Rapgef2 (NM_001107684) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	nRap GEP; PDZ-GEF1; RA-GEF-1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN214025 representing NM_001107684 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAAACAGCTTTGGTGTTTCGCCCACCATGGACAAAGAGTACATGAAAGGAGTCATGAGGACAAAGG TGGATGACTGCCAGTTTGTCTGCATTGCCCAGCAAGATTACTGCCGTATTTTGAACCAAGTAGAAAAGAA CATGCAAAAAGTTGAGGAAGAAGGAGAGATTGTAATGGTGAAGGAGCACCGAGAACTTGATCGGACTGGA ACAAGGAAGGGCCACATCGTCATCAAGGGCACCTCAGAGAGGCTAACCATGCATTTGGTGGAAGAGCATT CTGTAGTGGACCCAACATTCATAGAAGACTTCCTGTTGACATACCGGACTTTCCTTTCCAGCCCAATGGA AGTGGGCAAGAAGTTATTGGAGTGGTTCAATGACCCGAGCCTCAGGGATAAGGTGACACGGGTAGTATTA CTGTGGGTGAATAATCACTTCAATGACTTTGAAGGAGATCCTGCAATGACTCGATTTCTAGAAGAATTTG AAAATAATCTGGAAAGAGAGAAAATGGGTGGACATCTAAGGCTGCTAAACATTGCATGTGCAGCGAAAGC CAAACGAAGGTCGATGACGTTAACAAAGCCGTCCCGGGAGGCGCCTCTGCCCTTCATCCTGCTGGGAGGC TCTGAAAAGGGCTTCGGCATCTTTGTGGACAGTGTTGACTCATCGAGCAAAGCCACTGAAGCAGGCTTGA AGCGTGGGGATCAGATATTAGAAGTGAATGGTCAAAATTTTGAAAATATCCAACTTTCAAAGGCAATGGA AATTCTTAGAAATAACACACACCTGTCCATCACTGTGAAAACCAACTTATTTGTATTTAAAGAACTTCTG ACGAGATTGTCTGAAGAGAAAAGAAATGGTGCCCCCCACCTTCCTAAAATTGGAGACATCAAGAAGGCCA GTCGCTACTCCATACCGGATCTTGCTGTAGATGTAGAACAGGTGATAGGCCTCGAGAAGGTCAACAAGAA AAGCAAAGCCAACACTGTGGGGGGAAGAAACAAGCTAAAGAAAATACTCGACAAGACTCGGATCAGTATC TTACCTCAGAAACCATACAATGATATTGGGATTGGTCAGTCTCAAGATGATAGCATCGTAGGACTGAGGC AGACCAAGCACATTCCAGCTGCCCTGCCTGTCAGTGGAACGCTGTCTTCCAGTAACCCTGACTTGCTGCA GTCACACCACCGGATTCTGGACTTCAGCACTACTCCTGACTTGCCAGATCAGGTACTGAGAGTTTTCAAG GCTGACCAGCAAAGCAGATACATCATGATCAGTAAGGACACCACAGCAAAGGAAGTGGTCATCCAGGCCA TCAGAGAGTTTGCTGTTACTGCCACCCCAGAGCAGTATTCCCTCTGTGAGGTCTCGGTCACGCCAGAGGG AGTTATCAAACAACGAAGGCTCCCAGATCAACTTTCCAAGCTTGCTGACAGAATACAGCTGAGCGGGAGG TATTATCTGAAAAACAACATGGAAACAGAAACGCTTTGCTCAGATGAAGATGCCCAGGAGCTGTTGCGAG AGAGCCAAATTTCACTTCTTCAGCTCAGCACTGTGGAGGTCGCCACACAGCTCTCCATGAGGAACTTTGA ACTCTTCCGCAACATTGAGCCTACTGAATACATAGATGATTTATTTAAACTCAAATCAAAAACCAGCTGT GCCAACCTAAAGAAATTTGAGGAAGTCATTAACCAGGAAACATTTTGGGTGGCTTCTGAAATTCTCCGAG AGACAAACCAGCTGAAGAGAATGAAGATCATTAAGCATTTCATCAAGATTGCCTTGCACTGTAGGGAATG CAAGAATTTTAACTCCATGTTTGCAATCATCAGTGGCCTAAACCTGGCACCAGTGGCAAGACTACGGACC ACCTGGGAAAAACTTCCCAATAAATATGAAAAGCTCTTCCAGGACCTACAAGACCTGTTTGATCCTTCCA GAAACATGGCTAAGTATCGCAATGTCCTGAGTGGCCAGAACTTACAGCCTCCTGTCATTCCCCTATTCCC CGTCATCAAGAAGGACCTCACGTTTCTCCATGAGGGAAATGACTCAAAAGTAGATGGGCTGGTCAACTTT GAGAAGCTGAGGATGATTGCAAAGGAAATCCGTCATGTTGGCCGAATGGCCTCAGTCAACATGGACCCTG CCCTTATGTTCAGAACTCGGTCGCTGAGTCAGGGCAGTGCGAATGCCACAGTGCTGGATGTTGCTCAGAC AGGCGGGCATAAAAAGCGGGTACGCCGTAGTTCCTTCCTCAATGCCAAAAAGCTGTATGAAGATGCCCAA ATGGCTCGGAAAGTGAAGCAATACCTGTCCAACCTGGAGCTGGAGATGGACGAGGAGAGTCTTCAGACAC TGTCACTTCAGTGTGAACCAGCAACCAGCACATTGCCCAAGAACCCTGCAGACAGGAAGCCTGTCAAGTC CGAGACCTCCCCAGTGGCTCCACGTGCAGGGCCCCAGCAGAAAGCGCAGCCTCAGCAGCCGTTGGCACCC CCTCAACCATCGCATAAAGTCAGCCAGGGGCTGCAGGTGCCTGCTGTGTCCCTCTACCCCTCACGAAAGA AGGTGCCAGTGAAGGATCTGCCGCCCTTTGGCATAAACTCTCCACAAGCTTTAAAGAAAATCCTGTCCTT GTCTGAAGAAGGGAGTTTGGAGCGTCATAGGAAGCAAGCAGAAGACACAATATCAAACGCATCCTCACAG CTCTCATCACCCCCCACCTCACCTCAGAGCTCTCCAAGGAAAGGGTATGCATTGGCTCTCAGTGGGACTG TGGATAATTTCTCAGATTCTGGTCACAGTGAGATTTCTTCACGATCCAGTATTGTCAGCAATTCTTCTTT