Gnas (NM_001159653) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	ALEX; G-alpha-8; Gnas1; Gnpas; Nesp55; SCG6
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN216170 representing NM_001159653 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCGCAGGTCCCGGGCTCATCAGTGGCGCCGAGCTCGCCATAATTACAACGACCTGTGCCCGCCCA TAGGCCGCCGGGCTGCTACCGCTCTCCTCTGGCTCTCCTGCTCCATCGCTCTCCTCCGCGCCCTAGCCTC TTCCAACGCCCGCGCCCAGCAGCGGGCTGCCCAGCGCCGGAGCTTCCTTAACGCCCACCACCGCTCCGCT GCCGCTGCAGCTGCCGCACAGGTACTCCCCGAGTCCTCTGAATCCGAATCTGATCACGAGCACGAGGAGG CTGAGCCTGAGCTGGCCCGCCCCGAGTGCCTAGAGTACGATCAGGACGACTACGAGACCGAGACCGATTC TGAGACCGAGCCTGAGTCCGATATCCAGTCCGAGACCGAATTCGAGACCGAGCCTGAGACCGAGCCTGAG ACCGCCCCTACAACTGAGCCTGAGACCGAACCAGAGGACGAGCGCGGCCCCCGGGGCGCCACCTTCAACC AGTCACTCACTCAGCGTCTGCACGCTCTGAAGTTGCAGAGCGCCGACGCCTCCCCGAGACGTGCGCAGCC CACCACTCAGGAGCCTGAGAGCGCAAGCGAGGGGGAGGAGCCCCAGCGCGAGCCCTTAGACGAGGATCCT CGGGACCCCGAGGAGTCAGAGGAGCTCAGGGAGGCGAACAGGCAGCCCCGCCGCTGCAAGACCAGGAGGC CAGCCCGCCGTCGCGACCAGTCCCCGGAGTCCCCTCCCAGAAAGGGGCCCATCCCCATCCGGCGTCACTA A ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001159653
Insert Size	771 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001159653.1, NP_001153125.1
RefSeq Size	2543 bp
RefSeq ORF	771 bp
Locus ID	24896
UniProt ID	Q792G6
Gene Summary	This locus has a highly complex imprinted expression pattern. It gives rise to maternally, paternally, and biallelically expressed transcripts that are derived from four alternative promoters and 5' exons. Some transcripts contain a differentially methylated region (DMR) at their 5' exons, and this DMR is commonly found in imprinted genes and correlates with transcript expression. In addition, one of the transcripts contains a second overlapping ORF, which encodes a structurally unrelated protein - Alex. Alternative splicing of downstream exons is also observed, which results in different forms of the stimulatory G-protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants have been found for this gene. [provided by RefSeq, Apr 2009] Transcript Variant: This variant (4) is maternally expressed. It has alternate 5' exons, as compared to variant 3. Variants 4 and 5 both encode neuroendocrine secretory protein 55 (NESP55), which localizes to large secretory vesicles of endocrine cells and neurons. The coding regions of variants 4 and 5 do not overlap the coding regions used by other transcripts; thus NESP55 has no similarity to isoforms of the G-protein alpha subunit.

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