Poglut1 (NM_001100652) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Ktelc1; RGD1306248
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN216548 representing NM_001100652 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCGGCTGTCGGGCTGTAGGCTTAGGCCATGGATGCTGTTATTGCTCCTGTTCCCGGTGCAGGGCC GCCAGAAGGACTCAGGCTCAAAATGGAAAGTATTTATTGACCAAATTAACAGGGCTTTGGAGAATTATGA ACCATGTTCAAGTCAAAACTGCAGCTGCTATCACGGGGTCATAGAAGAGGATCTGACTCCTTTCCGAGGT GGAATCTCCAGGAAGATGATGGCCGAGGTGGTCAGACGGAGGTTAGGAACCCACTACCAGATCATTAAGC ACAGATTATTCAGGGAAGACGACTGCATGTTTCCCTCCAGGTGTAGTGGTGTAGAACACTTTATTTTGGA AGTGATCCGTCGCCTCCCTGACATGGAAATGGTAATCAATGTCCGAGACTATCCTCAGGTTCCTAAGTGG ATGGAGCCTACCATCCCTGTCTTCTCCTTCAGTAAGACATCGGAGTACCATGATATAATGTACCCTGCGT GGACATTTTGGGAAGGGGGTCCTGCTGTGTGGCCACTTTATCCTACAGGTCTTGGGCGGTGGGACCTCTT CAGAGAAGATCTGTTAAGGTCAGCAGCACAATGGCCATGGGAAAAGAAGAATTCTACAGCATATTTCCGG GGATCAAGGACAAGCCCTGAAAGAGATCCTCTCATTCTTCTCTCTCGGAAAAATCCAAAACTCGTTGATG CAGAATACACCAAAAACCAGGCCTGGAAGTCTATGAAAGATACTTTGGGAAAGCCAGCCGCTAAGGATGT GCATCTTATAGATCACTGCAAATACAAATACCTGTTTAATTTCCGAGGTGTAGCTGCAAGTTTCCGGTTC AAACACCTCTTCTTGTGTGGCTCGCTGGTCTTCCATGTTGGTGATGAGTGGGTTGAGTTCTTCTACCCAC AGCTAAAGCCCTGGGTTCACTACATCCCAGTCAAAACAGACCTCTCCGATGTCCAGGAGCTGTTGCAGTT TGTGAAAGCAAATGATGATCTTGCTCAGGAAATTGCTAAAAGGGGAAGCCAGTTCATCATAAACCATTTG CAGATGGATGACATCACTTGTTACTGGGAGAACCTCTTGACTGAATATTCCAAATTCCTGTCCTATAATG TAACAAGAAGAAAAGATTATTATCAGATCATTCCCAGACGTTTGAAAACTGAACTGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001100652
Insert Size	1179 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001100652.1, NP_001094122.1
RefSeq Size	2935 bp
RefSeq ORF	1179 bp
Locus ID	288091
UniProt ID	G3V9D0
Gene Summary	Dual specificity glycosyltransferase that catalyzes the transfer of glucose and xylose from UDP-glucose and UDP-xylose, respectively, to a serine residue found in the consensus sequence of C-X-S-X-P-C. Specifically targets extracellular EGF repeats of protein such as CRB2, F7, F9 and NOTCH2 (By similarity). Acts as a positive regulator of Notch signaling by mediating O-glucosylation of Notch, leading to regulate muscle development (By similarity). Notch glucosylation does not affect Notch ligand binding (By similarity). Required during early development to promote gastrulation: acts by mediating O-glucosylation of CRB2, which is required for CRB2 localization to the cell membrane (By similarity).[UniProtKB/Swiss-Prot Function]

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