Rfx7 (NM_001127490) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Rfxdc2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN214947 representing NM_001127490 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGAGGAACAACAACAGCCGCCACCACAGCAGCTCGATGCCCATCAGCAGCTTCCCCTCAGCGCCC CCAACCCCGGGGTGGCTCTGCCAGCCCTTGTGCCCGGGCTGCCAGGGACAGAGGCCAGCGCTCTGCAACA CAAGATCAAGAACTCCATCTGCAAAACTGTACAATCTAAAGTGGACTGCATTTTGCAAGAAGTTGAGAAG TTTACAGACCTAGAGAAACTCTACCTCTACCTTCAACTGCCTTCTGGTCTCAGCAGTGCAGAGAAAAGTG ATCAGAATGCCATGTCATCTAGCCGGGCACAGCAAATGCATGCCTTTTCCTGGATTCGAAATACCTTAGA GGAGCATCCTGAGACTTCACTCCCCAAACAAGAAGTCTATGATGAATACAAGAGCTATTGTGACAATCTT GGCTACCATCCATTAAGTGCTGCTGACTTTGGAAAAATCATGAAGAACGTCTTTCCAAACATGAAGGCAC GCCGTTTGGGCACTAGAGGCAAATCTAAATATTGCTACAGTGGACTAAGGAAAAAAGCTTTTGTTCATAT GCCAACACTGCCCAACCTTGACTTTCATAAAACTGGAGATGGGTTGGAAGGAGTTGAACCATCTGGGCAG CTTCAAAATATTGATGAGGAAGTTATCTCTTCTGCTTGCCGTCTTGTGTGTGAGTGGGCCCAGAAAGTGT TAAGCCAGCCATTTGACACAGTCTTGGAATTAGCCCACTTCCTTGTAAAGAGTCATTATATAGGCACCAA GTCAATGGCAGCTCTGACTGTGATGGCAGCAGCACCAGCAGGACTTAAAGGAATTTCCCAGCCTTCTGCT TTTATACCTACAGCTGAAAGTAATTCCTTTCAACCTCAAGTAAAGACTTTGTCATCTCCAATTGATGCTA AACAGCAGTTGCAACGGAAAATTCAAAAAAAGCAGCAAGAACAGAAACTACAGTCCCCATTACCAGGAGA ATCCTCAGCAAAAAAAACAGAAGGCACTACAGCCAACGGAGTGGCTAATCTACCCAATGGGAACCCTGCG ATCCTTTCTCCCCAGCCCATTGGGATTGTGGTAGCAGCTGTCCCTAGTCCCATTCCGGTCCAGCGGACGA GGCAGTTAGTAACTTCACCCAGTCCGATGAATTCTTCTGATGGCAAGGTTCTTCCCCTCAACGTGCAGGT AGTCACTCAGCACATGCAGTCTGTGAAACAGACACCAAAGACTCCTCAGAACGTCCCAGCCAGTCCTGGT GGGGACCGTTCTGCCAGGCACCGCTACCCTCAGATCTTACCCAAGCCAGCAAACACCAGTGCGCTCACCA TCCGCTCTCCTACGACTGTGCTGTTTACCAGCAGTCCCATCAAAACTGCTGTGGTACCTGCGTCACACAT GAGTTCTCTAAATGTGGTGAAAATGACAACAATATCCCTCACACCCAGTAACAGTAATGCGCCTCTTAAA CATTCTGCCTCCGTCAGCAATGCTACTGGAACAACAGAGGACTCCAGGAGCATCCCTCAGATCAAGAATG GTTCTGTTGTTTCTCTCCAGTCCCCTGGGTCCAGGGCCAGCAGCACTGGGGGAACCTCTGCTGTGGAAGT CAAAATGGAACCAGAAGGTTCATCAGATGAGCATCCTCTGCAGTGCCAAGAGAACGCTGAGGGGACTAAA GCTCCTCTGGCCACATCTAGTGCCCTTTGGGGACAGAAAAGTAATACAGATGGAACAGTATCAAAACCTT CTAATGAAGGAGTCACAGAAGTGAAAACAACTAAGGTCTGTGACCAGAGGGCCAAGTGTAAAAGTCGCTG TAATGAAACTCTGCCAGGCATCTCAGCAGGCAATAATCAAAGCACTGTTACTCTCTCAGTTGCTACTCAG AACTTACCCTTCACCAGCACCAGCTCACCATCTAATGGTGACTCAGTAAATAAAGACCCTAAATTATGCA CTAAAAGTCCAAGAAAACGACTGTCTGCTACACTGCAAGAGTCTCAGGTGCCTCCTGTAAAGAAACCAAT TGTAGAACAGCTTTCTGCAGTTACTACCGAAGGTCAGAAACCAGGCACTGTTAAAAAGGACCAAAAGGTT CCACATTCAGGGAAAACAGAAAGCTCAACAGCAGGTGCTCAGCCTCCTAACAAGGTGTCACTCAGTGTCA GCTCACACATTGTAGAGGATCAGCCCTTGAACCCTACTCTTGTCGCCAGTGAATCAGCTCTGGAGCAGCA