Dgki (NM_198782) Rat Untagged Clone

CAT#: RN214094

Dgki (untagged ORF) - Rat diacylglycerol kinase, iota (Dgki), (10 ug)



  "NM_198782" in other vectors (3)

CNY 16,820.00


货期*
8周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Synonyms rDGKi-3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN214094 representing NM_198782
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATGCTGCGGGAAGGGGCTGCCATTTGCTGCCCCTGCCAGCGGCGCGCGGACCTGCCCGCGCTCCCG
CCGCCTCCAGCGCCCTCAGCCCGGCCGGCCTCTGCAGCGGCACCGCCTCGGCTTCTTCCGCGGCCGCCGG
AGCGGTCGCCATGAACCCCAGCTCCTCGGCGGGAGAGGAGAGAGGGGCGACGGGCGGTAGCAGCAGCAGC
GGAAGCGGCGCCGGGAGCTGCTGCCTGGGCGCCGAGGGCGGCGTGGACCCGCGGGGTGCAGGGGCAGCGG
CGGCTGCTGCCTTGGAGGAGCCCGCGGCCGCCGGACAGAAAGAGAAGGAAGAGGCGCTGGAGGAGAAGCT
GAGGGACTTAACTTTCCGGAAGCAGGTCTCTTACAGGAAAGCCATCTCCCGGACAGGCCTCCAGCATCTG
GCTCCTGCACATCCTCTTGGTCTTCCTGTGGCAAACGGTCCAGCGAAGGAGCCCAGAGCGACTCTGGACT
GGAGTGAGAATGCTGTGAATGGGGAGCACCTATGGCTGGAGACCAACGTCTCGGGGGACCTCTGCTACTT
GGGGGAGGAGAACTGCCAAGTCCGATTTGCAAAATCCGCTCTCAGGAGGAAGTGTGCTGTCTGCAAAATT
GTGGTCCACACCGCCTGCATTGAGCAACTAGAGAAGATTAATTTCAGATGTAAACCTACATTTCGGGAAG
GAGGCTCCAGATCGCCAAGAGAAAACTTTGTGCGCCATCACTGGGTCCACAGGAGGCGGCAGGAGGGAAA
ATGTAAGCAGTGTGGTAAGGGCTTCCAGCAAAAGTTCTCCTTCCACAGTAAAGAGATCGTGGCCATCAGC
TGTTCTTGGTGCAAGCAGGCGTTTCACAATAAAGTGACCTGCTTCATGCTGCATCACATCGAGGAACCCT
GCTCCCTGGGGGCTCATGCTGCTGTTATTGTCCCGCCCACCTGGATCATTAAGGTGAAGAAACCTCAGAA
CTCCCTGAAGGCTTCCAACCGGAAGAAGAAAAGAACAAGTTTTAAAAGGAAAGCCAGTAAAAGAGGAACC
GAGCAGGAAAACAAAGGTCGTCCTTTTGTGATCAAGCCCATCTCCTCTCCTCTCATGAAGCCTTTGCTTG
TCTTTGTGAACCCAAAGAGTGGGGGAAACCAGGGAACCAAAGTCCTACAGATGTTTATGTGGTACCTGAA
TCCACGGCAAGTCTTTGACCTTTCTCAGGAAGGGCCAAAAGATGCGCTGGAGCTGTACAGGAAGGTACCA
AATCTGCGAATTCTGGCCTGCGGTGGGGATGGAACGGTGGGCTGGATCCTCTCCATCCTGGACGAGCTGC
AGCTGAGCCCACAGCCTCCTGTGGGGGTGCTTCCGCTGGGGACTGGGAACGACCTGGCTCGGACGCTCAA
CTGGGGAGGGGGTTACACCGATGAGCCTGTTTCTAAGATCCTGTGCCAGGTGGAAGACGGGACCATTGTA
CAGTTGGATCGCTGGAACCTCCATGTGGAGAGAAACCCAGACTTGCCACCTGAAGAACTTGAAGATGGCG
TGTGTAAGCTTCCTCTGAATGTCTTCAATAATTACTTCAGCCTTGGATTTGACGCTCATGTCACATTGGA
GTTCCATGAATCCCGAGAAGCAAATCCAGAGAAATTCAATAGTCGTTTTCGAAATAAAATGTTCTATGCG
