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Fbxo9 (NM_001011998) Rat Untagged Clone
CAT＃: RN214866
Fbxo9 (untagged ORF) - Rat f-box protein 9 (Fbxo9), (10 ug)
CNY 5,610.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN214866 representing NM_001011998
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGAAGCGGAGGAAGATTGTCATTCTGATGCTGTCAGAGTAGGCGATGAAGGACATGAGAGTCCGG CTGAGAGAGACCTGCAGGCGCAGCTCCAGATGTTCAGAGCTCAGTGGATGTTTGAACTTACCCCAGGCGT AGGTTCCAGTAATGTGGAAAGCCGGCCTTGCAGAGCAGGGAGAAGCTCTATACTGAAAGCAGCTGCAGAC AAAGGAAGACAAGAATTGGCAAAGGAGGAAAAGGCTCGAGAACTCTTCCTGAAGGCAGTAGAGGAAGAAC AAAATGGAGCTCTCTATGAAGCCATCAAGTTCTACCGTAGGGCTATGCAGCTAGTACCTGATATTGAGTT CAAGATCACTTATACCCGGTCTCCAGATGGCGATGGCGTTGGAAGCAGCTACATCGAAGATAATGAGGAC GCCAGCAAGATGGCCGATCTCCTGTCGTACTTCCAGCAGCAGCTCACATTTCAGGAGTCTGTGCTCAAAC TCTGTCAGCCTGAGCTTGAGACCAGTCAGACTCACATATCAGTCCTGCCTATGGAGGTGCTGATGTACAT CTTCCGATGGGTGGTGTCAAGTGACTTGGACCTCAGATCGTTAGAGCAGTTGTCACTGGTGTGCAGAGGA TTCTATATCTGTGCCAGAGACCCTGAAATATGGCGTCTGGCTTGCTTGAAAGTGTGGGGCAGAAGCTGTA TGAAACTTGTCCCATTCTCATCCTGGAGAGAGATGTTTCTAGAACGGCCTCGTGTTCGGTTTGACGGCGT GTACATCAGTAAAACCACATATATTCGCCAGGGAGAGCAGTCTCTTGATGGTTTCTATAGAGCCTGGCAC CAAGTGGAATATTACAGATACATAAGATTCTTTCCTGATGGCCATGTGATGATGTTGACAACCCCTGAGG AGCCTCCATCCATTGTTCCCCGTTTAAGAACCAGGAACACCAGAACGGATGCAATTCTGCTGGGTCATTA TCGCTTGTCACAAGATGCAGACAATCAGACCAAAGTATTTGCTGTGATAACTAAGAAAAAAGAAGAAAAG CCACTTGACCATAAGTACAGGTATTTTCGCCGTGTTCCTGTCCAAGAGGCAGATCACAACTTTCATGTGG GGCTGCAGCTGTGCTCCAGTGGCCACCAGAGGTTCAACAAACTCATCTGGATCCACCACTCTTGTCACAT CACTTACAGATCGACTGGTGAGACTGCAGTGAGTGCTTTTGATATTGACAAGATGTACACCCCCTTGTTC TTCGCCAGAGTGAGAAGCTACACGGCGTTCTCCGAGAGGCCTCTGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001011998 |
| Insert Size | 1308 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001011998.1, NP_001011998.1 |
| RefSeq Size | 2155 bp |
| RefSeq ORF | 1308 bp |
| Locus ID | 300849 |
| UniProt ID | Q5U2X1 |
| Gene Summary | Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of TTI1 and TELO2 in a CK2-dependent manner, thereby directly regulating mTOR signaling. SCF(FBXO9) recognizes and binds mTORC1-bound TTI1 and TELO2 when they are phosphorylated by CK2 following growth factor deprivation, leading to their degradation. In contrast, the SCF(FBXO9) does not mediate ubiquitination of TTI1 and TELO2 when they are part of the mTORC2 complex. As a consequence, mTORC1 is inactivated to restrain cell growth and protein translation, while mTORC2 is activated due to the relief of feedback inhibition by mTORC1 (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR214866 | Fbxo9 (Myc-DDK-tagged ORF) - Rat f-box protein 9 (Fbxo9), (10 ug) |
CNY 5,610.00 |
|
| RR214866L3 | Lenti ORF clone of Fbxo9 (Myc-DDK-tagged ORF) - Rat f-box protein 9 (Fbxo9), (10 ug) |
CNY 7,510.00 |
|
| RR214866L4 | Lenti ORF clone of Fbxo9 (mGFP-tagged ORF) - Rat f-box protein 9 (Fbxo9), (10 ug) |
CNY 8,270.00 |
