Rec8 (NM_001011916) Rat Untagged Clone
CAT#: RN215104
Rec8 (untagged ORF) - Rat REC8 homolog (yeast) (Rec8), (10 ug)
CNY 7,510.00
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Specifications
Product Data | |
Type | Rat Untagged Clone |
Tag | Tag Free |
Synonyms | Rec8L1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RN215104 representing NM_001011916
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCTACTATCCCAACGTGCTTCAGCGCCACACAGGCTGCTTCGCCACTATCTGGCTGGCCGCTACCC GGGGCAGCCGTTTGGTGAAGCGGGAATACCTGAAAGTGAACGTGGTAAAGACCTGCGAGGAAATCCTCAA TTATGTGCTCGTACGAGTGCAACCTCCGATGCCTGGGCTGCCCCGCCCCCGCTTCTCCCTCTATCTCTCC GCCCAGCTTCAGATTGGTGTGATAAGGGTCTACTTTCAACAGTGCCAGTACCTTGTGGAAGACATCCAGC ATATCCTAGAGCACCTACACCGGGCCCAGCTGCGGATTCGCATTGATATGGAGGAGGCTGACTTACCTAG CTTGCTTCTTCCCAATTGCCTGGCCATGATGGAGACGCTGGAGGACGCCCCAGAACCCTTTTTTGGGACG ATGTCTGTGGACCCTACACTTCCCAGTCCTTTTGATATTCCTCAGATTCGACACCTTTTAGAGGCTGCAA CCCCGGAGAAAATTCGTGAGGAGACCCTTCCTGAAGCCACGCCGGAGCCCAGGAAGCCTGACAGGACCTT GGCCATGGTACAGTCCCCTGAGGTCATCACCTTGCAGGAGGCAGAGCCCATCCGAATGCTGCGGATTGAG GGAGAGCAGGATCTCCCAGAGATCAGCAGAGGAGACTTAGACCTGCTGATCGCAGAGGAAGATGACGCCA TATTGCTGGAGGAACGTCAGAGAGGCAGACTTCTCCGGCAGCGGAGGGCTTCCCCACCACTGGATGAATC CAAGGAAGAGCCCCGGGCCCTGGAAGGAGATGGTTTGGTCTCCTCACTCTCACCTCCAGCTCCTGCACAG GTTGAAGGGATAAGAGAACCACTTCCAGACCAGGCCTTTCCCCCTGAGGTACAGAAGATGCCAGGCTGGG AGCCTGGGGTCCTACTCACTGAGGTGACCCCACCTCAGGAGCTGCGTCTGCCTGCCCCACCGAGTATAGA GAAGAGGCCCCCGTCTCCTCAGAGGCCACCTCGCCGGCGCCGCCGGGGGCGCCAGTTATTGTTCTGGGAC AAGGAGACCCAGATCTCCCGGGAGAAGTTTGAGGAACAGCTGCAGACTGGGGCTCACTGCTGCGAGTATC CAGTGGTCCAGCCTCCCAAGAGGAAGCTCATGAGCCCAGCGGAGCTCTTCAGAACCCCAACTCTCTCTGG CTGGTTATCTCCAGAACTACTAGCTTTATGGACCCATTGTGCCCAGGTTCCCCCAAGAATGCTCAGACAA AGGCCACAGCTGGAGACTGAAGAGACAGTTGAGGAAGAGAGAGTGGCAGACAAGGAAGAAAGGAGAAAGA CTGAAGCTCTGAGTGAGATTGAGGTTCTGAGAGAGGCCCAGGAGCCCAGTGGTCCCCTCATGTTGTCTTC AGAGCTCTCCCTAGAAGTAGCAGAAGAAGAAAAATCTCGAACTAGCTTCATCCCTCCAGAAGAACGGTGG GCCTGGATTGAGGAGAGGCAGCCAGAGCCCCCTGCACTGCCCATGCTGCCTGAACTTCCTGAAGTGCCCA TGGAGATGCCCCCAGGGCCTGAACTACTCTCTTCAGAGGCTGTGCTTAGGGCAGTGGCTCTGGAGCTGCA GGCTAACAGGGAGCCTGACTTCAGCAGCCTGGTGCCCCCTCTCAGCTCCCGAAAGTTGGCTTCTCGGGTC TTCTATCTGCTCTTGGTGCTGTCCGCACAGAAGATCCTTCTTGTGGACCAACAGAAGCCATATGGGCGCC TCCTGATTCGTCTAGGACCCAAATTCCCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_001011916 |
Insert Size | 1782 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001011916.1, NP_001011916.1 |
RefSeq Size | 2049 bp |
RefSeq ORF | 1782 bp |
Locus ID | 290227 |
UniProt ID | Q6AYJ4 |
Gene Summary | Required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II (By similarity).[UniProtKB/Swiss-Prot Function] |
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RR215104 | Rec8 (Myc-DDK-tagged ORF) - Rat REC8 homolog (yeast) (Rec8), (10 ug) |
CNY 7,510.00 |
|
RR215104L3 | Lenti ORF clone of Rec8 (Myc-DDK-tagged ORF) - Rat REC8 homolog (yeast) (Rec8), (10 ug) |
CNY 9,500.00 |
|
RR215104L4 | Lenti ORF clone of Rec8 (mGFP-tagged ORF) - Rat REC8 homolog (yeast) (Rec8), (10 ug) |
CNY 10,360.00 |