Plce1 (NM_053758) Rat Untagged Clone

CAT#: RN214972

Plce1 (untagged ORF) - Rat phospholipase C, epsilon 1 (Plce1), (10 ug)



  "NM_053758" in other vectors (1)

CNY 43,890.00


货期*
12周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Synonyms Plce
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN214972 representing NM_053758
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACGTCCGAAGAAATGGCCGCTTCCTTCCTCATCCCGGTGCCTCAAAGGAAAGTGGCTTCTGCCCAGT
CAGTGGCAGAGGAGCGGGGTGAAAAGGTCTCAGAAGCCGGCATTCCTAAGACACGAGCTGGCAGACAGGG
TGGGCTCACCCCTCGTACCATCTCACAGCGGAACGAACCTGAGGAAGAGTCTCCTAGAACTGATTTTTCC
CAGGTCTTCTCAATAGCGAGGGGGGAACTAGACAGCGACGAGAATCACAATGAAAGGTGCTGGGAGGAAA
ATGTGCCAGGTTCCACGAAAAACCACGCGGTTAACTGCAACAGCTTATTGCAAAGCCACCAGCATGCGCT
TCCCCCGAGCCAGCTCTGTGAAGTCTGTGACTCTGTCACAGAAGAACACCTGTGTTTGCAGCCTGGGATT
CCTTCTCCACTGGAAAGGAAGGTGTTTCCTGGGATCGAACTGGAGATGGAGGACTCTCCCATGGACGTGA
GCCCTTTGGGAAACCAGCCTGGGATCATGGAGTCCAGCGGACCTCACTCTGACCGCAACATGGCGGTATT
TCATTTCCATTATGCAGGTGACAGGACAATGCCAGGTGCTTTCCATACCCTGTCAGAAAAATTCATTTTG
GATGACTGTGCAAACTGTGTTACTCTTCCTGGGGGGCAGCAAAACAAAAATTACATGGCGTATACTTGCA
AACTGGTGGAGTTGACAAGAACCTGTGGTAGTAAGAATGGGCAGCTGAAGTGTGACCACTGCACCTCGCT
GCGGGATGAATACCTGTGCTTTGAAAGCTCCTGCCGGAAGGCTGAGGCTCTCTCCTCAGGTGGTGGCTTT
TGCGAGGACGGATTTACTCATGGTCCCTCTGCCAAGACTTTTCTGAACCCTTTGGAGGAATTCTCTGATA
ATTGTGAAGACGTGGACGATATTTTTAAAGGCAAAAAGGAGCGGTCCACTTTGTTAGTCCGAAGGTTTTG
TAAAAATGACAGGGAAGTTAAGAAGTCTGTGTATACTGGGACAAGGGCCATTGTGAGAACCCTGCCTTCT
GGCCACATTGGGCTGGCTGCTTGGAGTTACGTCGATCAGAAGAAAGCTGGTCTCATGTGGCCTTGTGGGA
ATGGAATGAGACCTCTGTCCACAGTGGACGTAAGGCAAAGTGGGAGACAGCGTCTGTCTGAAGCCCAGTG
GTGTCTGATCTACAGTGCCGTGAGAAGAGAAGAGACAGAAGATACGGTTGGCTCTCTTCTGCATTGCTCC
ACACAGCTCCCAACCCCTGACACAGCCCATGGAAGGATCGGAGATGGTCCCTGCCTAAAGCAATGTGTGC
GAGACAGCGAGTGTGAGTACCGTGCCACCCTCCAAAGGACGTCCATAGCTCAGTACATCACCGGTTCTCT
CCTAGAGGCAACCACATCTTTAGGAGCAAGAAGCAGCCTTCTCAGTTCCTTTGGAGGATCCACTGGGCGA
ATAATGCTGAAAGAACGCCAACCGGGCACTTCTATGGCCAATTCCAGTCCTGTTCCTTCAAGTTCAGCTG