TGACTCGGTGCCAGTCTCTCTGCATGATGAACGGCGCCAGAGGCATTCTGTCAGCATTGTGGAGAGCAAC CTAGGGGTGGGCAGGATGGAGCGGCGGACCCTGATGGAGCCTGATCAATACAGCCTAGGATCATATGCCC CAGTGTCTGAAAGTCGAGGCCTGTATGCCGCAGCGACAGTCATCTCTTCTCCTAGCACAGAGGAGCTTTC CCACGACCAAGGGGACCGTGCTTCTCTCGATGCTGCTGACAGCGGTCGGGGGAGCTGGACATCATGCTCA AGTGGTTCTCATGACAACATACAGACCATCCAGCACCAGAGAAGCTGGGAAACACTGCCATTTGGGCACA CTCACTTTGACTATTCAGGGGATGCGACAGGTATATGGGCCTCCGGCGGCCATATGGACCAAATCATGTT TTCTGATCATAGCACAAAATATAATAGACAGAATCAGAGTAGAGAGAGTCTTGAACAAGCCCAATCCCGG GCAAGCTGGGCGTCTTCCACGGGCTACTGGGGAGAAGACTCAGAAGGCGACACGGGCACTATCAAGCGAA GGGGTGGGAAGGATGTCTCCACTGAGGCAGAGAGCAGCAGCATGGTGCCTGTGACTACAGAGGAAGCTAA GCCTGTCCCTATGCCTGCCCACATAGCCGTGACGCCCAGCACTACCAAGGGACTCATCGCACGAAAGGAA GGCAGGTACCGGGAGCCGCCTCCCACACCTCCAGGCTACGTGGGCATTCCCATTGCCGATTTCCCAGAAG GGCCTTGCCACCCCGCCAGGAAGCCCCCGGATTACAACGTGGCCCTGCAGCGGTCCCGCATGGTGGCACG GCCCACTGAGGCCCCCGCACCAGGCCAGACGCCGCCGGCAGCCACAGCCAGCCGGCCGGGCAGCAAACCG CAGTGGCACAAGCCCAGCGACGCAGACCCACGCCTCGCGCCCTTCCAGCCGCAGGGCTTCGCCGGAGCGG AGGAGGACGAAGATGAACAAGTGTCTGCTGTTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001107684
Insert Size	3885 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001107684.1, NP_001101154.1
RefSeq Size	6311 bp
RefSeq ORF	3885 bp
Locus ID	310533
UniProt ID	F1M386
Gene Summary	Functions as a guanine nucleotide exchange factor (GEF), which activates Rap and Ras family of small GTPases by exchanging bound GDP for free GTP in a cAMP-dependent manner. Serves as a link between cell surface receptors and Rap/Ras GTPases in intracellular signaling cascades. Acts also as an effector for Rap1 by direct association with Rap1-GTP thereby leading to the amplification of Rap1-mediated signaling. Shows weak activity on HRAS. It is controversial whether RAPGEF2 binds cAMP and cGMP or not. Its binding to ligand-activated beta-1 adrenergic receptor ADRB1 leads to the Ras activation through the G(s)-alpha signaling pathway. Involved in the cAMP-induced Ras and Erk1/2 signaling pathway that leads to sustained inhibition of long term melanogenesis by reducing dendrite extension and melanin synthesis. Provides also inhibitory signals for cell proliferation of melanoma cells and promotes their apoptosis in a cAMP-independent nanner. Regulates cAMP-induced neuritogenesis by mediating the Rap1/B-Raf/ERK signaling through a pathway that is independent on both PKA and RAPGEF3/RAPGEF4. Involved in neuron migration and in the formation of the major forebrain fiber connections forming the corpus callosum, the anterior commissure and the hippocampal commissure during brain development. Involved in neuronal growth factor (NGF)-induced sustained activation of Rap1 at late endosomes and in brain-derived neurotrophic factor (BDNF)-induced axon outgrowth of hippocampal neurons. Plays a role in the regulation of embryonic blood vessel formation and in the establishment of basal junction integrity and endothelial barrier function. May be involved in the regulation of the vascular endothelial growth factor receptor KDR and cadherin CDH5 expression at allantois endothelial cell-cell junctions.[UniProtKB/Swiss-Prot Function]

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