Pcp4 (NM_001270538) Rat Untagged Clone

CAT#: RN215788

Pcp4 (untagged) - Rat Purkinje cell protein 4 (Pcp4), transcript variant 2



  "NM_001270538" in other vectors (1)

CNY 2,000.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Synonyms pep-19; PEPZ19
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN215788 representing NM_001270538
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAAAGACAAGACATCAGGAGATAATGGCAAAGTGCTCACTGCCAGAGGAGGAATGGAGAAGTGCCT
GGCACGATCCAGCCAGAACCTGCTCGGTCTCTAAGAAAGCCAAGAGGATCTGCAGTGGAAATGCCTCGCT
GGAGGCTCCTGAAATATATGGGCAGAAGAAGGTCCAAGAAGAATTTGATATCGACATGGATGCACCAGAG
ACAGAGCGTGCAGCTGTGGCCATTCAGTCTCAGTTCAGAAAATTCCAGAAGAAAAAGGCAGGATCACAGT
CCTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001270538
Insert Size 285 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001270538.2, NP_001257467.1
RefSeq Size 698 bp
RefSeq ORF 285 bp
Locus ID 25510
Gene Summary The protein encoded by this gene regulates H1˚ and H3.3 histone synthesis by binding to H1˚ and H3.3 histone mRNAs. The encoded protein can bind calmodulin as well, providung a possible link between calcium-dependent signals and histone metabolism. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2012]
Transcript Variant: This variant (2) contains an alternate exon compared to variant 1, which causes a frameshift and use of a different start codon compared to variant 1. The resulting isoform (LPI) has a longer and distinct N-terminus compared to isoform PEP19. Experimental support for this transcript and isoform is provided in PMID:22020791 and PMID:17273800.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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