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Plcz1 (NM_001012234) Rat Untagged Clone
CAT＃: RN215649
Plcz1 (untagged ORF) - Rat phospholipase C, zeta 1 (Plcz1), (10 ug)
CNY 8,360.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN215649 representing NM_001012234
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGAGCCATTATGAGCTCGCAGAAGCAAGATGGTTTATGTCAAAGATTCAGGATTATTTTAGAGGTG GAAAAATCAGCGCTGGAATTACTCACAAACTGCTCGAGAAACTTGATTTCCCATGCCATTTTGCTCATGT GAAACGTATTTTTAAGGAAAATGACAGACATAACCAAGGAAGAATCACCACCGAAGATTTTAGAACCATC TATCGGTGTATTGTACATAGAGAAGAGATCGTTGAGATTTTCAACACGTATACTGAAAACAGGAAAATTC TCCCCGAGGACAGTCTGATTGAATTTCTAACCCAAGAGCAGTATGAAATGGAGATGGATGAGTCCAGTTC AGTGGAGATCATCCAGAAGTACGAGCCCATTGCAGAAGTAAAGAACGAGCGGCAGATGTCAATTGAAGGT TTTGCAAGATACATGTTTTCTTCAGAATGTCTACTGTTTAAAGAGACGTGTAACACAGTGTACCAAGATA TGAATAAGCCACTAAATGATTACTATATTTCATCGTCTCACAACACATATTTGATATCTGATCAAATATT GGGACCAAGTGACATTTGGGGATATATAAGTGCCCTCGTGAAAGGCTGCCGCTGTCTGGAAATTGACTGC TGGGATGGAGCACAAAATGAACCCATTGTGTACCATGGCTACACTCTCACCAGCAAGCTTCTCTTCAAAA CCGTTATCCAAGCAATAAACAAGTACGCCTTCGTGACGTCTGATTACCCAGTGGTGCTGTCCTTAGAGAA TCACTGCTCCCCTGGTCAACAGGAAGTGATGACCGACATTCTGCAGAGTACCTTTGGAGACTTTCTGCTC TCAGACATACTTGACGAGTTTCCAGACAGTTTGCCATCTCCAGAGGCACTAAAATTCAAAATATTAGTGA AAAATAAGAAAGTTGGAACCTTATCTGAAACCCGCGAGAGGCTGGGGACTGACAAAAGGGGCATAGCGCT AGACTTGGAAGAAGAAATCTATGAAAATGAAGACGAAGACTCAGGAAAGGAGCCAGAAACGTGGGATGAT TTCCTGTCACGGGTTAAGGAGGAGCAGGAGGCAGACCCCTCAACGTTGAGCGGAATAGCAGATGCCAAGA AAAAGATCAGGAAGCTAAGAGTAGCTCTGGCCTTATCTGATCTTGTCATTTATACCAAAGCTGAGAAGTT CCGAAACTTCCAATATTCAAGAGTCTATCAGCAGTTTAATGAGACCACTTCTATGGGAGAGTCTCGAGCT CGAAAACTTTCAAAATTGAGAGCCCATGAGTTTATTTTCCACACTGCAGCGTTCATCACCAGAGTGTACC CCAAGTTCACGAGAGCAGACTCTTCTAATTTTAATCCTCAAGAGTTTTGGAATGTGGGCTGTCAGATGGT GGCCTTGAATTTTCAAACCCCTGGACTGCCTATGGATTTGCAAAACGGGAAATTTTTGGATAATGGAGGC TCTGGATATGTTTTGAAGCCAGACTTTCTTAGAGACACAACTTTGGGCTTTAACCCAAATGAACCAGAAG GAGATGGCCATCCGGTTACCCTCACGATCCGACTCATCAGTGGGATCCAGTTGCCTGTTAACGTGCCCTC AAATACATCTGACATAATAGTGATCATAGAAGTCTACGGTGTCCCAAACGACCACATGAAGCAGCAGAGT CGTGCCGTTAAGAACAATGCTTTTAGTCCAAGGTGGAATGAAACATTCACATTTCTTATTCAAGTGCCAG AATTGGCACTGATACGTTTCGTTGTTGAAACTCAAGGCTTCCTATCGGGAAATGAATTACTTGGGCAGTA CACTTTACCCGTTCTTTGCATGAACAAAGGTTATCGTCGCGTTCCTCTGTTTTCCAAATCCGGTGCGAAC CTTGAACCTTCCTCTCTGTTTATTTACGTTTGGTACTACAGAGAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001012234 |
| Insert Size | 1938 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001012234.1, NP_001012234.1 |
| RefSeq Size | 1938 bp |
| RefSeq ORF | 1938 bp |
| Locus ID | 497197 |
| UniProt ID | Q5FX52 |
| Gene Summary | The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. In vitro, hydrolyzes PtdIns(4,5)P2 in a Ca(2+)-dependent manner. Triggers intracellular Ca(2+) oscillations in oocytes solely during M phase and is involved in inducing oocyte activation and initiating embryonic development up to the blastocyst stage. Is therefore a strong candidate for the egg-activating soluble sperm factor that is transferred from the sperm into the egg cytoplasm following gamete membrane fusion. May exert an inhibitory effect on phospholipase-C-coupled processes that depend on calcium ions and protein kinase C, including CFTR trafficking and function.[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR215649 | Plcz1 (Myc-DDK-tagged ORF) - Rat phospholipase C, zeta 1 (Plcz1), (10 ug) |
CNY 8,360.00 |
|
| RR215649L3 | Lenti ORF clone of Plcz1 (Myc-DDK-tagged ORF) - Rat phospholipase C, zeta 1 (Plcz1), (10 ug) |
CNY 10,170.00 |
|
| RR215649L4 | Lenti ORF clone of Plcz1 (mGFP-tagged ORF) - Rat phospholipase C, zeta 1 (Plcz1), (10 ug) |
CNY 11,120.00 |
