Gne (NM_053765) Rat Untagged Clone

Name: Gne (NM_053765) Rat Untagged Clone
SKU: RN214376
Price: 9220.00

CAT＃: RN214376

Gne (untagged ORF) - Rat glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (Gne), (10 ug)

Datasheet

SDS

"NM_053765" in other vectors (3)

CNY 9,220.00

货期*

6周

规格

Product images

经常一起买 (3)

TF81001

TurboFectin Transfection Reagent (1 mL in 1 vial)

CNY 4,090.00

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Uae1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN214376 representing NM_053765 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGAAGAACGGGAATAACCGGAAGCTTCGGGTTTGCGTTGCCACCTGCAACCGAGCCGATTACTCCA AACTGGCCCCCATCATGTTCGGCATTAAGACGGAGCCTGCGTTCTTCGAGCTCGACGTGGTGGTGCTGGG CTCTCACCTGATCGACGACTACGGAAACACATACCGCATGATTGAGCAGGACGACTTTGACATCAACACC AGGCTACACACGATTGTTAGAGGGGAAGACGAAGCAGCCATGGTAGAGTCAGTGGGCCTAGCGCTAGTGA AGCTACCGGATGTCCTCAACCGCCTGAAGCCTGACATCATGATTGTTCACGGAGACCGATTTGACGCCCT CGCTCTGGCTACATCTGCTGCCCTGATGAACATCCGCATCCTTCACATTGAAGGAGGAGAGGTCAGCGGG ACTATTGATGACTCTATCAGACACGCCATAACAAAACTGGCTCACTACCACGTGTGCTGCACCAGGAGTG CAGAGCAACACCTGATCTCCATGTGTGAGGACCACGACCGCATCCTTTTGGCTGGCTGCCCTTCCTATGA CAAACTGCTCTCAGCCAAGAATAAAGACTATATGAGCATCATTCGGATGTGGCTAGGTGATGATGTAAAA TGTAAAGATTACATTGTTGCCCTGCAACACCCGGTGACCACCGACATTAAGCATTCCATAAAGATGTTCG AACTGACACTGGATGCTCTTATCTCATTTAACAAGAGGACCCTAGTTCTGTTTCCAAATATCGATGCAGG CAGCAAGGAGATGGTTCGAGTGATGCGGAAGAAGGGCATCGAGCATCACCCCAATTTCCGCGCAGTCAAG CACGTCCCGTTTGACCAGTTCATTCAGCTGGTCGCCCACGCTGGCTGCATGATTGGGAATAGCAGCTGTG GAGTGCGTGAGGTTGGCGCCTTTGGAACCCCTGTGATCAACCTGGGCACGCGGCAGATAGGAAGAGAAAC GGGGGAGAATGTTCTTCATGTCCGGGATGCTGACACCCAAGACAAAATATTACAAGCACTACACCTCCAG TTCGGTAAACAGTACCCTTGCTCAAAGATATATGGGGATGGAAATGCTGTTCCAAGGATTTTAAAGTTTC TCAAATCCATCGACCTTCAAGAGCCACTACAGAAGAAATTCTGCTTCCCTCCCGTGAAGGAGAACATCTC TCAGGATATTGACCATATCCTCGAAACTCTGAGTGCCTTGGCTGTTGATCTCGGGGGGACGAATCTGAGA GTGGCGATAGTTAGCATGAAGGGTGAAATAGTTAAGAAGTACACCCAGTTCAATCCTAAAACCTATGAGG AAAGGATTAGTCTAATCCTGCAGATGTGTGTGGAAGCGGCAGCAGAAGCCGTGAAGCTCAATTGCAGAAT TCTGGGAGTAGGCATCTCCACAGGTGGCCGTGTGAATCCCCAGGAAGGAGTTGTGCTGCACTCGACCAAG CTGATACAGGAGTGGAACTCTGTGGACCTCAGGACACCACTCTCCGACACCCTGCATCTCCCCGTGTGGG TGGACAACGACGGCAACTGCGCTGCCATGGCGGAGAGGAAGTTTGGCCAAGGAAAAGGACAGGAGAACTT TGTGACGCTCATCACAGGGACAGGGATCGGTGGGGGAATCATCCACCAGCACGAGCTGATCCACGGCAGC TCCTTCTGTGCGGCAGAGCTTGGCCACCTCGTGGTGTCTCTGGATGGTCCTGACTGCTCCTGTGGAAGCC ATGGGTGCATTGAAGCCTACGCCTCTGGAATGGCCTTGCAGAGGGAAGCAAAGAAGCTCCACGACGAGGA CCTGCTCTTGGTGGAAGGGATGTCAGTGCCAAAAGACGAAGCTGTGGGCGCCCTCCATCTCATCCAAGCC GCCAAGCTGGGCAACGTGAAGGCCCAGAGCATCTTACGGACAGCTGGAACTGCTTTGGGACTCGGAGTTG TGAATATCCTCCACACTATGAATCCTTCCCTGGTGATCCTGTCTGGAGTCCTGGCTAGTCACTACATCCA CATTGTGAGGGACGTCATCCGCCAGCAAGCCCTGTCCTCCGTGCAGGATGTGGATGTAGTGGTTTCAGAC TTGGTTGACCCGGCCCTGCTTGGTGCGGCCAGCATGGTTCTGGACTACACGACCCGCAGGATCCACTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_053765
Insert Size	2169 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_053765.2, NP_446217.1
RefSeq Size	2843 bp
RefSeq ORF	2169 bp
Locus ID	114711
UniProt ID	O35826
Gene Summary	bifunctional enzyme, catalyzes the first two steps of biosynthesis of Neu5Ac (UDP-GlcNAc 2-epimerase and ManNAc kinase), precursor of sialic acids [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
RR214376	Gne (Myc-DDK-tagged ORF) - Rat glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (Gne), (10 ug)	10 ug 产品选项验证失败所有选项都是必需的	CNY 9,220.00
RR214376L3	Lenti ORF clone of Gne (Myc-DDK-tagged ORF) - Rat glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (Gne), (10 ug)	10 ug 产品选项验证失败所有选项都是必需的	CNY 11,020.00
RR214376L4	Lenti ORF clone of Gne (mGFP-tagged ORF) - Rat glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (Gne), (10 ug)	10 ug 产品选项验证失败所有选项都是必需的	CNY 12,160.00

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Expression Plasmids

Lentiviral Particles

CRISPR

RNAi

Proteins

Antibodies