Sphk1 (NM_001270810) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN216533 representing NM_001270810 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAACCAGCAGACTGTCCCCGGGGACTGCTCCCACGGCCATGCAGAGTGTTGGTGCTGCTGAACCCCC GGGGTGGCAAGGGCAAGGCTCTGAAGCTCTTTCAGAGCCGTGTGCGGCCCCTTCTGGAGGAGGCTGAGGT ATCCTTTAAACTGATGCTCACCGAACGGCAGAACCATGCCAGGGAGCTGGTGTGTGCAGAAGAACTGGGT CACTGGGATGCCTTGGCGGTCATGTCCGGTGACGGTCTGATGCACGAGGTGGTGAATGGGCTAATGGAAC GACCTGACTGGGAGAGTGCCATCCAGAAACCCCTATGTAGCCTCCCTGGAGGCTCTGGCAATGCGCTGGC AGCTTCTTTGAACTACTATGCTGGGCACGAGCAGGTCACTAATGAAGATCTCCTGATCAACTGCACACTG CTGTTATGCTGCCGGCAGCTGTCACCCATGAATCTGCTGTCCCTGCACACCGCTTCCGGACGGCAACTCT ATTCTGTTCTCAGTCTGTCCTGGGGTTTCGTTGCTGACGTGGACTTGGAGAGTGAGAAATACAGGAGCTT GGGGGAGATTCGTTTCACGGTGGGAACCTTTTTTCGCCTAGCAAGCCTGCGCATTTACCAAGGCCAACTG GCCTACCTTCCTGTAGGGAAGGCTGCCTCTAAGATACCTGCTTCCTCACTGGCACAGAAGGGCCCTGCCA ACACATACCTCGTTCCTCTGGAGGAGCCAGTGCCACCTCATTGGACTGTGGTGCCAGAGCAGGACTTCGT TCTGGTGCTGGTGCTGCTTCACACCCACCTGAGCACAGAGATGTTTGCAGCACCCATGGGCCGCTGCGAG GCTGGTGTTATGCATCTGTTCTATATACGTGCGGGGGTGTCAAGGGCCATGCTGCTGCGCCTCTTCCTGG CCATGCAGAAGGGCAAGCATATGGACCTCGACTGCCCGTACTTGGTTCATGTGCCAGTGGTTGCCTTCCG CCTGGAACCCAGGAACCAGAGGGGAGTGTTTTCTGTGGACGGGGAGCTGATGGTGTGTGAAGCTGTGCAG GGCCAAGTGCACCCAAACTACCTTTGGATGGTCTCTGGTAGCAGTGACTCCCCGTCCGGCCGGGACTCCC AGCGGAGGCCACCTCCAGAAGAACCAATATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001270810
Insert Size	1152 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001270810.1, NP_001257739.1
RefSeq Size	2565 bp
RefSeq ORF	1152 bp
Locus ID	170897
UniProt ID	Q91V26
Gene Summary	catalyzes the conversion of sphingosine to sphingosine 1 phosphate, which plays a role in cell proliferation and differentiation [RGD, Feb 2006] Transcript Variant: This variant (5) uses an alternate splice junction at the 3' end of the first exon compared to variant 1. The resulting isoform (b) is shorter at the N-terminus compared to isoform a. Variants 2, 3, 4, 5, and 6 all encode the same isoform (b). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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