Ogdh (NM_001017461) Rat Untagged Clone
CAT#: RN215419
Ogdh (untagged ORF) - Rat oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide) (Ogdh), nuclear gene encoding mitochondrial protein, (10 ug)
CNY 16,440.00
Product images
Specifications
Product Data | |
Type | Rat Untagged Clone |
Tag | Tag Free |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RN215419 representing NM_001017461
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTCATTTAAGGACTTGTGCTGCTAAGTTAAGGCCATTGACAGCCTCCCAGACTGTTAAGACATTTT CACAAAACAAACCAGCAGCAATTAGGACGTTTCAACAGATTCGGTGCTATTCTGCACCTGTAGCTGCTGA ACCATTTCTTAGTGGGACTAGTTCGAACTATGTGGAGGAAATGTACTGTGCCTGGTTGGAGAATCCCAAA AGTGTACATAAGTCATGGGACATTTTTTTCCGAAACACCAATGCTGGAGCCCCACCGGGCACTGCCTACC AGAGCCCCCTTTCCCTGAGTCGAAGCTCCCTGGCTACCATGGCTCATGCACAGTCCCTGGTGGAAGCACA GCCTAACGTCGACAAGCTCGTGGAGGACCACTTGGCGGTGCAGTCTCTCATCAGGGCGTATCAGATACGA GGGCACCATGTAGCACAGCTGGACCCCCTGGGGATTTTGGATGCTGATCTGGACTCCTCCGTGCCCGCTG ACATTATCTCATCCACAGACAAACTTGGGTTCTATGGCCTACACGAGTCTGACCTTGACAAGGTCTTCCA CTTACCCACCACCACTTTCATCGGGGGACAGGAGCCAGCACTTCCTCTTCGGGAGATCATCCGTCGGCTG GAGATGGCCTACTGCCAGCACATTGGTGTGGAGTTCATGTTCATTAACGATTTGGAGCAATGCCAGTGGA TCCGGCAGAAGTTTGAGACCCCTGGGATCATGCAGTTCACCAATGAGGAGAAGCGGACCCTGCTGGCCAG GCTTGTACGGTCCACCAGGTTTGAGGAGTTCCTGCAGCGAAAGTGGTCCTCTGAGAAGCGTTTTGGTCTG GAAGGCTGTGAGGTGCTGATCCCTGCCCTCAAGACAATCATTGACATGTCAAGTGCAAATGGAGTGGACT ATGTGATCATGGGGATGCCACACAGAGGACGACTGAACGTGCTAGCCAATGTCATCAGGAAGGAGCTGGA GCAGATATTCTGTCAGTTTGACTCGAAGCTGGAGGCAGCTGATGAGGGTTCCGGGGACATGAAGTACCAC CTGGGCATGTATCACCGAAGGATCAACCGTGTGACCGACAGAAACATCACTCTGTCCTTGGTGGCTAACC CTTCCCACTTAGAGGCTGCTGACCCTGTAGTGATGGGAAAGACCAAAGCTGAACAGTTCTACTGTGGAGA CACTGAAGGGAAAAAGGTGATGTCTATCCTGCTGCATGGGGATGCTGCCTTTGCTGGCCAGGGTATCGTG TATGAGACCTTCCATCTCAGCGACCTGCCATCCTATACAACCCACGGCACCGTTCACGTGGTTGTCAACA ACCAGATTGGCTTCACCACAGACCCTCGGATGGCCCGCTCTTCTCCCTACCCCACTGATGTGGCCCGAGT GGTGAACGCCCCCATTTTCCATGTCAACTCAGATGACCCTGAAGCTGTCATGTATGTATGCAAGGTGGCA GCTGAGTGGAGAAACACCTTCCACAAGGATGTTGTTGTTGATCTGGTGTGTTATCGACGAAATGGCCACA ACGAGATGGATGAACCTATGTTCACACAGCCACTCATGTACAAGCAGATCCGTAAGCAGAAGCCGGTACT GCAGAAGTATGCAGAATTGCTGGTATCCCAGGGGGTCGTCAACCAGCCCGAATATGAGGAGGAAATCTCC AAGTACGATAAGATCTGTGAGGAAGCATTTACCAGATCCAAAGATGAGAAGATCTTGCACATCAAGCACT GGCTGGATTCCCCCTGGCCTGGCTTTTTCACCCTGGATGGACAGCCCAGGAGCATGACCTGCCCCTCCAC TGGCCTGGAGGAGGACATCTTGACCCACATTGGGAATGTGGCCAGTTCTGTACCTGTGGAGAACTTTACC ATCCATGGAGGGCTGAGCCGGATCTTGAAGACTCGCAGAGAGCTTGTGACAAACCGGACTGTGGACTGGG CCCTGGCAGAGTACATGGCGTTCGGATCACTCCTGAAGGAAGGCATCCATGTCCGGCTGAGTGGCCAGGA