTTGGATGGCCTGTTGGCTGAGTATGGGACGGTAGAGAACGTGGAGCAAGTCAA CACGGACACAGAAACAGCCGTTGTCAACGTCACCTATATGACAAGGGAAGAGGCAAAGGTAGCTATTGAG AAGTTAAGTGGGCATCAGTTCGAGGACTACTCCTTCAAGATTTCCTACATCCCCGACGAAGAATTGAGCT CCCCTTCACCCCCTCATCGAGCCCGGGAACAAGGCCATGGCCCCGGGAGCTCTTCTCAGGCCAGACAGAT TGACTTCCCGCTGAGGATCCTGGTCCCCACCCAGTTTGTTGGTGCCATCATCGGAAAGGAGGGCTTGACC ATAAAGAACATCACTAAACAGACCCAGTCCCGGGTAGACATCCACAGGAAGGAGAACTCTGGGGCTGCCG AGAAGCCTGTCACAATCCACGCCACCCCAGAAGGGACATCTGAAGCCTGCCGCATGATTCTTGAGATTAT GCAGAAAGAGGCTGATGAGACCAAACTGGCAGAAGAGGTTCCTTTGAAAATCTTGGCCCACAACGGCTTC GTCGGAAGACTGATTGGCAAAGAAGGCAGAAACCTGAAGAAAATTGAACACGAGACAGGGACCAAGATAA CTATCTCATCCTTGCAGGATTTGAGCATATATAACCCCGAGAGAACCATCACGGTGAAAGGTACCATTGA AGCCTGTGCCAGTGCTGAGATGGAGATTATGAAGAAGCTACGCGAGGCCTTTGAAAACGACATGCTGGCC GTTAATCAACAAGCCAATCTGATCCCAGGGCTGAACCTCAGTGCACTCGGCATCTTTTCGACAGGACTGT CTGTGCTTCCTCCACCAGCAGGGCCCCGTGGAGCTCCCCCGAGTCCCCCCTATCACCCATTTGCTACCCA CTCCGGATACTTCTCCAGTCTGTACCCTCATCACCATTTCGGCCCATTCCCGCATCACCACTCGTACCCA GAGCAAGAGACGGTGAGTCTCTTCATCCCAACGCAGGCTGTGGGTGCTATCATCGGGAAAAAGGGGGCAC ACATCAAACAGCTGGCTCGATTTGCCGGTGCCTCCATCAAGATCGCTCCAGCGGAAGGTCCAGATGTCAG TGAGAGGATGGTCATCATCACCGGGCCTCCTGAAGCCCAGTTCAAGGCTCAGGGACGGATCTTTGGGAAA CTGAAAGAAGAAAACTTCTTTAATCCCAAAGAAGAGGTGAAGCTGGAGGCCCACATCCGAGTTCCCTCCT CGACAGCTGGCCGGGTGATTGGCAAAGGCGGTAAAACCGTAAATGAGCTGCAGAACTTGACAAGTGCAGA AGTTATAGTGCCTCGTGACCAAACGCCAGATGAGAACGAGGAAGTGATCGTCAGAATTATCGGGCATTTT TTTGCTAGCCAGACTGCACAACGCAAGATCAGGGAAATTGTACAGCAGGTGAAGCAGCAGGCGCAGAAAT ACCCTCAGGGAGTCGCCCCACAGCGCAGCAAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001270599
Insert Size	1575 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001270599.1, NP_001257528.1
RefSeq Size	3629 bp
RefSeq ORF	1575 bp
Locus ID	303824
Gene Summary	The protein encoded by this gene is a member of a family of mRNA binding proteins that have been shown to bind to several different mRNAs in mammalian cells, including insulin-like growth factor 2 mRNA. Generation of two different isoforms by alternative translation initiation at an internal start codon, has been described for this gene (PMID:22427968). [provided by RefSeq, Jul 2012] Transcript Variant: This variant (1) uses an in-frame downstream start codon, and encodes a shorter isoform (2) missing N-terminal 68 aa compared to isoform 1. It lacks a conserved N-terminal RNA Recognition Motif (RRM) and is predicted to differ functionally from the full-length isoform (PMID:22427968). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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