Agxt (NM_001276706) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	AGT; Spat; SPT
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN216546 representing NM_001276706 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCTCGCACCAGCTGCTGGTGCCACCCCCAGAGGCCCTGAGCAAGCCCCTGTCAATTCCTAAGAGGC TCCTGTTGGGTCCGGGACCCTCCAACCTGGCTCCTCGTGTGCTAGCAGCTGGAAGTCTGAGGATGATTGG CCACATGCAAAAAGAGATGTTTCAGATCATGGATGAGATCAAGCAGGGCATCCAGTATGTGTTCCAGACC AGGAACCCCCTCACACTGGTTGTCAGCGGCTCAGGACATTGTGCCATGGAGACTGCCCTGTTCAACCTCC TGGAGCCTGGGGACTCCTTTCTTGTGGGAACCAATGGCATCTGGGGGATACGGGCTGCAGAGATCGCTGA GCGGATTGGAGCCCGTGTGCACCAGATGATCAAGAAGCCTGGAGAACATTACACACTGCAGGAGGTGGAG GAGGGCCTGGCTCAGCATAAACCAGTGTTGCTGTTCCTGACCCACGGGGAGTCATCCACTGGTGTGCTGC AGCCCCTGGATGGTTTCGGGGAGCTCTGCCACAGGTATCAGTGCCTACTCCTGGTGGACTCGGTGGCATC ATTGGGCGGAGTCCCTATCTACATGGACCAACAAGGCATCGACATCTTGTACTCTGGCTCTCAGAAGGTC CTGAATGCCCCACCAGGGATCTCCCTCATCTCCTTCAACGACAAGGCCAAATCCAAAGTCTACTCCCGGA AGACAAAGCCAGTCTCCTTCTACACAGACATCACTTATTTGTCCAAGTTGTGGGGCTGTGAGGGCAAGAC CAGAGTAATTCATCATACGTTGCCTGTCATCAGCTTATACTGCCTGAGGGAGAGCCTAGCACTCATTTCA GAGCAGGGCCTGGAGAATTCCTGGCGGCGTCACAGGGAGGCTACAGCACATCTGCACAAGTGCCTGCGGG AGTTGGGCTTAAAGTTCTTTGTGAAGGACCCGGAAATCCGGCTACCTACAATCACCACCGTGACCGTGCC TGCCGGCTACAACTGGAGGGACATCGTCAGCTACGTGCTGGACCACTTCAACATTGAAATCTCTGGTGGT CTTGGGCCCTCTGAGGATAAGGTGCTGCGGATTGGCCTCCTGGGCTACAACGCCACCACAGAGAATGCGG ACCGTGTAGCGGAGGCCCTGAGGGAGGCCCTGCAACATTGTCCTAAGAATAAATTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001276706
Insert Size	1179 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001276706.1, NP_001263635.1
RefSeq Size	1628 bp
RefSeq ORF	1179 bp
Locus ID	24792
UniProt ID	P09139
Gene Summary	This gene encodes alanine-glyoxylate aminotransferase, which catalyzes the interconversion of L-alanine and glyoxylate to pyruvate and glycine. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. The longer transcript variant includes an upstream translation start codon and a downstream translation start codon. The upstream start codon initiates the translation of the mitochondrial enzyme precursor while the downstream start codon initiates the translation of the peroxisomal enzyme (see PMID:2332438). [provided by RefSeq, Feb 2013] Transcript Variant: This variant (1) represents the longer transcript. It contains an upstream start codon and a downstream start codon. Isoform 3, also known as the peroxisomal enzyme, is encoded from the downstream start codon, which is identical to the mature peptide of isoform 1, also known as the mitochondrial enzyme.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.