Panx1 (NM_001270549) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	px1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN216309 representing NM_001270549 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCATCGCCCACCTGGCCACGGAGTACGTGTTCTCGGACTTCTTGCTGAAGGAGCCCACCGAGCCCA AGTTCAAGGGGCTGCGACTGGAGCTGGCAGTGGACAAGATGGTCACGTGCATTGCCGTGGGTCTGCCTCT GCTGCTCATCTCGCTGGCCTTCGCTCAGGAGATCTCCATCGGTACCCAGATAAGCTGCTTCTCCCCAAGT TCCTTCTCCTGGCGCCAGGCCGCCTTCGTGGACTCTTACTGCTGGGCCGCTGTACAGCAGAAAAACTCCC TGCAGAGTGAGTCTGGAAACCTCCCGCTGTGGCTGCACAAGTTCTTCCCCTACATCCTGCTACTGTTTGC CATACTCCTGTACCTGCCCGCTCTGTTCTGGCGCTTTGCGGCGGCTCCACACCTCTGCTCCGACCTGAAG TTTATCATGGAGGAGCTTGACAAAGTCTATAACCGCGCCATCAAGGCTGCCAAGAGTGCTCGTGATTTGG ACCTGAGAGATGGACCTGGACCTCCAGGAGTGACTGAGAATGTGGGGCAGAGTCTGTGGGAGATATCCGA AAGCCACTTCAAGTACCCGATCGTGGAGCAGTACTTGAAGACGAAGAAGAACTCCAGTCACCTAATCATG AAATACATTAGCTGCCGGCTGGTGACCTTTGCGGTGGTACTGCTGGCTTGCATCTACTTGAGCTACTACT TCAGCCTCTCCTCGCTCTCCGACGAGTTTCTGTGTAGCATCAAGTCGGGCGTCCTGAGGAACGACAGCAC CATCCCCGATAGCTTCCAGTGCAAGCTCATCGCGGTCGGCATCTTCCAGCTGCTCAGCCTCATTAACCTC CTAGTGTACGCCCTGCTGGTCCCCGTGGTCATCTACACGCTCTTCGTCCCGTTCCGGCAGAAGACGGACG TCCTCAAGATTTGGACCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001270549
Insert Size	930 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001270549.1, NP_001257478.1
RefSeq Size	2173 bp
RefSeq ORF	930 bp
Locus ID	315435
Gene Summary	The protein encoded by this gene is a plasma membrane protein that is a structural component of gap junctions. The encoded protein acts as a homodimer or as a heterodimer with other isoforms or proteins. Two additional variants have been found, and the isoforms expressed from them are found in the cytoplasm. It is thought that these two isoforms could attenuate the actions of the membrane-bound protein. [provided by RefSeq, Jul 2012] Transcript Variant: This variant (4) uses an alternate splice junction at the 3' end of an exon compared to variant 1, that causes a frameshift. The resulting isoform (d) has a shorter and distinct C-terminus compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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