GAATCAGCAAGGAGCTGATTGATCTCCAGCCCCTCATTCAGTTCCCGGAGGAAGTAGCCAGCATCTTAAC
AGAACAGGAACAGAACATTTACCGCAGGGTCCTGCCCATGGACTACCTCTGTTTCTTAACCCGGGACTTG
AGTTCCCCCGAGTGTCAGAGATCACTGCCCCGTCTCAAAGCATGCATCTCAGAATCAATCCTCATGTCTC
AAAGTGGAGAGCACAATGCCCTTGAAGACCTCGTGATGAGATTTAACGAGGTGAGCTCCTGGGTAACATG
GCTGATCCTCACGGCAGGCTCCATGGAGGAGAAGCGGGAAGTCTTTTCTTATTTGGTGCATGTGGCCAAA
TGCTGCTGGAACATGGGCAACTACAATGCTGTCATGGAGTTCCTGGCTGGCCTCAGGTCAAGGAAAGTTC
TGAAGATGTGGCAGTTCATGGACCAGTCTGACATCGAGACCATGAGGAGCCTGAAAGACGCCATGGCTCA
GCATGAGTCTTCCGTGGAGTACAAGAAGGTAGTGACCCGAGCCTTGCACATCCCCGGCTGCAAAGTGGTT
CCCTTCTGTGGGGTGTTCCTGAAGGAGCTCTGTGAGGTGCTTGATGGTGCCTCTGGCCTCCTGAAGCTCT
GCCCACGATACAGCTCCCAAGAAGAAGCCCTGGAGTTTGTAGCGGATTACAGTGGACAAGATAACTTCTT
ACAGCGAGTGGGGCAGAATGGCTTGAAGAATCCAGAGAAGGAGCTCACGGTCAACAGCATCTTTCAGATC
ATCCGGAGCTGCAGCCGAAGCTTGGAGACGGAGGACGAGGAGAGCGCCAGCGAAGGGAGCGGCTCTAGGA
AAAACTCCCTCAAGGACAAAACCCGGTGGCAGTTTATAATTGGAGATTTGCTGGATTCAGACAATGACAT
CTTTGAGAAGTCCAAAGAGTGCGACCCTCATGGATCAGAGGAGTCACAGAAAGCCTTTGACCATGGGACA
GAGCTCATCCCATGGTATGTGCTGTCCATCCAAGCGGATGTGCACCAGTTCCTCCTGCAGGGGGCCACAG
TCATCCACTATGACCAGGACACACATCTCTCCGCCCGCTGCTTTCTCCAGTTGCAGCCTGACAATAGTAC
CTTGACCTGGATGAAACCTCCCACTGCCTCCCCAGCTGGTGCCAGACTCAAGCTCGGTGTGCTTAGTAAC
GTGGCCGAACCTGGCAAGTTCCCATCACTGGGCAACGCTGGGGTGAGCGGCCTGGTAGAGGGAATCCTGG
ATCTGTTTTCAGTGAAGGCTGTGTACATGGGACACCCTGGCATTGATATACACACTGTGTGTGTCCAGAA
CAAACTTAGTAGCATGCTTCTGTCAGAGACTGGGGTGACGCTGCTCTATGGGCTTCAGACCACCGACAAT
AGATTACTGCACTTCGTGGCACCAAAGCACACAGCTAAAATGCTTTTCAGTGGATTGCTGGAACTCACTA
CAGCTGTGAGAAAGATACGGAAGTTCCCTGACCAAAGACAACAGTGGCTTCGGAAACAGTATGTCAGCTT
CTATCAGGAGGATGGTCGGTATGAAGGCCCAACCTTGGCACATGCTGTGGAGTTGTTTGGTGGTAGGAGA
TGGAGCACTAGAAACCCCAGCCCTGGGATGTCAGCAAAGAATGCCGAGAAGCCCAACATGCAGAGAAACA
ACACCCTAGGCATAAGCACCACCAAGAAAAAGAAGAAAATGCTCATGAGGGGGGAGAGTGGAGAGGTGAC
GGACGATGAGATGGCCACCCGCAAGGCCAAAATGTACAGAGAGTGTCGCAGCCGGAGCGGCTCTGATCCT
CAAGAGGCTAATGAACAGGAAGACTCAGAGGCCAATGTCATCACTAACCCTCCCAACCCCCTCCATTCCA
