Alkbh5 (NM_001191643) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	RGD1309496
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN216557 representing NM_001191643 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCCGCCAGCGGCTACACGGACCTGCGTGAGAAGCTCAAGTCCATGACGTCCCGGGACAACTACA AGGCAGGCAGCCGGGAGGCCGCCGCCGCTGCAGCCGCTGCTGTGGCCGCCGCCGCGGCTGCCGCGGCCGC CGCCGAGCCTTACCCGGTGTCCGGGACCACCAAACGGAAGTACCAGGAGGACTCAGACCCCGAACGCAGT GACTACGAGGAGCATCAGTTGCAGAAGGAGGAGGAGGCGCGCAAGGTGAAGAGCGGCATCCGGCAGATAC GTCTCTTCAGCCAGGATGAGTGCTCCAAGATCGAGGCCCGCATCGACGAGGTGGTGTCCCGCGCTGAGAA GGGCCTGTACAACGAGCACACTGTGGATCGGGCCCCCCTGCGCAACAAGTACTTCTTCGGCGAGGGCTAC ACGTACGGTGCCCAGCTGCAAAAGCGCGGGCCAGGCCAGGAGCGCCTCTACCCGCCAGGCGACGTCGATG AGATCCCGGAATGGGTGCATCAGCTGGTGATCCAGAAGCTGGTGGAGCACCGCGTCATCCCCGAGGGCTT CGTCAACAGCGCAGTCATCAACGACTACCAGCCTGGCGGCTGCATCGTATCTCACGTAGACCCCATCCAC ATCTTCGAGCGCCCGATCGTGTCCGTGTCTTTCTTTAGCGACTCGGCACTTTGCTTCGGTTGCAAGTTCC AGTTCAAGCCCATCCGGGTGTCGGAACCTGTGCTTTCCCTGCCGGTGCGCAGGGGGAGCGTGACTGTGCT CAGTGGGTATGCTGCTGATGAGATCACTCACTGCATACGGCCTCAGGACATCAAAGAACGCCGGGCGGTC ATCATTCTCAGGAAGACAAGATTAGATGCACCACGATTGGAAACAAAATCCCTGAGCAGCTCCACATTGC CACCCAGCTATGCTTCAGATCGTCTGTCAGGAAACACCAGAGACCCTGCGCTGAAACCCAAAAGGTCCCA CCGCAAGGCAGACCCTGATGCTGCCCATAGGCCCCGGATCCTGGAAATGGACAAAGAAGAAAACCGGCGG TCTGTGCTTCTGCCCACACACCGGCGGAGAGGGAGTTTTAGCTCTGAGAACTATTGGCGCAAGTCCTATG AGTCCTCAGAAGATTGCCCAGAGGCAGCCAGCAGCCCCACCCGCAAGGTGAAGATGAGGAGACACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001191643
Insert Size	1188 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001191643.1, NP_001178572.1
RefSeq Size	1755 bp
RefSeq ORF	1188 bp
Locus ID	303193
UniProt ID	D3ZKD3
Gene Summary	Dioxygenase that demethylates RNA by oxidative demethylation: specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes (By similarity). Can also demethylate N(6)-methyladenosine in single-stranded DNA (in vitro). Requires molecular oxygen, alpha-ketoglutarate and iron. Demethylation of m6A mRNA affects mRNA processing and export (By similarity). Required for the late meiotic and haploid phases of spermatogenesis by mediating m6A demethylation in spermatocytes and round spermatids: m6A demethylation of target transcripts is required for correct splicing and the production of longer 3' UTR mRNAs in male germ cells (By similarity).[UniProtKB/Swiss-Prot Function]

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