Selenoo (NM_001085485) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Synonyms	RGD1311907; Selo
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN214580 representing NM_001085485 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTCTTTCAGGGCCGCGTTCGGGGCCTCGCTCGCGGTTGCCCGAACCCGGCCACAGTGTGTCGGCC TCGAGCTTCAGTCGTCCGCGCCCTGGTCCGCCTGGGCTGCTGCCATGGAGCCCACGCCGCGTTGGCTGGC GAGGCTGCGCTTCGACAACCGTGCCCTGCGTGCGCTGCCGGTAGAGACGCCACCGCCCGGGCCGGAGGAC TCCTTGTCCACCCCGCGTCCGGTGCCGGGAGCCTGCTTCAGCCGTGCACGGCCGGCCCCGCTGCGGCAGC CACGTCTAGTGGCGCTGTCAGAGCCCGCACTGGCGCTGCTGGGGCTCGAGGTCAGCGAGGAGGCGGAGGT CGAGGCCGAAGCTGCGCTCTTCTTCAGCGGCAATGCGCTGCTGCCGGGCACCGAGCCCGCCGCGCACTGC TACTGTGGACACCAGTTCGGTCAGTTCGCTGGGCAGTTGGGCGACGGTGCCGCCATGTACCTGGGCGAGG TGTGCACAGCGGCCGGCGAGCGCTGGGAGCTGCAGCTCAAAGGCGCCGGCCCCACGGCCTTCTCCAGACA AGCTGATGGTCGCAAAGTCCTGCGGTCAAGCATCCGTGAGTTCCTGTGCAGCGAAGCCATGTTTCACCTG GGGATCCCCACCACGAGGGCCGGGGCCTGCGTTACGTCTGAGTCTACAGTGATGCGAGACGTGTTCTATG ATGGTAATCCAAAATATGAAAAGTGCACGGTTGTGTTGCGTATAGCTCCCACTTTCATAAGATTCGGCTC CTTTGAAATTTTTAAGCCTCCTGATGAGCTCACAGGGCGTGCAGGCCCTAGTGTAGGACGAAATGATATC CGAGTGCAGATGCTTGACTATGTCATCAGCTCTTTCTACCCTGAAATCCAGGCTGCCCACACCTGCGACA CAGACAACATACAGAGGAACGCTGCCTTTTTCAGAGAGGTGACAAGGCGAACAGCACGGATGGTGGCTGA ATGGCAGTGTGTCGGCTTTTGCCATGGAGTGCTCAACACTGACAACATGAGCATCGTGGGCCTCACGATC GACTATGGACCCTTTGGCTTCCTGGATAGGTATGACCCTGACCATGTGTGTAACGCCTCTGACAATGCTG GGCGCTACACATACAGTAAGCAGCCACAGGTGTGCAGGTGGAACCTGCAGAAACTAGCTGAAGCCCTGGA GCCTGAGCTGCCGCTTGTGCTAGCTGAGGCCATTCTCAAAGAGGAGTTTGACACAGAGTTCCAAAGGCAC TATCTACAGAAGATGCGTAAGAAGCTGGGCCTTGTTCGCGTGGAGAAGGAAGATGAGACACTTGTGGCCA AACTTCTAGAGACTATGCATCAGACTGGCGCTGACTTCACTAACACCTTCTGTGTTCTGAGCTCCTTCCC GGCTGAACCGTCAGACACAGCAGAGTTCCTGACCCAGCTGACCTCCCAGTGTGCCTCTCTGGAAGAGCTA AAGCTTGCCTTCAGACCCCAGATGGATCCCCGGCAGCTCTCTATGATGCTGATGCTGGCACAGTCGAACC CACAGCTCTTTGCACTCATTGGCACTCAAGCAAATGTCACAAAGGAGCTGGAACGTGTGGAGCATCAGTC ACGGCTGGAACAGCTGAGCCCCTCTGAACTGCAGAGCAAGAACAGAGACCACTGGGAAACCTGGCTACAG GAATACAGAGAGCGTCTGGACAAGGAGAAGGAGGGTGTCGGAGACATTGCTGCCTGGCAGGCAGAACGTG TACGCATCATGCATGCCAACAACCCCAAGTACGTCCTAAGGAACTATATTGCACAGAAAGCCATTGAAGC TGCAGAAAATGGAGACTTTTCAGAGGTGCGACGTGTCCTGAAGCTGCTAGAGTCTCCTTACCACAGTGAA GAGGAGGCCACAGGCCCTGAGGCAGTGGCGAGGACCACTGACGAGCAGTCTTCCTACAGTAGCAGGCCTC CACTCTGGGCAGCAGAACTCTGCGTAACATGATCCTCATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001085485
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). The expression of this clone is not guaranteed due to the nature of selenoproteins.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001085485.1, NP_001078954.1
RefSeq Size	2481 bp
RefSeq ORF	2001 bp
Locus ID	315216
Gene Summary	This gene encodes a selenoprotein that is localized to the mitochondria. It is the largest mammalian selenoprotein, containing the rare amino acid selenocysteine (Sec). Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. The exact function of this selenoprotein is not known, but it is thought to have redox activity. [provided by RefSeq, Jan 2017]

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