Cebpb (NM_001301715) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Il6dbp; NF-IL6; TCF5
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN216223 representing NM_001301715 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGTGGCCAACTTCTACTACGAGCCCGACTGCCTGGCCTACGGGGCCAAGGCGGCCCGCGCCGCGC CGCGCGCCCCCGCCGCCGAGCCGGCCATCGGCGAGCACGAGCGCGCCATCGACTTCAGCCCCTACCTGGA GCCGCTCGCGCCCGCCGCCGCGGACTTCGCCGCGCCCGCGCCCGCGCACCACGACTTCCTTTCCGACCTC TTCGCCGACGACTACGGCGCCAAGCCGAGCAAGAAGCCGTCCGACTACGGTTACGTGAGCCTCGGCCGCG CGGGCGCCAAGGCCGCACCGCCCGCCTGCTTCCCGCCGCCGCCTCCCGCCGCACTCAAGGCCGAGCCGGG CTTCGAACCCGCGGACTGCAAGCGCGCGGACGACGCGCCCGCCATGGCGGCCGGCTTCCCGTTCGCCCTG CGCGCCTACCTGGGCTACCAGGCGACGCCGAGCGGCAGCAGCGGCAGCCTGTCCACGTCGTCGTCGTCCA GCCCGCCCGGGACGCCGAGCCCCGCCGACGCCAAGGCCGCGCCCGCCGCCTGCTTCGCGGGGCCGCCGGC CGCGCCCGCCAAGGCCAAGGCCAAGAAGGCGGTGGACAAGCTGAGCGACGAGTACAAGATGCGGCGCGAG CGCAACAACATCGCGGTGCGCAAGAGCCGCGACAAGGCCAAGATGCGCAACCTGGAGACGCAGCACAAGG TGCTGGAGCTGACGGCGGAGAACGAGCGGCTGCAGAAGAAGGTGGAGCAGCTGTCGCGAGAGCTCAGCAC GCTGCGGAACTTGTTCAAGCAGCTGCCCGAGCCGCTGCTGGCCTCGGCGGGTCACTGCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001301715
Insert Size	831 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001301715.1, NP_001288644.1
RefSeq Size	1475 bp
RefSeq ORF	831 bp
Locus ID	24253
UniProt ID	P21272
Gene Summary	This intronless gene encodes a member of the transcription factor family whose members contain a basic leucine-zipper domain. The encoded protein functions as a homodimer but can also form heterodimers with CCAAT/enhancer-binding proteins alpha, delta, and gamma. The encoded protein plays important roles in several cellular processes and in various diseases, including regulating cell proliferation, differentiation, apoptosis and neuroinflammation, and being involved in brain injury and inflammatory progression. The use of alternative in-frame AUG start codons results in multiple protein isoforms, each with different cellular localizations and distinct biological functions. [provided by RefSeq, Sep 2014] Transcript Variant: This variant (1) encodes multiple isoforms through the use of alternative translation initiation codons. The isoform [LAP2, also known as LAP (liver activating protein), p34 or CRP2 (c/EBP-related protein 2)] represented in this RefSeq results from translation initiation at a downstream AUG start codon. Isoform LAP2 has a shorter N-terminus, compared to isoform LAP1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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