CGTGGAGCGGGGTACCTTCAGCCATCGCCACCATGTGCTCCATGACCAGAATGTGGACAAAAGAACCTGC ATCCCCATGAACCACCTTTGGCCTAACCAGGCCCCTTACACCGTGTGCAACAGCTCGTTGTCTGAGTATG GTGTCCTGGGCTTTGAACTGGGCTTTGCCATGGCTAGCCCTAATGCTCTGGTTCTCTGGGAGGCCCAGTT TGGTGATTTCAACAACATGGCACAGTGCATCATTGACCAGTTCATCTGCCCAGGACAGGCAAAGTGGGTG CGGCAGAATGGCATCGTGCTCCTGCTACCTCACGGCATGGAAGGCATGGGTCCCGAGCATTCCTCTGCCC GTCCAGAGAGGTTTCTGCAGATGTGCAATGATGACCCAGATGTCCTGCCTAACCTGCAGGAGGAAAACTT TGACATCAGTCAGCTCTACGACTGCAACTGGATTGTCGTCAACTGCTCCACCCCTGGCAACTTCTTCCAC GTGCTGCGACGACAGATCTTGCTGCCCTTCCGGAAGCCGTTAATCGTCTTCACTCCCAAATCCCTCCTGC GCCACCCTGAGGCAAGAACTAGCTTTGATGAGATGCTGCCAGGAACCCACTTCCAGCGTGTGATCCCAGA AGATGGACCTGCAGCTCAGAACCCAGACAAAGTCAAGAGGCTTCTCTTCTGCACTGGCAAGGTGTACTAT GACCTCACCCGAGAGCGCAAAGCCAGGGACATGGCAGAGGAGGTGGCTATTACAAGGATTGAGCAGCTGT CACCATTCCCCTTCGACCTCCTGCTGAAAGAGGCACAGAAGTATCCCAATGCTGAGCTGGCCTGGTGCCA GGAAGAGCACAAGAATCAAGGCTACTATGACTATGTCAAGCCAAGACTTCGGACCACCATTGACCGTGCT AAGCCTGTCTGGTATGCTGGCCGAGACCCAGCAGCCGCTCCAGCCACTGGCAACAAGAAGACCCACCTGA CAGAGCTGCAGCGCTTTCTGGACACAGCCTTTGACCTGGACGCCTTCAAGAAATTCTCTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_001017461 |
Insert Size | 3072 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001017461.1, NP_001017461.1 |
RefSeq Size | 3266 bp |
RefSeq ORF | 3072 bp |
Locus ID | 360975 |
UniProt ID | Q5XI78 |
Gene Summary | 2-oxoglutarate dehydrogenase (E1) component of the 2-oxoglutarate dehydrogenase complex, which mediates the decarboxylation of alpha-ketoglutarate. The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). The 2-oxoglutarate dehydrogenase complex is mainly active in the mitochondrion. A fraction of the 2-oxoglutarate dehydrogenase complex also localizes in the nucleus and is required for lysine succinylation of histones: associates with KAT2A on chromatin and provides succinyl-CoA to histone succinyltransferase KAT2A.[UniProtKB/Swiss-Prot Function] |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RR215419 | Ogdh (Myc-DDK-tagged ORF) - Rat oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide) (Ogdh), nuclear gene encoding mitochondrial protein, (10 ug) |
CNY 16,440.00 |
|
RR215419L3 | Lenti ORF clone of Ogdh (Myc-DDK-tagged ORF) - Rat oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide) (Ogdh), nuclear gene encoding mitochondrial protein, (10 ug) |
CNY 18,050.00 |
|
RR215419L4 | Lenti ORF clone of Ogdh (mGFP-tagged ORF) - Rat oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide) (Ogdh), nuclear gene encoding mitochondrial protein, (10 ug) |
CNY 19,860.00 |