GGGGCAGCCTTTTCTGATTTCCTACAGAGAAGTTCTAGAGATCTATCCAAGCACGTCAAAGTTGTTTGTG
ATGGGACAGACCTCACTCCAAAGATCCAGGACCTGAAGTTCCAGTGTATAGTATTTTTAAATATTCCCAG
ATATTGTGCTGGCACAATGCCTTGGGGAAACCCTGGAGATCATCATGACTTTGAACCCCAGCGTCATGAT
GATGGTTACATTGAAGTCATCGGATTCACTATGGCCTCTTTGGCTGCCCTGCAAGTCGGGGGCCACGGAG
AAAGGTTGCACCAATGTCGGGAAGTGATGCTTCTGACGTACAAGTCCATTCCCATGCAAGTGGACGGGGA
GCCATGTAGGTTGGCGCCTGCTATGATTCGGATCTCCTTGAGGAATCAGGCCAACATGGTACAGAAGAGC
AAGCGGAGAACCTCAATGCCTTTGCTCAATGATCCCCAGTCTGTCCCAGATCGCCTGAGGATCCGCGTGA
ACAAAATCAGTTTACAAGACTATGAAGGACTCCACTATGACAAGGAGAAACTCCGGGAAGCGTCCATCCC
CCTGGGTATTCTAGTTGTTCGTGGAGACTGTGATTTGGAGACTTGCCGTATGTACATAGACCGCCTGCAG
GAGGACTTGCAGTCAGTTTCTTCTGGGTCACAGAGAGTTCATTACCAGGACCAAGAAACTTCCTTCCCCA
GGGCGATCTCAGCTCAGAGGCTGTCCCCACGGTGGTGCTTCCTAGACGCAACTTCTGCTGACCGCTTTTA
TCGAATAGACAGATCTCAGGAACATTTGCACTTTGTGATGGAGATTTCTCATGATGAGATTTTTATTCTG
GACCCAGATATGGTGGTGTCACAGCAGGCAGGGACACCTCCAGGAATGCCAGACCTAGTGGTTGAACAGG
CTTCTGGGCTGTCTGACTGGTGGAACCCTGCCCTGAGGAAACGTATGCTGAGTGACAGTGGGATGATCAC
ACCCCACTATGAGGACTCAGATCTGAAAGACTTCAGCCACTCCAGAGTGCTACAGTCACCAGTCTCTTCG
GAAGATCATGCAATTCTGCAGGCAGTCATAACTGGTGACCTTATGAAGCTCATGGAAAGCTATAAGAATG
GAGGGAGTTTATTAATTCAGGGACCCGGGCACTGTTCCCTCCTTCACTACGCAGCCAAGACTGGCAATGG
GGAGATCGTGAAGTACATCCTTGACCATGGACCTGCGGAATTACTGGACATGGCAGACAGTGAAACGGGT
GAGACTGCACTGCACAAGGCCGCCTGCCAAAGGAACCGGGCTGTATGCCAGCTTCTGGTGGACGCAGGAG
CATCTCTGAGACAGACAGACTCCAAGGGAAAGACACCCCAAGAGCGAGCACAGCAGGCTGGGGACCCGGA
TTTGGCTGCTTACCTAGAAAGCCGACAGAACTATAAGATCATTGGCCACGAAGACCTGGAAACTGCTGTT
TGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_198782
Insert Size 3153 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_198782.2, NP_942077.2
RefSeq Size 3915 bp
RefSeq ORF 3153 bp
Locus ID 688705
UniProt ID F1MAB7
Gene Summary member of the family of diacylglyceride kinases which catalyze the phosphorylation of diacylglyceride to yield phosphatidic acid; involved in inositol lipid-mediated cell signaling [RGD, Feb 2006]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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