Ngf (NM_001277055) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	beta-NGF; Ngfb
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN216118 representing NM_001277055 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCATGTTGTTCTACACTCTGATCACAGCGTTTTTGATCGGCGTACAGGCAGAACCGTACACAGATA GCAATGTCCCAGAGGGAGACTCTGTCCCTGAAGCCCACTGGACTAAACTTCAGCATTCCCTTGACACAGC CCTCCGCAGAGCCCGCAGTGCCCCTGCTGAACCAATAGCTGCCCGTGTGACAGGGCAGACCCGCAACATC ACTGTGGACCCCAAACTGTTTAAGAAACGGAGACTCCGTTCACCCCGCGTGCTGTTTAGCACCCAGCCTC CACCCACCTCTTCGGACACTCTGGATTTAGACTTCCAGGCCCATGGTACAATCTCCTTCAACAGGACTCA CAGGAGCAAGCGCTCATCCACCCACCCAGTCTTCCACATGGGGGAGTTTTCAGTGTGTGACAGTGTCAGT GTGTGGGTTGGAGATAAGACCACAGCCACGGACATCAAGGGCAAGGAGGTGACAGTGCTGGGCGAGGTGA ACATTAACAACAGTGTATTCAAACAGTATTTTTTTGAGACCAAGTGCCGAGCCCCGAATCCTGTAGAGAG TGGATGCCGGGGCATTGACTCCAAGCACTGGAACTCATACTGCACCACGACTCACACCTTTGTCAAGGCG TTGACAACAGACGACAAACAGGCTGCCTGGAGGTTCATCAGGATAGATACAGCCTGCGTGTGTGTGCTCA GCAGGAAGGCTGCAAGAAGAGGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001277055
Insert Size	726 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001277055.1, NP_001263984.1
RefSeq Size	1075 bp
RefSeq ORF	726 bp
Locus ID	310738
UniProt ID	P25427
Gene Summary	Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades to regulate neuronal proliferation, differentiation and survival (By similarity). The immature NGF precursor (proNGF) functions as ligand for the heterodimeric receptor formed by SORCS2 and NGFR, and activates cellular signaling cascades that lead to inactivation of RAC1 and/or RAC2, reorganization of the actin cytoskeleton and neuronal growth cone collapse. In contrast to mature NGF, the precursor form (proNGF) promotes neuronal apoptosis (in vitro) (By similarity). Inhibits metalloproteinase-dependent proteolysis of platelet glycoprotein VI (By similarity). Binds lysophosphatidylinositol and lysophosphatidylserine between the two chains of the homodimer. The lipid-bound form promotes histamine relase from mast cells, contrary to the lipid-free form (By similarity).[UniProtKB/Swiss-Prot Function]

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