GACAACGCCATCATCGTCTCCGGATGTAAAAGTAAAACTTGAAGGGAGTGTCTTTCTTTTGGACCGTGAG TCAAACTCAGATGGCAGCTTTAATCGTAACGAATGGCAACAAGTTACTAAGGATTCTGACTTCATATCTG CCAGCTGTGAGCAACAGCAAGACATTAGTGTGATGACAATTCCTGAGCATCCTGACATCCATGATTTAGA GAAGTCTGTTTGGGAATTAGAAGGGATGCCACAGGACACATACAGCCAGCAGCTCCATAGCCAGATACCA GAATCTTCTCTGAATGAAATACAAGCACAGTCTTCAGATCAGTTACCGTTGCAGTCGGAACTGAAAGAGT TTGAGTCTTCTGTTTCCCAGACAAATGAAAGCTACTTTCCTTTTGATGATGAACTTACACAAGATAGCAT TGTGGAAGAGCTGGTGCTTATGGAGCAGCAGATGTCAATGAATAACTCCCATTCTTATGGTAACTGCTTG GGAATGACCCTTCAGAGTCAGTCAGTAACTCCAGGAGCTCCAATGTCATCTCATGGTTCCAGCTCTCACT TCTATCATCCAATCCATAGCAATGGCACTCCAATTCACACACCTACACCCACACCCACTCCTACACCAAC TCCGACCCCAACTCCAACCCCAACATCTGAAATGATTGCTGGGTCTCAGAGTCTGTCACGGGAGAGCCCT TGTTCCAGGCTTGCCCAGACAACACCTGTGGATAGTGCTTTAGGAAGTAGCCGACACACACCCATTGGTA CTCCTCATTCTAATTGCAGCAGTAGCGTCCCTCCAAGCCCTGTTGAATGCAGGAATCCATTTGCATTCAC CCCAATAAGCTCCAGTATGGCATATCACGATGCCAGCATTGTCTCAAGCAGTCCTGTAAAACCAATGCAA AGACCCATGGCCACTCACCCTGATAAAACCAAGCTTGAATGGATGAATAATGGGTATGGTGGGGTTGGTA ACTCATCAGTTTCTGGTCACGGCATTCTCCCAAGCTATCAGGAACTAGTAGAAGATCGTTTCAGGAAACC TCATGCTTTTGCTGTGCCCGGACAGTCGTATCAGTCTCAGTCCAGACACCATGACACTCATTTTGGTCGT TTGACTCCCGTCTCTCCTGTGCAGCATCAAGGTGCCACTGTAAACACCAACAAGCAAGAAGGGTTTGCAG TCCCCGCTCCTCTTGATAATAAAGGAACTAATTCATCTGCCAGCAGCAACTTCAGGTGCCGGAGTGTAAG CCCTGCTGTCCATCGCCAACGGAATCTTAGTGGAAGCACCCTCTATCCTGTGTCTAATATCCCACGGTCT AATGTAACCCCCTTTGGAAGTCCAGTGACCCCAGAAGTTCATGTTTTCACAAATGTTCACACAGATGCAT GTGCCAACAACATAGCTCAAAGAAGTCAGTCAGTTCCATTAACAGTCATGATGCAGACAGCCTTCCCTAA TGCTCTGCAGAAGCAAACAAACAGTAAAAAAATCACTAATGTTTTGTTGAGTAAACTCGATTCTGACAAT GATGATGCAGTGAGAGGTTTGGGAATGAACAACGTGCCCTCCAATTACACAGCCCGGATGAATCTCACTC AGATTTTGGAAACTTCCCCTGTTTTTCCTAGTGCCAATTCACAAAATATGATCGATTCCAGCACTTCTGT TTATGAATTCCAAACACCATCTTACCTCACCAAAAGTAATAGCACCGATCAGATCAGTTTTTCTCCTGGA GATAATCAAGCACAATCTGAAATTGGAGAACAGCAGTTAGATTTCAATAGCACTGTTAAAGACCTGTTGA GTGGAGACAACCTGCAAACCAGCCAGCAGCTGGGAGGTCAGGTAGCATCTGATCTCACCAACACTGCATC TGATTTCCCTAGCGACATCAGGTTGTCTTCTGACCTCTCAGGCAGCATCAATGATTTGAACACCTTAGAC CCAAATCTACTGTTTGATCCAGGTCGTCAGCAGGGACAGGATGATGAAGCCACACTGGAAGAATTAAAGA ATGACCCACTGTTTCAACAGATTTGCAGTGAGTCAATGAACTCTATGACTTCATCAGGTTTTGAATGGAT AGAAAGCAAGGACCATCCTACTGTTGAAATGTTGGGATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001127490
Insert Size	4380 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001127490.1, NP_001120962.1
RefSeq Size	7998 bp
RefSeq ORF	4380 bp
Locus ID	315804

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