Gps1 (NM_001270939) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	MFH
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN216777 representing NM_001270939 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGCTGCCGGTTCAGGTGTTTAACTTGCAGGGGGCCGTGGAACCCATGCAGATTGATGTAGATCCGC AGGAAGATCCCCAGAACGCACCTGATGTCAACTACGTGGTGGAGAACCCCACCCTGGATCTGGAGCAGTA TGCAGCCAGCTACAGTGGTCTGATGCGTATTGAGCGGCTACAGTTTATTGCTGACCGCTGCCCTCCGTTG CGAGTGGAGGCCTTGAAAATGGCTCTGTCCTTCGTGCAGAGGACCTTCAATGTGGACATGTATGAAGAGA TACACCGGAAGCTCTCTGAGGCTACCAGGGAGCTGCAGAATGCACCTGATGCCATCCCTGAGAGTGGAGT GGAGCCCCCACCCCTGGACACAGCCTGGGTGGAGGCCACTCGGAAGAAGGCCCTGTTGAAACTGGAGAAG TTAGACACGGACTTGAAGAACTACAAGGGCAATTCTATCAAGGAGAGCATCAGGCGCGGCCATGATGACC TGGGTGACCACTACCTGGACTGTGGGGACCTCAGCAATGCCCTCAAGTGTTATTCGCGAGCCCGAGACTA CTGCACCAGTGCTAAGCATGTCATCAACATGTGCCTCAATGTCATCAAGGTCAGTGTCTACTTGCAGAAT TGGTCTCATGTGTTAAGCTATGTCAGCAAGGCTGAGTCTACTCCAGAGATTGCTGAGCAGCGGGGAGAGC GGGACAGCCAGACTCAAGCCATCCTCACCAAGCTCAAGTGTGCTGCAGGCTTGGCTGAGCTGGCTGCACG AAAGTATAAGCAAGCTGCCAAGTGCTTCCTTCTGGCTTCCTTTGATCACTGTGATTTCCCAGAGCTGCTG TCCCCCAGCAATGTGGCTGTCTATGGTGGCCTATGTGCCTTAGCCACCTTTGACCGGCAGGAGCTGCAAC GCAATGTCATCTCCAGCAGCTCCTTCAAGTTGTTCCTGGAGCTGGAGCCACAGGTCAGAGACATCATCTT CAAATTTTATGAGTCCAAGTATGCCTCATGCTTGAAGATGTTGGACGAGATGAAGGACAACCTGCTCCTG GACATGTACCTGGCCCCTCACGTCAGGACACTGTATACCCAGATCCGTAACCGGGCTCTCATCCAATACT TCAGCCCCTATGTGTCAGCTGATATGCACAAGATGGCTGCTGCCTTCAACACAACAGTTGCAGCTCTGGA GGATGAGCTCACACAGCTCATCCTGGAGGGCCTTATTAATGCCCGCATCGACTCACACAGCAAGATACTG TATGCTCGAGACGTGGATCAGCGTAGCACCACCTTTGAGAAGTCCCTGCTGATGGGCAAGGAGTTCCAAC GACGGGCCAAAGCCATGATTCTGAGAGCAGCTGTGCTACGCAACCAGATCCATGTCAAGTCTCCTCCTAG AGAAGGGAGCCAAGGGGAGCTGACTCCAGCAAACAGCCAGTCACGGATGAGCACCAACATGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001270939
Insert Size	1464 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001270939.1, NP_001257868.1
RefSeq Size	1863 bp
RefSeq ORF	1464 bp
Locus ID	117039
UniProt ID	P97834
Gene Summary	human homolg suppresses G-protein and mitogen-activated protein kinase mediated signal transduction [RGD, Feb 2006] Transcript Variant: This variant (2) has an alternate 5' exon, com pared to variant 1. The resulting isoform (2) is shorter and has a distinct N-terminus, compared to isoform 1.

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