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Agxt (NM_030656) Rat Untagged Clone
CAT＃: RN214253
Agxt (untagged ORF) - Rat alanine-glyoxylate aminotransferase (Agxt), (10 ug)
CNY 3,990.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | AGT; Spat; SPT |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN214253 representing NM_030656
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCCGGATGTTGGCCAAGGCCAGTGTGACGCTGGGCTCCAGGGCAGCAAGTTGGGTACGGAACATGG GCTCGCACCAGCTGCTGGTGCCACCCCCAGAGGCCCTGAGCAAGCCCCTGTCAATTCCTAAGAGGCTCCT GTTGGGTCCGGGACCCTCCAACCTGGCTCCTCGTGTGCTAGCAGCTGGAAGTCTGAGGATGATTGGCCAC ATGCAAAAAGAGATGTTTCAGATCATGGATGAGATCAAGCAGGGCATCCAGTATGTGTTCCAGACCAGGA ACCCCCTCACACTGGTTGTCAGCGGCTCAGGACATTGTGCCATGGAGACTGCCCTGTTCAACCTCCTGGA GCCTGGGGACTCCTTTCTTGTGGGAACCAATGGCATCTGGGGGATACGGGCTGCAGAGATCGCTGAGCGG ATTGGAGCCCGTGTGCACCAGATGATCAAGAAGCCTGGAGAACATTACACACTGCAGGAGGTGGAGGAGG GCCTGGCTCAGCATAAACCAGTGTTGCTGTTCCTGACCCACGGGGAGTCATCCACTGGTGTGCTGCAGCC CCTGGATGGTTTCGGGGAGCTCTGCCACAGGTATCAGTGCCTACTCCTGGTGGACTCGGTGGCATCATTG GGCGGAGTCCCTATCTACATGGACCAACAAGGCATCGACATCTTGTACTCTGGCTCTCAGAAGGTCCTGA ATGCCCCACCAGGGATCTCCCTCATCTCCTTCAACGACAAGGCCAAATCCAAAGTCTACTCCCGGAAGAC AAAGCCAGTCTCCTTCTACACAGACATCACTTATTTGTCCAAGTTGTGGGGCTGTGAGGGCAAGACCAGA GTAATTCATCATACGTTGCCTGTCATCAGCTTATACTGCCTGAGGGAGAGCCTAGCACTCATTTCAGAGC AGGGCCTGGAGAATTCCTGGCGGCGTCACAGGGAGGCTACAGCACATCTGCACAAGTGCCTGCGGGAGTT GGGCTTAAAGTTCTTTGTGAAGGACCCGGAAATCCGGCTACCTACAATCACCACCGTGACCGTGCCTGCC GGCTACAACTGGAGGGACATCGTCAGCTACGTGCTGGACCACTTCAACATTGAAATCTCTGGTGGTCTTG GGCCCTCTGAGGATAAGGTGCTGCGGATTGGCCTCCTGGGCTACAACGCCACCACAGAGAATGCGGACCG TGTAGCGGAGGCCCTGAGGGAGGCCCTGCAACATTGTCCTAAGAATAAATTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_030656 |
| Insert Size | 1245 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_030656.2, NP_085914.2 |
| RefSeq Size | 1628 bp |
| RefSeq ORF | 1245 bp |
| Locus ID | 24792 |
| UniProt ID | P09139 |
| Gene Summary | This gene encodes alanine-glyoxylate aminotransferase, which catalyzes the interconversion of L-alanine and glyoxylate to pyruvate and glycine. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. The longer transcript variant includes an upstream translation start codon and a downstream translation start codon. The upstream start codon initiates the translation of the mitochondrial enzyme precursor while the downstream start codon initiates the translation of the peroxisomal enzyme (see PMID:2332438). [provided by RefSeq, Feb 2013] Transcript Variant: This variant (1) represents the longer transcript. It contains an upstream start codon and a downstream start codon. The upstream start codon initiates the translation of the longest isoform (1), which is a mitochondrial enzyme precursor. The mature peptide, also known as the mitochondrial enzyme, is identical to isoform 3, also known as the peroxisomal enzyme. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR214253 | Agxt (Myc-DDK-tagged ORF) - Rat alanine-glyoxylate aminotransferase (Agxt), (10 ug) |
CNY 3,990.00 |
|
| RR214253L3 | Lenti ORF clone of Agxt (Myc-DDK-tagged ORF) - Rat alanine-glyoxylate aminotransferase (Agxt), (10 ug) |
CNY 6,080.00 |
|
| RR214253L4 | Lenti ORF clone of Agxt (mGFP-tagged ORF) - Rat alanine-glyoxylate aminotransferase (Agxt), (10 ug) |
CNY 6,650.00 |