GAAGAGCCTACTCATTGACCACGGCTGGGTCCCCCAACTTGGCTACCGGGATGTCATCTCCTATCAGTGC
CTGGAGTAGCAGCAGCTGGCATGGGCGGATCAAAGGAGGAATGAAAGGCTTCCAGAGCTTCATGGTCTCA
GACAGTAACATGAGTTTCATTGAATTCGTCGAGCTGTTCAAATCATTCAGCATAAGGAGCCGCAAGGACT
TGAAGGACATCTTTGACATCTACTCTGTACCCTGTAATCGGTCTGCTTCTGAGTCAACCCCACTGTACAC
CAACCTGACAATCGAAGAAAATACCAATGACCTTCAGCCTGACCTAGATTTGCTGACTAGAAACGTCTCC
GACTTGGGGCTGTTCATGAAGAGTAAGCAGCAGCTTTCTGACAACCAGAGGCAGATATCTGACGCCATTG
CTGCTGCAAGCATTGTGACTAATGGCACTGGGATTGAAAGCACGTCCCTGGGCATATTTGGGGTCGGCAT
ACTCCAGCTCAACGACTTCCTGGTGAATTGCCAAGGAGAACACTGCACATATGATGAAATCCTCAGCATC
ATCCAGAAGTTCGAGCCCAACATCAGCATGTGTCATCAAGGTCTATTGTCCTTCGAAGGGTTTGCAAGGT
TTCTGATGGATAAAGACAATTTCGCCTCAAAAAATGACGAGTCGCGGGAGAACAAGAAAGACCTGCAGCT
ACCCCTCTCCTACTATTACATCGAATCTTCGCACAACACCTACCTCACCGGCCATCAGCTCAAGGGGGAG
TCCTCTGTGGAGCTCTACAGCCAGGTCCTCTTGCAAGGATGCAGAAGCATAGAGCTGGACTGCTGGGATG
GAGATGATGGGATGCCCATTATTTATCATGGACATACGCTGACAACCAAGATCCCCTTCAAGGAAGTGGT
GGAAGCCATCGATCGCAGTGCCTTCATCACCTCCGACCTGCCAATCATCATATCCATTGAGAACCATTGC
TCATTGCCTCAGCAACGGAAAATGGCAGAAATTTTCAAGAGTGTGTTTGGAGAAAAGCTGGTGGCTAAGT
TCTTGTTCGAGACCGATTTCTCAGATGACCCAATGCTTCCCTCACCTGACCAACTTAGAAGGAAAGTGCT
TCTTAAAAACAAGAAGCTAAAGGCCCATCAGACACCTGTCGACATCCTCAAACAAAAGGCTCATCAGTTA
GCATCCATGCAAACACAGGCTTTCACTGGTGGGAATGCCAACCCGCCACCTGCCAGTAATGAGGAAGAGG
AAGACGAGGAGGACGAATATGATTATGATTACGAGTCCCTGTCTGATGACAACATTCTGGAAGACAGACC
TGAGAACAAATCATGTGCCGACAAGCTTCAGTTTGAGTACAATGAAGAAGTCCCCAAGAGGATAAAGAAA
GCAGACAACTCCTCCGGCAATAAGGGAAAGGTCTATGACATGGAACTGGGAGAGGAGTTTTACCTTCCTC
AGAACAAAAAAGAAAGCAGGCAGATTGCACCGGAGCTCTCTGACCTCGTCATCTATTGCCAGGCGGTAAA
ATTTCCAGGCCTGTCAACTCTAAATTCATCTGGCTCTGGCAGAGGGAAAGAAAGGAAAAGCAGGAAGTCC
ATTTTTGGCAACAATCCCGGCAGAATGAGCCCAGGGGAGACAGCGTCATTTAACAGAACATCTGGAAAAA
GTTCTTGTGAAGGAATTCGACAAATCTGGGAGGAACCTCCACTCAGCCCAAACACATCCCTCAGCGCCAT
CATTAGAACTCCTAAATGCTATCATATCTCGTCACTGAATGAAAATGCCGCCAAACGTCTGTGTCGCAGG
TATTCTCAGAAACTGATCCAGCACACTGCTTGCCAGCTGCTGAGGACCTACCCGGCAGCGACCAGAATCG
