Acp5 (NM_001270889) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Trap; TTRRAP
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN216372 representing NM_001270889 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATACGTGGATGGTGCTGCTGGGCCTGCAAATCCTGCTGCTCCCACTCCTGGCTCACTGTACAGCCC CAGCTTCCACCCTGAGATTCGTGGCTGTGGGTGACTGGGGAGGGGTCCCCAATGCCCCATTCCACACAGC CCGGGAAATGGCCAATGCTAAAGAAATCGCCAGAACCGTGCAGATTATGGGCGCTGACTTCATCATGTCC CTGGGGGACAACTTCTACTTCACTGGAGTGCATGACGCCAATGACAAGAGGTTCCAGGAGACCTTTGAGG ATGTGTTCTCTGACCGTGCCCTCCGCAACATCCCCTGGTACGTGCTGGCTGGAAACCATGATCACCTTGG CAATGTCTCGGCACAAATTGCCTACTCCAAGATCTCCAAGCGCTGGAACTTCCCCAGCCCTTATTACCGT TTGCGTTTCAAAGTTCCGCGGTCGAACATCACCGTGGCCATCTTTATGCTGGACACCGTGATGCTGTGTG GCAATTCCGACGACTTTGTCAGCCAGCAGCCTGAAATGCCCCGAGACCTGGGAGTGGCCCGCACTCAGCT GTCCTGGCTCAAAAAACAGTTGGCAGCAGCCAAGGAGGACTATGTTTTGGTGGCCGGTCACTACCCCATC TGGTCCATCGCCGAGCACGGACCCACCCGCTGCCTGGTCAAGAACTTGAGGCCATTGTTGGCTGCATACG GGGTCACCGCCTACCTGTGTGGGCATGACCACAACCTGCAGTATCTTCAGGATGAGAACGGTGTGGGCTA TGTGCTGAGTGGGGCCGGCAACTTCATGGACCCTTCCGTGCGGCATCAACGAAAGGTCCCCAACGGCTAC CTACGCTTTCACTATGGATCTGAGGACTCTCTGGGTGGCTTCACATACGTGGAGATAGGCTCCAAAGAAA TGAGCATCACGTATGTGGAAGCCTCTGGAAAATCACTCTTCAAGACCAGCCTCCCCAGGCGACCCAGACC CTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001270889
Insert Size	984 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001270889.1, NP_001257818.1
RefSeq Size	1473 bp
RefSeq ORF	984 bp
Locus ID	25732
UniProt ID	P29288
Gene Summary	tartrate-resistant acid phosphatase; may be involved in bone resorption; 5b isoform is osteoclast-specific and may be a specific marker of bone resorption [RGD, Feb 2006] Transcript Variant: This variant (2) contains an alternate 5' exon, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.