Ddc (NM_001270852) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	AADC
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN216763 representing NM_001270852 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATTCCCGTGAATTCCGGAGAAGAGGGAAGGAGATGGTGGATTATATAGCTGACTATCTGGACGGCA TTGAGGGACGTCCAGTGTACCCTGACGTGGAGCCTGGCTACCTTCGGGCCCTGATCCCCACCACTGCCCC CCAGGAGCCAGAAACATATGAGGACATAATCAGAGACATTGAAAAGATAATCATGCCAGGGGTCACACAC TGGCACAGCCCCTACTTCTTCGCTTACTTCCCCACGGCCAGCTCCTACCCAGCTATGCTTGCGGACATGC TGTGCGGGGCTATCGGCTGCATTGGCTTCTCCTGGGCTGCAAGCCCAGCATGCACAGAGCTGGAGACAGT GATGATGGATTGGCTGGGGAAGATGCTTGAGCTGCCAGAGGCCTTTTTGGCTGGAAGAGCTGGGGAAGGG GGAGGAGTGATCCAGGGAAGTGCCAGCGAAGCCACCTTGGTGGCCCTACTGGCTGCTCGGACTAAAATGA TCCGCCAGCTGCAGGCAGCCTCCCCAGAGCTGACACAAGCTGCTCTCATGGAAAAGCTTGTCGCTTACAC ATCTGATCAGGCACATTCCTCCGTAGAAAGAGCTGGATTAATTGGTGGAGTCAAAATAAAAGCAATTCCT TCAGATGGCAACTACTCCATGAGAGCTGCTGCCCTTCGGGAGGCCCTGGAGAGAGACAAGGCGGCTGGCC TGATTCCTTTCTTCGTGGTTGTCACCCTAGGAACCACATCTTGCTGCTCTTTTGACAATCTCCTAGAAGT GGGTCCCATCTGCAACCAGGAGGGTGTATGGCTGCACATTGATGCTGCATACGCAGGCAGTGCCTTTATC TGTCCTGAGTTCCGGTATCTTCTGAATGGCGTGGAGTTTGCAGATTCCTTTAACTTTAATCCCCACAAGT GGCTTTTGGTGAATTTTGACTGCTCTGCCATGTGGGTGAAGAAGAGAACTGACCTAACCGAAGCCTTTAA TATGGACCCTGTTTATCTGAGGCACAGTCACCAGGACTCAGGACTCATCACTGACTACAGGCACTGGCAA ATCCCACTGGGGCGAAGATTTCGCTCCCTGAAAATGTGGTTTGTTTTTAGAATGTACGGAGTCAAGGGGC TGCAGGCTTACATTCGAAAGCACGTGAAGCTGTCTCATGAGTTTGAGTCCCTGGTACGCCAGGACCCTCG CTTTGAAATTTGCACGGAAGTCATCCTCGGGTTGGTCTGCTTCCGGCTAAAGGGCTCCAACCAGTTGAAC GAAACTCTCTTACAAAGAATAAACAGCGCCAAAAAAATCCACTTGGTTCCGTGTCGTCTCCGAGACAAGT TTGTGCTGCGCTTTGCGGTGTGCTCCCGCACTGTGGAGTCTGCCCACGTGCAGCTGGCCTGGGAGCACAT CCGAGATCTAGCGAGCAGTGTGCTGAGGGCAGAGAAAGAGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001270852
Insert Size	1443 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001270852.1, NP_001257781.1
RefSeq Size	5094 bp
RefSeq ORF	1443 bp
Locus ID	24311
UniProt ID	P14173
Gene Summary	catalyzes the decarboxylation of L-5-hydroxytryptophan to serotonin and L-3,4-dihydroxyphenylalanine to dopamine [RGD, Feb 2006] Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1 and 3 encode the same protein (isoform a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.