ACTCATCCAATCCCAACCCCCTCATGTTCTGGCTCCATGGGATACAGCTTGTAGCTCTAAACTACCAGAC
GGATGACCTTCCTTTACATTTAAACGCTGCCATGTTTGAGGCGAATGGTGGGTGTGGCTATGTTTTGAAG
CCCCCTGTCCTGTGGGATAAAAGCTGTCCCATGTATCAGAAGTTTTCACCACTGGAAAGAGACCTGGACG
CCATGGATCCTGCTACCTATTCTTTAACCATCATCTCTGGCCAGAATGTGTGTCCTAGTAACAGCACCGG
AAGTCCGTGCATTGAAGTTGACGTCTTGGGCATGCCCCTGGACAGCTGCCATTTCCGCACAAAACCCATC
CACCGAAACACTCTGAACCCCATGTGGAATGAGCAGTTTCTCTTCCGGGTTCACTTTGAAGATCTTGTAT
TTCTTCGTTTTGCAGTTGTGGAGAACAACAGTTCGGCGATAACGGCTCAGAGAATCATTCCACTCAAAGC
TTTAAAGAGAGGATATCGGCATCTTCAGCTGCGGAACCTCCACAACGAGATCTTGGAAATCTCTAGCTTA
TTCATAAACAGCAGGAGGATGGAAGATAATCCCTCTGGCAGTACCCGGCCAGCCTCTTTGATGTTCAATA
CTGAAGAGAGGAAATGTTCTCAGACTCACAAAGTCACAGTGCATGGTGTCCCAGGTCCAGAGCCCTTTGC
TGTTTTCACAATAAACGAAGGCACCAAGGCAAAGCAGCTTCTCCAACAGATTTTGGCAGTTGACCAGGAC
ACCAAACTTACAGCCGCAGACTATTTTCTGATGGAAGAGAAGCATTTTATATCTAAGGAAAAGAATGAAT
GCAGGAAACAACCATTCCAGAGAGCTGTGGGCCCAGAGGAAGATATTGTGCAGATTTTAAACAGCTGGTT
CCCAGAAGAAGGCTACGTAGGCAGGATTGTCTTAAAGCCCCAACAAGAAACCCTAGAAGAGAAAAACATT
GTCCACGATGACAGAGAGGTGATCTTGAGCTCAGAAGAGGAGAGTTTCTTTGTCCAAGTGCACGATGTCT
CTCCAGAGCAGCCTCGAACTGTCATCAAAGCCCCACGTGTCAGCACTGCACAGGATGTGATTCAGCAGAC
CTTGTGCAAAGCCAAATATTCCTACAGCATCCTGAACAACCCCAATCCGTGTGACTATGTGCTTTTGGAA
GAGGTAATGAAAGATGCACCCAATAAGAAGTCTTCTACCCCGAAGTCCTCACAGAGGATCCTCTTGGACC
AAGAATGTGTGTTTCAAGCCCAAAGCAAGTGGAAGGGTGCAGGGAAATTCATCCTTAAGCTAAAGGAGCA
GGTGCAGGCATCCAGAGAAGATAAAAGGAGGGGCATCTCCTTTGCAAGTGAACTCAAGAAACTCACGAAG
TCCACTAAGCAAACCCGAGGACTCACCTCGCCTCCTCAGCTTGTGGCCTCAGAAAGTGTGCAAAGCAAGG
AGGAGAAACCCATGGGTGCCTTGGCCTCTGGTGACACAGCGGGCTACCAGTCGTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_053758
Insert Size 6846 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_053758.2, NP_446210.1
RefSeq Size 8355 bp
RefSeq ORF 6846 bp
Locus ID 114633
UniProt ID Q99P84
Gene Summary one of four classes of phospholipase C; directly regulated by Ras [RGD, Feb 2006]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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