Gne (NM_053765) Rat Untagged Clone

CAT#: RN214376

Gne (untagged ORF) - Rat glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (Gne), (10 ug)



  "NM_053765" in other vectors (3)

CNY 9,220.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Synonyms Uae1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN214376 representing NM_053765
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGAAGAACGGGAATAACCGGAAGCTTCGGGTTTGCGTTGCCACCTGCAACCGAGCCGATTACTCCA
AACTGGCCCCCATCATGTTCGGCATTAAGACGGAGCCTGCGTTCTTCGAGCTCGACGTGGTGGTGCTGGG
CTCTCACCTGATCGACGACTACGGAAACACATACCGCATGATTGAGCAGGACGACTTTGACATCAACACC
AGGCTACACACGATTGTTAGAGGGGAAGACGAAGCAGCCATGGTAGAGTCAGTGGGCCTAGCGCTAGTGA
AGCTACCGGATGTCCTCAACCGCCTGAAGCCTGACATCATGATTGTTCACGGAGACCGATTTGACGCCCT
CGCTCTGGCTACATCTGCTGCCCTGATGAACATCCGCATCCTTCACATTGAAGGAGGAGAGGTCAGCGGG
ACTATTGATGACTCTATCAGACACGCCATAACAAAACTGGCTCACTACCACGTGTGCTGCACCAGGAGTG
CAGAGCAACACCTGATCTCCATGTGTGAGGACCACGACCGCATCCTTTTGGCTGGCTGCCCTTCCTATGA
CAAACTGCTCTCAGCCAAGAATAAAGACTATATGAGCATCATTCGGATGTGGCTAGGTGATGATGTAAAA
TGTAAAGATTACATTGTTGCCCTGCAACACCCGGTGACCACCGACATTAAGCATTCCATAAAGATGTTCG
AACTGACACTGGATGCTCTTATCTCATTTAACAAGAGGACCCTAGTTCTGTTTCCAAATATCGATGCAGG
CAGCAAGGAGATGGTTCGAGTGATGCGGAAGAAGGGCATCGAGCATCACCCCAATTTCCGCGCAGTCAAG
CACGTCCCGTTTGACCAGTTCATTCAGCTGGTCGCCCACGCTGGCTGCATGATTGGGAATAGCAGCTGTG
GAGTGCGTGAGGTTGGCGCCTTTGGAACCCCTGTGATCAACCTGGGCACGCGGCAGATAGGAAGAGAAAC
GGGGGAGAATGTTCTTCATGTCCGGGATGCTGACACCCAAGACAAAATATTACAAGCACTACACCTCCAG
TTCGGTAAACAGTACCCTTGCTCAAAGATATATGGGGATGGAAATGCTGTTCCAAGGATTTTAAAGTTTC
TCAAATCCATCGACCTTCAAGAGCCACTACAGAAGAAATTCTGCTTCCCTCCCGTGAAGGAGAACATCTC
TCAGGATATTGACCATATCCTCGAAACTCTGAGTGCCTTGGCTGTTGATCTCGGGGGGACGAATCTGAGA
GTGGCGATAGTTAGCATGAAGGGTGAAATAGTTAAGAAGTACACCCAGTTCAATCCTAAAACCTATGAGG
AAAGGATTAGTCTAATCCTGCAGATGTGTGTGGAAGCGGCAGCAGAAGCCGTGAAGCTCAATTGCAGAAT
TCTGGGAGTAGGCATCTCCACAGGTGGCCGTGTGAATCCCCAGGAAGGAGTTGTGCTGCACTCGACCAAG
CTGATACAGGAGTGGAACTCTGTGGACCTCAGGACACCACTCTCCGACACCCTGCATCTCCCCGTGTGGG
TGGACAACGACGGCAACTGCGCTGCCATGGCGGAGAGGAAGTTTGGCCAAGGAAAAGGACAGGAGAACTT
TGTGACGCTCATCACAGGGACAGGGATCGGTGGGGGAATCATCCACCAGCACGAGCTGATCCACGGCAGC
TCCTTCTGTGCGGCAGAGCTTGGCCACCTCGTGGTGTCTCTGGATGGTCCTGACTGCTCCTGTGGAAGCC
ATGGGTGCATTGAAGCCTACGCCTCTGGAATGGCCTTGCAGAGGGAAGCAAAGAAGCTCCACGACGAGGA
CCTGCTCTTGGTGGAAGGGATGTCAGTGCCAAAAGACGAAGCTGTGGGCGCCCTCCATCTCATCCAAGCC
GCCAAGCTGGGCAACGTGAAGGCCCAGAGCATCTTACGGACAGCTGGAACTGCTTTGGGACTCGGAGTTG
TGAATATCCTCCACACTATGAATCCTTCCCTGGTGATCCTGTCTGGAGTCCTGGCTAGTCACTACATCCA
CATTGTGAGGGACGTCATCCGCCAGCAAGCCCTGTCCTCCGTGCAGGATGTGGATGTAGTGGTTTCAGAC
TTGGTTGACCCGGCCCTGCTTGGTGCGGCCAGCATGGTTCTGGACTACACGACCCGCAGGATCCACTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_053765
Insert Size 2169 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_053765.2, NP_446217.1
RefSeq Size 2843 bp
RefSeq ORF 2169 bp
Locus ID 114711
UniProt ID O35826
Gene Summary bifunctional enzyme, catalyzes the first two steps of biosynthesis of Neu5Ac (UDP-GlcNAc 2-epimerase and ManNAc kinase), precursor of sialic acids [RGD, Feb 2006]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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