Agxt (NM_030656) Rat Untagged Clone

CAT#: RN214253

Agxt (untagged ORF) - Rat alanine-glyoxylate aminotransferase (Agxt), (10 ug)



  "NM_030656" in other vectors (3)

CNY 3,990.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Synonyms AGT; Spat; SPT
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN214253 representing NM_030656
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTCCGGATGTTGGCCAAGGCCAGTGTGACGCTGGGCTCCAGGGCAGCAAGTTGGGTACGGAACATGG
GCTCGCACCAGCTGCTGGTGCCACCCCCAGAGGCCCTGAGCAAGCCCCTGTCAATTCCTAAGAGGCTCCT
GTTGGGTCCGGGACCCTCCAACCTGGCTCCTCGTGTGCTAGCAGCTGGAAGTCTGAGGATGATTGGCCAC
ATGCAAAAAGAGATGTTTCAGATCATGGATGAGATCAAGCAGGGCATCCAGTATGTGTTCCAGACCAGGA
ACCCCCTCACACTGGTTGTCAGCGGCTCAGGACATTGTGCCATGGAGACTGCCCTGTTCAACCTCCTGGA
GCCTGGGGACTCCTTTCTTGTGGGAACCAATGGCATCTGGGGGATACGGGCTGCAGAGATCGCTGAGCGG
ATTGGAGCCCGTGTGCACCAGATGATCAAGAAGCCTGGAGAACATTACACACTGCAGGAGGTGGAGGAGG
GCCTGGCTCAGCATAAACCAGTGTTGCTGTTCCTGACCCACGGGGAGTCATCCACTGGTGTGCTGCAGCC
CCTGGATGGTTTCGGGGAGCTCTGCCACAGGTATCAGTGCCTACTCCTGGTGGACTCGGTGGCATCATTG
GGCGGAGTCCCTATCTACATGGACCAACAAGGCATCGACATCTTGTACTCTGGCTCTCAGAAGGTCCTGA
ATGCCCCACCAGGGATCTCCCTCATCTCCTTCAACGACAAGGCCAAATCCAAAGTCTACTCCCGGAAGAC
AAAGCCAGTCTCCTTCTACACAGACATCACTTATTTGTCCAAGTTGTGGGGCTGTGAGGGCAAGACCAGA
GTAATTCATCATACGTTGCCTGTCATCAGCTTATACTGCCTGAGGGAGAGCCTAGCACTCATTTCAGAGC
AGGGCCTGGAGAATTCCTGGCGGCGTCACAGGGAGGCTACAGCACATCTGCACAAGTGCCTGCGGGAGTT
GGGCTTAAAGTTCTTTGTGAAGGACCCGGAAATCCGGCTACCTACAATCACCACCGTGACCGTGCCTGCC
GGCTACAACTGGAGGGACATCGTCAGCTACGTGCTGGACCACTTCAACATTGAAATCTCTGGTGGTCTTG
GGCCCTCTGAGGATAAGGTGCTGCGGATTGGCCTCCTGGGCTACAACGCCACCACAGAGAATGCGGACCG
TGTAGCGGAGGCCCTGAGGGAGGCCCTGCAACATTGTCCTAAGAATAAATTGTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_030656
Insert Size 1245 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_030656.2, NP_085914.2
RefSeq Size 1628 bp
RefSeq ORF 1245 bp
Locus ID 24792
UniProt ID P09139
Gene Summary This gene encodes alanine-glyoxylate aminotransferase, which catalyzes the interconversion of L-alanine and glyoxylate to pyruvate and glycine. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. The longer transcript variant includes an upstream translation start codon and a downstream translation start codon. The upstream start codon initiates the translation of the mitochondrial enzyme precursor while the downstream start codon initiates the translation of the peroxisomal enzyme (see PMID:2332438). [provided by RefSeq, Feb 2013]
Transcript Variant: This variant (1) represents the longer transcript. It contains an upstream start codon and a downstream start codon. The upstream start codon initiates the translation of the longest isoform (1), which is a mitochondrial enzyme precursor. The mature peptide, also known as the mitochondrial enzyme, is identical to isoform 3, also known as the peroxisomal enzyme.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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